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Method for extracting silybum marianum protein

An extraction method and technology of milk thistle, applied in the application field of biotechnology in food, can solve the problems of emulsification of processing performance, influence of foaming, insufficient protein extraction, etc., achieve significant economic and social benefits, improve The effect of extraction rate

Inactive Publication Date: 2013-08-14
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current protein extraction method is mainly alkali-soluble acid precipitation method, but due to the influence of plant cell walls, protein extraction is not sufficient
In recent years, protease preparations have also been used in the extraction of plant proteins. It improves the protein extraction rate by hydrolyzing the protein into smaller molecular polypeptides, but the processing properties of the products obtained by this method (such as emulsification, foaming) will be affected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] After pulverizing the milk thistle seeds, degrease according to the method (a method for comprehensive utilization of milk thistle seeds, China, ZL201010152017.0), and pass the obtained defatted powder through a 60-mesh sieve;

[0016] Milk thistle seed defatted powder and distilled water were mixed at a mass-to-volume ratio (w / v) of 1:16 (g / mL), and then cellulase and amylase were added at a concentration of 7.5% by mass ratio of enzyme to substrate. Mixed enzymes, wherein the mass ratio of cellulase and amylase is 1:2, under the conditions of solution pH 5.5, enzyme pretreatment temperature 50 ℃, after 60 min of enzymolysis, heat to inactivate the enzyme, adjust the pH value to 10, alkali The temperature of the extraction water bath is 50 ℃, leaching for 60 minutes, centrifugation, the supernatant is adjusted to pH 5.5 with hydrochloric acid, centrifuged again and the precipitate is collected, the pH of the precipitation is adjusted to neutral, vacuum freeze-drying is ...

Embodiment 2

[0018] After pulverizing the milk thistle seeds, degrease according to the method of (A method for comprehensive utilization of milk thistle seeds, China, ZL201010152017.0), and pass the obtained defatted powder through a 60-mesh sieve;

[0019] Milk thistle seed kernel defatted powder and distilled water were mixed according to the mass to volume ratio (w / v) of 1:16 (g / mL), and then the concentration of cellulase and amylase was added according to the concentration of enzyme and substrate mass ratio of 9%. Mixed enzymes, wherein the mass ratio of cellulase and amylase is 1:2, under the conditions of solution pH 6.5, enzyme pretreatment temperature 50 ℃, after 60 min of enzymolysis, heat to inactivate the enzyme, adjust the pH value to 10, alkali The temperature of the extraction water bath is 50 ℃, leaching for 60 minutes, centrifugation, the supernatant is adjusted to pH 5.5 with hydrochloric acid, centrifuged again and the precipitate is collected, the pH of the precipitatio...

Embodiment 3

[0021] After pulverizing the milk thistle seeds, degrease according to the method in (A method for comprehensive utilization of milk thistle seeds, China, ZL201010152017.0), and pass the obtained defatted powder through a 60-mesh sieve;

[0022] Milk thistle seed kernel defatted powder and distilled water were mixed according to the mass to volume ratio (w / v) of 1:16 (g / mL), and then the concentration of cellulase and amylase was added according to the concentration of enzyme and substrate mass ratio of 9%. Mixed enzymes, wherein the mass ratio of cellulase and amylase is 1:2, under the conditions of solution pH 6.0, enzyme pretreatment temperature 50 ℃, after 150 min of enzymolysis, heat to inactivate the enzyme, adjust the pH value to 10, alkali The temperature of the extraction water bath is 50 ℃, leaching for 60 minutes, centrifugation, the supernatant is adjusted to pH 5.5 with hydrochloric acid, centrifuged again and the precipitate is collected, the pH of the precipitati...

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PUM

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Abstract

The invention discloses a method for extracting silybum marianum protein and belongs to the technical field of application of biotechnology in foods. The method comprises the following steps: dissolving silybum marianum seed kenel spent meal in distilled water, adding mixed enzyme of cellulose and amylase according to a mass ratio of the enzyme to the substrate of 4.5-12 percent so as to carry out enzymolysis for 0.5-3 hours under the conditions of the pH of 4-7 and the temperature of 40-70 DEG C, heating and inactivating the enzymes, regulating the pH value of the solution to be 10 at the water bath temperature of 50 DEG C, leaching for 60 minutes, centrifuging, regulating the pH value of the liquid supernatant to be 5.5 by using hydrochloric acid, centrifuging and collecting the precipitate, dissolving the precipitate by using water, regulating the pH to be neutral, and performing vacuum freeze drying to obtain the silybum marianum protein powder. The silybum marianum seed kenel spent meal is treated by employing cellulose and amylase, and the cellulose and amylase can hydrolyze cellulose, starch and other polysaccharide components, so that the constraint of the polysaccharide on the protein can be relieved, the dissolution of the protein is promoted, and the protein extracting rate is improved.

Description

technical field [0001] The invention relates to a method for extracting milk thistle protein, in particular to a method for extracting milk thistle protein through pretreatment with cellulase and amylase, and then alkali-dissolving and acid-precipitating method, which belongs to the technical field of application of biotechnology in food. Background technique [0002] Milk thistle is a medicinal herb of Compositae. As a traditional medicinal plant, milk thistle mainly uses its seeds. Milk thistle seeds are mainly used to extract and separate its main component, silymarin, and then purify into silybin. Silybin is mainly found in the seed shell, while silymarin oil and protein are found in the kernel. Studies have proved that the protein content in milk thistle seed kernel defatted powder is as high as about 47%, and the amino acids in milk thistle protein are complete, which is a high-quality protein, and milk thistle protein can be prepared by enzymatic hydrolysis. peptide...

Claims

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Application Information

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IPC IPC(8): C07K1/30
Inventor 朱淑云董英秦云云张珊珊
Owner JIANGSU UNIV
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