Method for producing dzo gender controllable in vitro embryos

A technology of gender and embryos, applied in the field of in vitro embryo production with controllable gender, can solve problems that have not yet been seen, and achieve the effect of increasing the number

Inactive Publication Date: 2013-07-24
SICHUAN ACAD OF GRASSLAND SCI
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no known method of using in vitro fertilization for interspecific hybridization to produce in vitro embryos with controllable sex

Method used

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Embodiment Construction

[0020] Below in conjunction with specific embodiment the present invention is described in further detail:

[0021] Yak ovaries were collected from the slaughterhouse, placed in sterile saline at 30°C, and brought back to the laboratory as soon as possible with a thermos bottle. Wash the ovaries with sterile normal saline for 3 to 4 times, and extract oocytes from follicles with a diameter of 2 to 8 mm on the surface of the ovary with a No. 8 syringe. Under a stereo microscope, select oocytes with more than three layers of dense granulosa cells, bright appearance, and uniform cytoplasm, and then use Hepes buffered oocytes with TCM199 supplemented with 5mmol / L NaHCO3 and 3% OCS (estrous bovine serum) The collected fluid was washed 2 to 3 times, and then washed once with Hepes-buffered TCM-199+5%OCS+0.1μg / mL FSH (follicle-stimulating hormone)+3%BFF (bovine follicle fluid) maturation culture medium. Transfer the washed oocytes into a petri dish added with serum, hormone, follicu...

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Abstract

The invention discloses a method for producing dzo gender controllable in vitro embryos. The method comprises the steps of: removing connective tissues surrounding a Bos grunniens ovary and conducting cleaning, selecting oocytes and washing them; transferring the washed oocytes into a culture dish added with serum, hormone, a follicular fluid and a Hepes buffer solution, and then placing the culture dish into an incubator to perform maturation culture; taking X or Y chromosome-containing sperms from Bos taurus, separating dead sperms from live sperms in semen by a Percoll gradient separation liquid; picking the oocytes that are cultured to maturity from the Hepes buffer solution, washing them with a fertilization fluid, then adding the X or Y chromosome-containing sperms into oocyte-containing fertilization microdroplets, and placing the microdroplets into the incubator to conduct in vitro fertilization culture; after fertilization, carrying out a blastocyst maturation treatment; and at a mature time, recovering the blastocyst, and performing embryo transplantation or storage. By combining the method provided in the invention and other modern embryo production technologies, gender controllable dzo breeding can be carried out, and the number of high quality dzos can be increased rapidly.

Description

technical field [0001] The invention relates to a method for producing in vitro embryos of Y cattle, in particular to a method for producing in vitro embryos of Y cattle with controllable sex. Background technique [0002] Pyak is the offspring of interspecific hybridization between common cattle Bos taurus (♂) and yak Bos grunniens (♀) as the male parent. It has obvious hybrid advantages, and its milk, meat production capacity and service ability are superior to those of yaks. , In addition, it has a long service life and a high market price. It is an important supplement to yak production and is very popular among people. In addition, Pyak plays an extremely important role in people's production and life: First, the castrated male Pyak not only has the same adaptability to the plateau climate and environment as yaks, but also has the advantages of being more docile and easy to control. Therefore, in pastoral areas, P cattle are often used as pack cattle, and in semi-agric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 安添午罗晓林杨平贵官久强吴伟生赵洪文毛德才谢荣清李华德周明亮陈明华蒋世海
Owner SICHUAN ACAD OF GRASSLAND SCI
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