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Novel high-efficiency extraction process for astaxanthin in Haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the direction of organic chemistry and the like, can solve the problems that ultra-high pressure is difficult to achieve industrial application conditions, ultrasonic energy consumption is large, product concentration is low, etc., and the extraction method is simple and easy to produce. Low cost and small dosage

Active Publication Date: 2015-04-29
INNOBIO CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process is complicated, the steps are cumbersome, there are many types of solvents used, and mixed solvents are produced, which is difficult to recover. It can be seen from the process that the efficiency of wall breaking is low
Wan Qingjia, Guo Wenjing, etc. [1~2] used 200~300MPa to break the wall, and the breaking efficiency is high, but the ultra-high pressure used is difficult to meet the industrial application conditions
Jiang Ling et al [3] used acid heat plus ultrasonic wave to break the wall, and then used sesame oil for ultrasonic extraction, the extraction rate could reach more than 97%, but the energy consumption of ultrasonic wave was large, and astaxanthin extract was mixed with high boiling point oil, Difficult to separate, low product concentration

Method used

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  • Novel high-efficiency extraction process for astaxanthin in Haematococcus pluvialis
  • Novel high-efficiency extraction process for astaxanthin in Haematococcus pluvialis
  • Novel high-efficiency extraction process for astaxanthin in Haematococcus pluvialis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]①Take 20g of dried Haematococcus pluvialis, in which the astaxanthin content is 1.64%, add the dried Haematococcus pluvialls to 100ml of 8% sulfuric acid aqueous solution, put it in a water bath at 80°C and stir for 30min, slowly add 10% NaOH dropwise Stir the aqueous solution continuously, adjust the pH value to neutral, filter, add the filter cake to 100ml 95% ethanol, stir at room temperature for 10min, filter to remove the filtrate, and obtain broken-walled Haematococcus pluvialis;

[0040] ② Stir the broken Haematococcus pluvialis with 100ml and 60ml of ethyl acetate at 50°C for 10 minutes, filter, combine the filtrate, and concentrate in vacuum at 50°C to obtain 5.2g of astaxanthin extract, of which the astaxanthin content is 6.1% , The extraction rate of astaxanthin is 96.7%.

Embodiment 2

[0042] ①Take 20g of dried Haematococcus pluvialis, in which the content of astaxanthin is 1.73%, add the dried Haematococcus pluvialls to 100ml of 10% hydrochloric acid aqueous solution, put it in a 70°C water bath and stir for 20min, slowly add 10% NaOH dropwise Stir the aqueous solution continuously, adjust the pH value to neutral, filter, add the filter cake to 100ml 93% ethanol, stir at room temperature for 10min, filter to remove the filtrate, and obtain broken-walled Haematococcus pluvialis;

[0043] ②The broken Haematococcus pluvialis was extracted with 100ml and 60ml of ethyl acetate:ethanol:acetone 5:3:1 (V:V:V) mixed organic reagent at 50°C for 10min, filtered, and the filtrate was combined. Vacuum concentration at 50°C yielded 5.8 g of astaxanthin extract, wherein the astaxanthin content was 5.7%, and the astaxanthin extraction rate was 95.5%.

Embodiment 3

[0045] ①Take 20kg of dried Haematococcus pluvialis, in which the astaxanthin content is 1.91%, add the dried Haematococcus pluvialls to 110kg of 10% glacial acetic acid aqueous solution, stir at 60°C for 30min, slowly add 10% NaOH aqueous solution dropwise and Stir continuously, control the temperature below 50°C, adjust the pH value to neutral, filter, add the filter cake to 100kg of 92% ethanol, stir at 40°C for 30 minutes, filter and remove the filtrate, and obtain broken-walled Haematococcus pluvialis;

[0046] ② Broken Haematococcus pluvialis was sequentially extracted with 90kg, 50kg, and 50kg of ethyl acetate at 40°C for 30 minutes, filtered, collected the filtrate, and concentrated in vacuum at 60°C to obtain 5.6kg of astaxanthin extract, of which the astaxanthin content was 6.2 %, the extraction rate of astaxanthin is 90.9%.

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Abstract

The invention discloses a process for extraction of astaxanthin in Haematococcus pluvialis. According to the process, wall-broken Haematococcus pluvialis is obtained by using an acid method, and then an astaxanthin extract is obtained through ethanol dehydration and organic solvent extraction. The wall breaking and extraction process provided by the invention has the advantages of easiness, high efficiency, convenient operation, low energy consumption and controllable quality, and the wall breaking rate and the extraction ratio of Haematococcus pluvialis can reach more than 95%. The astaxanthin extract prepared by using the process is a dark red oily viscous substance and can meet requirements for raw materials needed in production of health food, cosmetics and medicines.

Description

technical field [0001] The invention relates to an extraction process of astaxanthin, in particular to a process for extracting astaxanthin from Haematococcus pluvialis. Background technique [0002] Astaxanthin is a non-provitamin A carotenoid with an o-hydroxyketone structure. It has a super antioxidant function. Its antioxidant capacity is 500 times that of vitamin E and 20 times that of β-carotene. , It has a good curative effect on skin cancer caused by ultraviolet rays. Clinical and animal experiments have shown that astaxanthin has important biological functions such as anti-inflammation, anti-tumor, anti-aging, enhancing immunity, and preventing cardiovascular diseases. In addition, astaxanthin also has a significant coloring function, and is mainly used as a coloring agent for aquatic products, poultry eggs, and ornamental animals. Therefore, astaxanthin has broad market prospects in industries such as food, medicine, cosmetics, health care products and beverages....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
Inventor 吴文忠王建华徐维锋张显仁邵天文
Owner INNOBIO CORP LTD
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