Detection method of nafil medicines and detection kit

Abstract

The invention discloses a rapid detection method of nafil medicines, which successively comprises the following steps of: (1) taking a sample, and adding an acid solution to dissolve the sample; (2) adding an extraction agent to extract, and adding a buffer solution into extract; and (3) dropping a colour developing agent, and observing whether the solution is yellow or not, wherein if the solution is yellow, the sample contains nafil medicines, or else the sample does not contain nafil medicines. The invention further provides a special detection kit based on the method. The rapid detection method disclosed by the invention has the advantages of being low in analysis cost, free from special equipment, strong in specificity and high in sensitivity and is applied to testing whether nafil medicines are doped in medicines and health-care products in field; and the disadvantages in the prior art are overcome.

Description

technical field [0001] The invention relates to a detection method and a detection kit for nafil drugs. Background technique [0002] Phosphodiesterase is a negative regulator of the NO-cGMP pathway. It reduces the effect of nitric oxide by catalyzing the decomposition of cGMP. It is generally believed that nitric oxide in the body is a factor that regulates the expansion of vascular smooth muscle, so phosphodiesterase The result of the action is to promote the contraction of vascular smooth muscle. Nafil drugs can highly selectively inhibit the activity of phosphodiesterase type 5 (PDE5) in the human body. PDE5 is expressed at a very high level in the cavernous body of the penis, while its expression is low in other tissues and organs of the human body. After taking nafil, the smooth muscle of the corpus cavernosum in the penis relaxes under the action of the drug, the blood flow increases, the corpus cavernosum is congested, and the penis becomes erect, thereby producing ...

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: (1) The chromatographic resolution is low, the ingredients of health care products are complex, and there are many interference factors. It is easy to misjudge some coexisting ingredients as nafil drugs. Proximity with nafil drugs will interfere with the detection of target components

(2) The time for chromatographic development and drying is long, which cannot meet the requirements of rapid determination

(4) A fixed experimental site is required, and it is not suitable for on-site testing with high mobility

Its disadvantages are: (1) The instrument is expensive, and the sample pretreatment time is long. Most of them are for a single product, and the mobile phase used is mostly a certain proportion of methanol and salt solution. Contaminated, high analysis cost; (2) When the chromatographic retention time of the coexisting components is close to that of sildenafil, it is easy to make a wrong judgment; (3) The instrument has high requirements for the use environment and needs to be placed fixedly, which is not suitable for Cooperation on-site testing with high mobility

[0014] Disadvantages: high analysis cost and complex operation; the instrument has higher requirements on the use environment and needs to be placed in a fixed position, which is not suitable for on-site detection with large mobility, and is not easy to promote and use

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