Protein chip and preparation and detection method thereof

Abstract

The invention provides a protein chip. The affinity of histidine labels and cations is utilized to fix protein to be fixed on a chip solid phase carrier on a chip carrier. The combined mode basically does not generate influences to functions of protein; meanwhile the carrying of the histidine labels simplifies the separation purification of protein as well as a fixing method of an SPR chip; the preparation of the protein chip is simple and is easy to implement; and the protein chip is low in cost, and high in throughput.

Description

Technical field [0001] The present invention belongs to the field of protein chips, and in particular relates to a protein chip that fixes the protein to be immobilized on the chip solid carrier on the solid carrier through the affinity between the histidine tag and the cation on the solid carrier and Its preparation and detection methods. Background technique [0002] In the life activities of the human body or other organisms, proteins, as the main performers of biological functions, participate in and dominate many biological activities in the body, including protein-protein interactions, enzymes catalyze the reactions of substrates and generate new products, antibody proteins and The interaction between a variety of antigens from different sources, the interaction between proteins and nucleic acids and other biological macromolecules. More importantly, the interaction between proteins and different small molecules is mainly reflected in the interaction of hormones and recept...

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Problems solved by technology

However, these methods generally have disadvantages such as high cost, complicated operation, and low efficiency.

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