Preparation of cysteine and cu2+ fluorescent probes based on au/ag core/shell quantum dots
A technology of cysteine and fluorescent probes, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as cysteine fluorescent probes that have not yet been seen, and achieve simple methods and low cost Effect
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Embodiment 1
[0023] Cysteine and Cu based on Au / Ag core / shell quantum dots 2+ For the preparation process of fluorescent probe, see figure 1 The detailed preparation steps are as follows: Dissolve chloroauric acid and BSA in 20mL deionized water, adjust the molar concentration of chloroauric acid to 2mM, and the dosage of BSA to 10mg / mL, at 20℃ and magnetic stirring, at pH 7.5 After reacting in phosphate buffer for 6 hours, BSA stabilized Au nanoparticles were prepared. Add this Au nanoparticles to a 20mL homogeneous aqueous solution containing silver nitrate and BSA, adjust the molar concentration of silver nitrate to 1mM, the amount of BSA to 5mg / mL, and the amount of Au nanoparticles to 0.2mg / mL at 20℃. Under magnetic stirring, react for 12h in pH 7.5 phosphate buffer to prepare BSA stable Au / Ag core / shell quantum dots. Through dialysis, rotary evaporation, centrifugal separation, and vacuum drying, the dry quantum dot powder is then dissolved in deionized water and configured into a 0...
Embodiment 2
[0027] The detailed preparation steps are as follows: Dissolve chloroauric acid and BSA in 20mL of deionized water, adjust the molar concentration of chloroauric acid to 5mM, and the dosage of BSA to 20mg / mL, at 25℃ and magnetic stirring, in phosphoric acid at pH 8.0 After reacting in the buffer for 12 hours, BSA stable Au nanoparticles were prepared. Add the Au nanoparticles to a 20mL homogeneous aqueous solution containing silver nitrate and BSA, adjust the molar concentration of silver nitrate to 2mM, the dosage of BSA to 10mg / mL, and the dosage of Au nanoparticles to 0.5mg / mL at 25℃. Under magnetic stirring, it was reacted in a phosphate buffer of pH 8.0 for 24 hours to prepare BSA-stable Au / Ag core / shell quantum dots. Through dialysis, rotary evaporation, centrifugal separation, and vacuum drying, the dry quantum dot powder is then dissolved in deionized water and configured into a 0.5 mg / mL quantum dot solution for later use. Add different molar concentrations of cystein...
Embodiment 3
[0029] The detailed preparation steps are as follows: Dissolve chloroauric acid and BSA in 20mL of deionized water, adjust the molar concentration of chloroauric acid to 8mM, and the dosage of BSA to 30mg / mL, at 37°C and magnetic stirring, in phosphoric acid at pH 8.5 After 18 hours of reaction in the buffer, BSA stable Au nanoparticles were prepared. Add the Au nanoparticles to a 20mL homogeneous aqueous solution containing silver nitrate and BSA, adjust the molar concentration of silver nitrate to 3mM, the dosage of BSA to 15mg / mL, and the dosage of Au nanoparticles to 0.8mg / mL at 37℃. Under magnetic stirring, the reaction was carried out in a phosphate buffer of pH 8.5 for 36 hours to prepare BSA-stable Au / Ag core / shell quantum dots. Through dialysis, rotary evaporation, centrifugal separation, and vacuum drying, the dry quantum dot powder is then dissolved in deionized water and configured into a 0.5 mg / mL quantum dot solution for later use. Add different molar concentrati...
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