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Micro-cantilever beam array biochemical sensing apparatus and biochemical detection method thereof

A technology of micro-cantilever beam and sensing device, which is applied in the field of biochemical sensing, can solve the problems affecting the consistency of the laser beam emitted by the optical fiber array and the effective detection distance, affecting the quality of spot displacement detection, and low detection sensitivity, so as to improve biochemical detection. The effect of precision, light weight and high scanning efficiency

Inactive Publication Date: 2013-08-07
CHINA ELECTRONIC TECH GRP CORP NO 38 RES INST +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since the fiber array is essentially connected with multiple semiconductor lasers at the back end, its wiring is complicated, there are many accessories, the volume becomes larger, and the flexibility is low; at the same time, the flatness of the fiber end face has a great influence on the divergence angle and spot shape of the outgoing laser beam. The impact is also great, which will greatly affect the consistency of the laser beam emitted by the fiber array and the effective detection distance; in the CCD surface light source detection method, the image will be diffused due to the bending of the tip of the micro-cantilever beam, which seriously affects the detection quality of the spot displacement , resulting in low detection sensitivity

Method used

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  • Micro-cantilever beam array biochemical sensing apparatus and biochemical detection method thereof
  • Micro-cantilever beam array biochemical sensing apparatus and biochemical detection method thereof
  • Micro-cantilever beam array biochemical sensing apparatus and biochemical detection method thereof

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Embodiment 1

[0082] Example 1. Measurement of temperature changes by a micro-cantilever array sensing method based on a vertical cavity surface emitting laser array

[0083] 1. A commercial microcantilever array (German micromotive company, such as image 3 As shown, the microcantilever beam is 500 μm long, 90 μm wide, and 1 μm thick, the surface is coated with a 0.02 μm thick gold layer, and the center-to-center distance between two microcantilever beams is 250 μm) fixed to the system (including VCSELs, Biochemical reaction pool, temperature controller, PSD, A / D converter, computer, such as figure 1 Shown) in the biochemical reaction pool.

[0084] 2. The laser beam emitted by the vertical cavity surface emitting laser array periodically scans two fixed points with a distance of 250 μm on the substrate of the microcantilever array for 9 hours, and the scanning displacement curve is shown in Figure 4a and Figure 4b As shown: In the X direction and Y direction, the two scanning positio...

Embodiment 2

[0086] Example 2. Detection of Clenbuterol by Microcantilever Array Biochemical Sensing Method Based on Vertical Cavity Surface Emitting Laser Array

[0087] 1. Experimental Setup and Reagents

[0088] The specific structure of the detection system is as follows: figure 1 As shown in , it mainly includes vertical cavity surface emitting laser array (parameters: 2 arrays, height 160μm, width 250μm, total length 500μm, distance between adjacent laser centers 250μm, maximum input voltage 5V, wavelength of emitted laser is 650nm), biochemical reaction pool (volume 0.5mL, fully sealed, mainly composed of a sample inlet 11 with an inner diameter of 1mm, a sample outlet 12, a glass slide 13, and a micro-cantilever beam array fixed platform 14, Figure 7 ), temperature controller (temperature control accuracy 0.01℃, temperature control range 0℃~100℃), PSD (displacement resolution 1μm, target surface size 22mm×22mm), A / D converter (16 bits), computer.

[0089] Clenbuterol antibody, c...

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Abstract

The present invention relates to a micro-cantilever beam array biochemical sensing apparatus and a biochemical detection method. The micro-cantilever beam array biochemical sensing apparatus comprises a biochemical reaction cell, a micro-cantilever beam array, a vertical cavity surface emitting laser (VCSEL) array, a signal generator, a photoelectric position sensitive detector (PSD) and a data treatment device. According to the apparatus, a laser beam emitted by the VCSEL array is adopted to periodically scan the micro-cantilever beam array, an optical lever principle is adopted to amplify bending deformation signals of various micro-cantilever beams in the micro-cantilever beam array, and the PSD is adopted to perform time program detection receiving so as to real-timely monitor biochemical reaction process information on various micro-cantilever beams. With the present invention, highly sensitive, rapid and parallel detection on the biochemical reaction information of the micro-cantilever beam array can be achieved, and the apparatus can be used for monitoring and detection in food safety, environmental pollution, bio-medicine, scientific researches, production manufacturing and other fields. The invention further discloses a biochemical detection method of the micro-cantilever beam array biochemical sensing apparatus.

Description

technical field [0001] The invention belongs to the technical field of biochemical sensing, and in particular relates to a micro-cantilever beam array biochemical sensing device and a biochemical detection method of the micro-cantilever beam array biochemical sensing device. The device and method can be applied to food safety and environmental pollution Monitoring and detection in the fields of biomedicine, scientific research and manufacturing. Background technique [0002] Microcantilever biochemical sensing technology based on surface stress detection is a new sensing technology that has emerged in recent years. On one side of the gold-plated layer, when the target molecules in the detected sample liquid react specifically with the probe molecules on the gold surface of the micro-cantilever, the surface stress of the micro-cantilever will change, resulting in bending deformation of the micro-cantilever. By detecting this deformation process by optical or electrical metho...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 邬林胡国俊张加波时凯王红军
Owner CHINA ELECTRONIC TECH GRP CORP NO 38 RES INST
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