Method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations

A technology for triptolide ketone and triptolide, which is applied in the field of simultaneous determination of triptolide and triptolide, and achieves the effects of good linear relationship, high precision and high degree of separation

Active Publication Date: 2014-12-03
远大医药黄石飞云制药有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there is no report on the simultaneous detection of triptolide and triptolide ketone by microemulsion capillary electrokinetic chromatography (MEEKC)

Method used

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  • Method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations
  • Method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations
  • Method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations

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Embodiment 1

[0031] Embodiment 1 Assay method of the present invention

[0032] The present invention uses Beckman P / ACE TM MDQ capillary electrophoresis instrument (equipped with a diode array detector) was used for method determination. Uncoated fused silica capillary (Beckman company, inner diameter 75 μm, outer diameter 375 μm, total length 61 cm, effective length 50.5 cm).

[0033] 3.1 Composition of Microemulsion

[0034] Surfactant: 1% (w / v) sodium dodecyl sulfate (SDS);

[0035] Co-surfactant: 3% (v / v) n-butanol;

[0036] Oil phase: 1% (v / v) ethyl acetate;

[0037] Water phase: 96% (v / v) 5mmol / L borax-10mmol / L sodium dihydrogen phosphate solution (pH8.5)

[0038] 3.2 Electrophoresis operating conditions

[0039] Separation voltage 25kV, pressure injection 0.5psi×6s, detection wavelength 214nm, temperature 25°C

[0040] 4 specific methods:

[0041] 4.1 Microemulsion preparation

[0042] Mix the components that make up the microemulsion into a stoppered volumetric flask, an...

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Abstract

The invention provides a method for simultaneously detecting triptolide and triptonide in thunder god vine medicinal materials or preparations, which realizes detection by using a micro-emulsion eletrokinetic chromatography method. The method establishes the brand new micro-emulsion eletrokinetic chromatography method, and is used for detecting important toxicity and the content of effective constituents in thunder god vine in a separated manner. Experimental results show the method provided by the invention has the advantages of good linear relation and high precision; and the triptolide and the triptonide in a sample can reach the effect of the base line separation within 10 minutes, and the method is high in separation degree, can be used for detecting an actual sample, and provides powerful supplement for quality control of the thunder god vine medicinal materials or preparations.

Description

technical field [0001] The invention relates to a method for simultaneously determining triptolide and triptolide ketone in triptolide medicinal materials or preparations. Background technique [0002] Tripterygium wilfordii, the root of Euonymus plant Tripterygium wilfordii, is bitter, pungent, cool in nature and highly poisonous. Return liver, kidney channel. Efficacy Expelling wind and dampness, dredging collaterals and relieving pain, reducing swelling and pain, detoxifying and killing insects. It is used for damp-heat nodules and cancerous tumors, and it is used clinically to treat leprosy, rheumatoid arthritis, etc. Pharmacological studies have also shown that it has anti-tumor and anti-inflammatory effects, but it is highly toxic and should be cautious. [0003] Modern research has found that Triptolide and Lactone are contained in Tripterygium wilfordii medicinal materials. Among them, triptolide has good anti-inflammatory and immunosuppressive activities, and is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 余丽双陈立典褚克丹徐伟李煌丛日琳
Owner 远大医药黄石飞云制药有限公司
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