Synthesis method of L-alpha-glyceryl phosphoryl choline

Abstract

The embodiment of the invention discloses a synthesis method of L-alpha-glyceryl phosphoryl choline. The synthesis method comprises the following steps of: adding lecithin into a methanol solution, adding an alkali metal alkoxide catalyst into the solution, and performing hydrolysis reaction to remove an acyl group; regulating the pH of the solution to 5-6 by using an organic acid or an inorganic acid; extracting the solution after regulating the pH by using petroleum ether, and removing fat-soluble impurities in the solution; further removing inorganic salts and the impurities through strongly acidic, strongly alkaline, weakly acidic and weakly alkaline resin to obtain a sticky matter-like L-alpha-glyceryl phosphoryl choline crude product; and dissolving the sticky matter-like L-alpha-glyceryl phosphoryl choline crude product with methanol, adding acetone to precipitate crystals, and then performing suction filtration and drying to obtain a white solid finished product. The synthesis method disclosed by the invention has the advantages of simple process, low price of raw materials, easiness in acquisition of the raw material, simplicity in purification, low equipment requirements and easiness in implementation of industrial transformation.

Description

technical field [0001] The invention relates to the field of glycerol phosphorylcholine, in particular to a method for synthesizing L-alpha-glycerophosphorylcholine. Background technique [0002] The structure of L-α-glycerophosphorylcholine is simple, and the literature reports are mainly obtained by total synthesis or hydrolysis of soybean lecithin. There are many literature reports at home and abroad about the preparation method of GPC, such as the literature J.Am.Chem.Soc ., 1948, 70, 1394-1399; Kanu M. Petl, et al. , Journal of Lipid Research, 1979, Vol20, 674-677; Patent ES2019552A6; Patent US5250719, US5433833A. In the preparation process disclosed in the above-mentioned documents, the target compound is synthesized through the formation of phospholipid bonds after using the total synthesis method, generally using glycerol or other compound skeletons as precursors, after long-chain alkylation or acylation. Its characteristic is that there are many synthetic steps an...

AI Technical Summary

Problems solved by technology

Its characteristic is that there are many synthetic steps and little flexibility. Generally, protective groups are used. In addition to the preparation of general basic phospholipids, optical activity experiments are also carried out; there are also reports in the literature that soybean lecithin is hydrolyzed and then converted into chromium chloride salt to prepare GPC. , the reaction is relatively simple for total synthesis, but it is not easy to purify, and the post-treatment involves the removal of highly toxic chromium chloride, which is not suitable for industrialization