Protein chip and preparation and detection method thereof

A technology of protein chips and protein chips, which is applied in the field of protein chips, can solve the problems of complex operation, high cost, and low efficiency, and achieve the effects of improving sensitivity, reducing the demand for quantity, and reducing costs

Inactive Publication Date: 2013-07-10
SHENZHEN QIANHAI ICECOLD IT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods generally have disadvantages such

Method used

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  • Protein chip and preparation and detection method thereof
  • Protein chip and preparation and detection method thereof

Examples

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Embodiment

[0038] Example: Preparation of a protein chip using platelet-derived growth factor (PDGF) as a protein immobilized on a solid-phase carrier of the chip and an experimental method for using the chip to screen small molecule compounds with high efficiency binding thereto.

[0039] 1. Reagents and instruments:

[0040] Human liver cancer cell line (SMC-7721), E.coli M15 cell line, and pQE-30 vector are preserved by our company, Taq plus DNA polymerase was purchased from Tiangen Biochemical, restriction enzymes were purchased from Promega, HBS-EP buffer Liquid (0.01mol / L HEPES, pH=7.4, 0.15mol / L NaCl, 3mmol / L EDTA, Surfactant P20 with a mass fraction of 0.005%), Glycine-HCl, [N-ethyl-N′-(3-二 Ethylaminopropionic acid)] carbodiimide (EDC), succinimide (NHS) and ethanolamine hydrochloride were purchased from Amersham Biosciences, trypsin, matrix α-cyano-4-hydroxycinnamic acid (CHCA) ), trifluoroacetic acid (TFA), and 3,5-dimethoxy-4-hydroxycinnamic acid (SA) were purchased from Sigma. Ace...

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Abstract

The invention provides a protein chip. The affinity of histidine labels and cations is utilized to fix protein to be fixed on a chip solid phase carrier on a chip carrier. The combined mode basically does not generate influences to functions of protein; meanwhile the carrying of the histidine labels simplifies the separation purification of protein as well as a fixing method of an SPR chip; the preparation of the protein chip is simple and is easy to implement; and the protein chip is low in cost, and high in throughput.

Description

Technical field [0001] The present invention belongs to the field of protein chips, and in particular relates to a protein chip that fixes the protein to be immobilized on the chip solid carrier on the solid carrier through the affinity between the histidine tag and the cation on the solid carrier and Its preparation and detection methods. Background technique [0002] In the life activities of the human body or other organisms, proteins, as the main performers of biological functions, participate in and dominate many biological activities in the body, including protein-protein interactions, enzymes catalyze the reactions of substrates and generate new products, antibody proteins and The interaction between a variety of antigens from different sources, the interaction between proteins and nucleic acids and other biological macromolecules. More importantly, the interaction between proteins and different small molecules is mainly reflected in the interaction of hormones and recept...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N1/28G01N27/62G01N21/55G01N21/552G01N27/626
Inventor 卢志强池静
Owner SHENZHEN QIANHAI ICECOLD IT CO LTD
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