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A kind of extraction method of pharmaceutical grade bromelain

A technology of bromelain and an extraction method, which is applied in the field of extraction of bromelain, can solve the problems of complex extraction process, high cost, and the content of enzyme bacteria and heavy metals cannot reach pharmaceutical grade standards, and achieves high recovery rate of enzyme activity and reduced energy consumption. The effect of reducing the consumption and shortening the drying time

Active Publication Date: 2014-10-29
四川杨天生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the disadvantages of complex extraction process and high cost in the prior art, and the bacteria content and heavy metal content of the produced enzyme cannot reach the pharmaceutical grade standard, the present invention provides an enzyme activity, bacteria content, heavy metal content, appearance An industrialized extraction method that can meet or exceed pharmaceutical grade standards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The extraction method of pharmaceutical grade bromelain in this embodiment includes the following steps: pineapple raw material pretreatment, coarse crushing, enzymatic hydrolysis, juicing, filtration, addition of auxiliary materials, fine filtration, addition of stabilizer, adsorption by adsorbent, elution, ultrafiltration Filter and freeze-dry to obtain the finished product.

[0039] Specifically: after cleaning the raw materials, place them in an environment of 4°C for pre-cooling for 24 hours, and then pulverize them with a hammer mill; add 0.001% ligninase to the pulverized pineapple pieces, mix them evenly, and transport them to the press through a conveyor belt. There is a feeding chute under the press to collect the pineapple pulp; the pineapple pulp is coarsely filtered by a bag filter, and then filtered by a horizontal filter to obtain pineapple juice with fine residue; After adding 0.2% sodium benzoate in total mass to the pineapple juice and stirring for 15 ...

Embodiment 2

[0041] The extraction method of pharmaceutical grade bromelain in this embodiment includes the following steps: pineapple raw material pretreatment, coarse crushing, enzymatic hydrolysis, juicing, filtration, addition of auxiliary materials, fine filtration, addition of stabilizer, adsorption by adsorbent, elution, ultrafiltration Filter and freeze-dry to obtain the finished product.

[0042] Specifically, the raw materials are cleaned and placed in a 2°C environment for pre-cooling for 20 hours, and then pulverized with a hammer mill; 0.003% ligninase by mass is added to the pulverized pineapple pieces, mixed evenly, and transported to the press by the conveyor belt. There is a feeding chute under the press to collect the pineapple pulp; the pineapple pulp is coarsely filtered by a bag filter, and then filtered by a horizontal filter to obtain pineapple juice with fine residue; After adding 0.5% sodium benzoate in total mass to the pineapple juice and stirring for 10min, a pl...

Embodiment 3

[0044]The extraction method of pharmaceutical grade bromelain in this embodiment comprises the following steps: pineapple raw material pretreatment, coarse crushing, enzymolysis, juice extraction, filtration, adding auxiliary materials, fine filtration, adding stabilizer, adsorbent adsorption, elution, ultrafiltration Filter and freeze-dry to obtain the finished product.

[0045] Specifically: after cleaning the raw materials, place them in a 5°C environment for precooling for 30 hours, and then crush them with a hammer mill; add ligninase with a mass percentage of 0.01% to the crushed pineapple pieces and mix them evenly, and then transport them to the press through a conveyor belt. There is a receiving tank under the squeezer to collect pineapple pulp; the pineapple pulp is coarsely filtered through a bag filter, and then circulated through a horizontal filter to obtain pineapple juice with fine residue; Add the total mass of 0.1% sodium benzoate to the pineapple juice and s...

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Abstract

The invention relates to a method for preparing bromelain, and in particular relates to a method for extracting high-purity pharmaceutical grade bromelain. The method comprises the following steps of: pre-treating pineapple; performing coarse crushing and zymolysis; squeezing a juice; filtering; adding an auxiliary material; performing fine filtration; adding an stabilizing agent; absorbing by using an absorbing agent; and performing elution, ultrafiltration and freeze-drying to obtain a finished product. The method has the advantages that a kaolin adsorption method, a tannin precipitation method and an organic solvent extraction method in the conventional extraction of bromelain are replaced by a cellulose adsorption method, so that the yield is high, the process is simple, the energy consumption is low, and the indexes including activity, bacterial content, heavy metal content, appearance and the like of the produced bromelain reach or exceed a pharmaceutical grade standard. Besides, adsorptive cellulose can be used repeatedly after elution and regeneration, so that the production cost can be greatly reduced.

Description

technical field [0001] The invention relates to a method for extracting bromelain, in particular to a method for extracting pharmaceutical grade bromelain. Background technique [0002] Bromelain is a proteolytic enzyme. Bromelain extracted from pineapple stems is a pure natural plant protease with strong ability to decompose fibrin and blood clots. It is widely used in the pharmaceutical industry to prevent and treat cardiovascular diseases and inhibit the growth of tumor cells. [0003] At present, the extraction methods of bromelain mainly include chemical extraction methods and physical extraction methods. For example, patent CN1908165A provides a new method for physical extraction of bromelain. Juicing-centrifugation-freezing-filtration-ultrafiltration-drying-finished product, the whole process consumes a lot of energy, the production process is complicated and tedious, and the cost is high, which is not conducive to large-scale production, and the yield of the bromela...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50
Inventor 诸辉石亚妮吴吕平
Owner 四川杨天生物药业股份有限公司
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