The invention discloses a collecting device for follicular fluid of an animal in vitro. The collecting device comprises a collecting device body, a flexible pipe, a metal pipe, a buffer solution pipe,a thin plug, an air suction pipe, a rubber plug and a collecting bottle, the collecting device body is composed of a handle, a concave cavity, a rubber ring, an upper cover and several puncturing needles, the upper cover is connected with the flexible pipe, and the flexible pipe is connected with the metal pipe; the buffer solution pipe is composed of an injector adapter located at the upper endof the buffer solution pipe, a middle pipe body and a flushing ball located at the lower end of the buffer solution pipe, and several small holes are formed in the flushing ball; the bottle opening ofthe collecting bottle is plugged by the rubber plug, and the ends of the metal pipe, buffer solution pipe and air suction pipe are all inserted in the rubber plug and appear above the collecting bottle; the other end of the air suction pipe is connected with a vacuum pump, the lower end of the collecting bottle is provided with a glass valve, and a liquid outlet hole is formed in the glass valve.The collecting device for the follicular fluid of the animal in vitro can simply conveniently collect the follicular fluid of the animal in vitro, contamination on a sample cannot be caused during the operative process, and a pipeline can be cleaned conveniently.