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Method for analyzing relation between follicular fluid metabolite and oocyte quality

A technology of metabolites and follicular fluid, applied in the field of reproductive medicine, can solve the problems of large subjective influence of observers, inability to accurately reflect the functional state of oocytes, and lack of uniform standards.

Inactive Publication Date: 2019-05-21
BEIJING OBSTETRICS & GYNECOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Morphological methods are non-invasive and convenient, but they lack the most suitable unified standard, cannot accurately reflect the functional state of oocytes, and are greatly influenced by the subjectivity of observers. The subjectivity between observers makes the relationship between oocytes and embryonic development show difference

Method used

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  • Method for analyzing relation between follicular fluid metabolite and oocyte quality
  • Method for analyzing relation between follicular fluid metabolite and oocyte quality
  • Method for analyzing relation between follicular fluid metabolite and oocyte quality

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Experimental program
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Embodiment approach

[0021] According to one embodiment of the present invention, HPLC-MS (high performance liquid chromatography-mass spectrometry) technology is used to measure the content of two groups of differential metabolites, and the differential metabolites that independently affect the quality of oocytes are determined by stepwise logistic regression analysis. , and each metabolite was given a graded score reflecting the ovarian reserve function, based on which an oocyte quality evaluation system was established. The area under the ROC curve was used to evaluate the diagnostic accuracy, and the Kappa test was used to evaluate the diagnostic reliability.

[0022] The innovation of the present invention is:

[0023] 1) Theoretical innovation

[0024] At present, most studies on the screening of differential metabolites in follicular fluid focus on the impact of metabolites on oocyte quality, and no evaluation system for ovarian reserve function has been established. In this study, HPLC-M...

Embodiment 1

[0028] 1. Selection of patients with low ovarian function in the experimental group:

[0029] 1) Diagnostic criteria:

[0030] Diagnose according to the diagnostic criteria for ovarian hypofunction reported in the literature: 12mIU / ml 2-3.6, or bE2 > 80 pg / ml.

[0031] 2) Inclusion criteria:

[0032] ①Meet the diagnostic criteria for ovarian insufficiency.

[0033] ② Infertile patients who need IVF-ET.

[0034] ③Married women aged 25-30.

[0035] ④ Accept IVF-ET ≤ 2 times.

[0036] ⑤ The subjects were informed and voluntarily signed the informed consent.

[0037] 3) Exclusion criteria:

[0038] ①Infertility caused by uterine deformity

[0039] ② Infertile patients with comorbidities such as polycystic ovary syndrome and thyroid dysfunction

[0040] ③ Those with serious diseases of other organs.

[0041] ④ Infertility caused by male factors.

[0042] ⑤ Receive IVF-ET ≥ 3 times.

[0043] 4) Elimination criteria

[0044] ① Exclusion: Subjects who should not be enrolled...

Embodiment 2

[0052] Analysis of metabolic components of follicular fluid based on NMR technology:

[0053] as per figure 1 In the technical route shown, 2ml of frozen follicular fluid is placed in a freeze dryer, and after sublimation and drying, it is made into a freeze-dried powder and sealed for storage. 1 g of the lyophilized powder was precipitated with 75% chromatographic methanol, centrifuged at 12000 r / min for 10 min, the supernatant was taken, dried with nitrogen, dissolved in 350 ul of heavy water, and the hydrogen spectrum was measured by Bruker 800 MHz NMR, and the integral value was calculated. Supervised analysis method partial least squares-discriminant analysis (PLS-DA) was used, and SIMCA-P and MetaProc software were used for OPLS-DA processing. The differential metabolite groups were screened out, searched and searched by the HMDB human metabolomics database, and the differential metabolites were qualitatively analyzed.

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Abstract

The invention relates to a method for analyzing the relation between a follicular fluid metabolite and oocyte quality. The method is characterized by comprising a step of comparing the composition ofthe follicular fluid metabolite of a subject with low ovarian reserve with that of the follicular fluid metabolite of a healthy subject. According to the method in the invention, a characteristic biomarker is screened through metabonomics analysis, the relationship between the follicular fluid metabolism marker group and the oocyte quality is determined, and a more objective and non-invasive oocyte quality evaluation method is established.

Description

technical field [0001] The invention relates to the field of reproductive medicine, in particular to a method for analyzing the relationship between follicular fluid metabolites and oocyte quality. Background technique [0002] Infertility patients in my country have accounted for 12.5% ​​of the population of childbearing age, and the number has exceeded 40 million, showing a trend of rapid growth. Assisted reproductive technology (assisted reproductive technology, ART) has brought hope for infertility patients to give birth to new life bodies. However, in in vitro fertilization (in vitro fertilization, IVF), nearly 80% of embryos fail to implant. In order to improve the success Multiple embryo transfers are carried out in various reproductive centers, resulting in an increase in multiple pregnancies, and complications such as premature birth, stillbirth, low birth weight, birth defects, and mother's high blood pressure in multiple pregnancies are higher than those of natura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08G01N30/02
Inventor 阴赪宏王程荣代荫梅岳文涛杨晓葵洪黎明
Owner BEIJING OBSTETRICS & GYNECOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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