Method for improving terramycin yield

Abstract

The invention discloses a method for improving terramycin. According to the method, by virtue of increasing the expression of synthetic genes of three enzymes in the cell in a terramycin synthetic route, the formation of tetracycline carbon skeleton which is the precursor of the terramycin synthetic route is promoted, the carbon source flux of the terramycin synthetic route is improved, and thus the yield of the terramycin in streptomyces rimosus is improved.

Description

technical field [0001] The invention belongs to the technical field of streptomyces metabolic engineering, and in particular relates to the method for obtaining a high-yield oxytetracycline strain, constructing a shuttle plasmid and improving the oxytetracycline output of a cultivated mutant strain through optimization of fermentation conditions. Background technique [0002] The method of high-yielding strains of antibiotics in the early days was mainly obtained through the selection of traditional strains, and the high-yielding strains were selected by comparing the spores, colony morphology, pigment production and antibiotic synthesis of different mutant strains. After obtaining some high-yield or low-yield mutant strains, researchers began to conduct in-depth research on the synthesis pathway of antibiotics and the differences between these mutant strains and the original bacteria in terms of DNA level, phenotype, growth, and regulation of metabolic pathways. , use the m...

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Problems solved by technology

However, most of the mutant strains obtained by molecular biology operations reported so far use wild-type strains or standard strains as starting strains. Since the genetic background of these strains is relatively clear, it is easy to perform gene-level operations, but these strains themselves are antibiotics. The output is low, and the output of the obtained mutant strain is far inferior to that of the industrial production strain compared with the mutant strain obtained by the traditional mutagenesis method used in industrial production

However, the use of industrial production strains for molecular biology transformation often does not yield good results. The industrial production strains obtained through multiple mutation breeding have the advantages of short growth cycle and high yield, but their genetic background is relatively complex, and the mutation The site is not clear, and gene knock-in or knock-out on this basis is often interfered by other regulatory genes or complementation pathways on the chromosome, and the production of antibiotics cannot be significantly improved

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