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Preparation method for walnut tannin

A technology of tannins and walnuts, which is applied in the field of preparation of chemical substances in walnut green husks, can solve the problems of high impurities in tannins and low extraction rate of tannins, and achieve high purity of tannins, high extraction rate, and low extraction rate Effect

Inactive Publication Date: 2013-10-09
LULIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a method for preparing walnut tannin in order to solve the problems of low extraction rate of tannin and the extracted tannin containing more impurities in the existing method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of walnut tannin, comprising the following steps:

[0030] Peel off the walnut green skin and dry it in the shade;

[0031] After drying, crush and sieve through a 40 (or 50 or 60 or 70 or 80) mesh screen;

[0032] Put the sifted walnut green peel powder in a container, and add pure water according to the solid-liquid mass ratio of 1:8 (or 9 or 10 or 11 or 12);

[0033] Add pectinase and cellulase with a concentration of 1 (or 1.5 or 2 or 2.5) g / L to the above solution and mix it 1:1, so that the volume ratio of solution and enzyme is 90 (or 95 or 100 or 105 or 110) ): Add in a ratio of 1, adjust the pH value of the solution in the container to 5.5 (or 5.8 or 6 or 6.4 or 6.7), seal the container, and shake it at a constant temperature of 30 (or 35 or 40 or 45 or 50) for 36 (or 42 or 48 or 54 or 60) h;

[0034] Add gelatin with a concentration of 2 (or 3 or 4 or 5)% gelatin at a volume ratio of 10:1 (or 1.3 or 1.5 or 1.7 or 2) to the solution afte...

Embodiment 2

[0040] A preparation method of walnut tannin, comprising the following steps:

[0041] Peel off the walnut green skin and dry it in the shade;

[0042] After drying, crush and sieve through a 40 (or 50 or 60 or 70 or 80) mesh screen;

[0043] Put the sifted walnut green peel powder in a container, and add pure water according to the solid-liquid mass ratio of 1:8 (or 9 or 10 or 11 or 12);

[0044] Add pectinase and cellulase with a concentration of 1 (or 1.5 or 2 or 2.5) g / L to the above solution and mix them at a ratio of 3:1, so that the volume ratio of solution and enzyme is 90 (or 95 or 100 or 105 or 110) ): Add in a ratio of 1, adjust the pH value of the solution in the container to 5.5 (or 5.8 or 6 or 6.4 or 6.7), seal the container, and shake it at a constant temperature of 30 (or 35 or 40 or 45 or 50) for 36 (or 42 or 48 or 54 or 60) h;

[0045]Add gelatin with a concentration of 2 (or 3 or 4 or 5)% gelatin at a volume ratio of 10:1 (or 1.3 or 1.5 or 1.7 or 2) to th...

Embodiment 3

[0052] A preparation method of walnut tannin, comprising the following steps:

[0053] Peel off the walnut green skin and dry it in the shade;

[0054] After drying, crush and sieve through a 40 (or 50 or 60 or 70 or 80) mesh screen;

[0055] Put the sifted walnut green peel powder in a container, and add pure water according to the solid-liquid mass ratio of 1:8 (or 9 or 10 or 11 or 12);

[0056] Add cellulase at a concentration of 1 (or 1.5 or 2 or 2.5) g / L to the above solution at a volume ratio of 90 (or 95 or 100 or 105 or 110):1 of the solution and enzyme, and put the container Adjust the pH value of the medium solution to 5.5 (or 5.8 or 6 or 6.4 or 6.7), seal the container, and shake at a constant temperature of 30 (or 35 or 40 or 45 or 50) ℃ for 36 (or 42 or 48 or 54 or 60) ) h;

[0057] Add acetone with a concentration of 70% (or 75 or 80 or 85 or 90)% to the solution after enzymolysis with a volume ratio of 1:5 (or 6 or 7) in the enzymolysis solution;

[0058] Ce...

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PUM

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Abstract

The invention relates to a preparation method for a chemical substance in walnut green seedcase, and specifically relates to a preparation method for walnut tannin. The preparation method comprises the following steps of striping walnut green seedcases, drying in a shady spot, smashing the walnut green seedcases after drying, screening the smashed walnut green seedcases with a screen mesh; putting the screened walnut green seedcases in a container; preparing a solution; adding pectinase and cellulose with a concentration of 1-2.5 g / L in the above solution; adjusting a pH value of the solution in the container to 5.5-6.7; sealing the container; oscillating for 36-60 h at a constant temperature; adding gelatin with a concentration of 2%-5% in a solution after enzymolysis; standing; filtering to obtain a precipitate; mixing the precipitate with ethyl acetate according to a solid-liquid mass ratio of 1 : 5-8; loading the ethyl acetate solution on a chromatographic column with aluminium oxide powder therein; then adding acetone into the chromatographic column according to a volume ratio of acetone to a solution in the chromatographic column being 3 : 5-9; allowing the solution in the chromatographic column to flow out; and concentrating under a reduced pressure to obtain the tannin. Problems of low extraction rate for the tannin and relatively many impurities in the extracted tannin in a conventional method are overcome.

Description

technical field [0001] The invention relates to a preparation method of chemical substances in walnut green husk, in particular to a preparation method of walnut tannins. Background technique [0002] Walnut is a Juglandaceae plant, and walnut green skin is also called Qinglongyi, which is a thick green peel on the outside of walnut, which is rich in tannin; tannin has astringent properties, and it can be taken orally to treat gastrointestinal bleeding, ulcers and watery diarrhea. Externally applied to wounds and burns, tannin can shrink the microvessels of the wound surface and have local hemostatic effect; tannin also has antibacterial effect and antiviral effect, and tannin can be used as an antidote for alkaloid and certain heavy metal poisoning; Tannins have strong reducing properties, can remove superoxide radicals in organisms, and delay aging; in addition, tannins also have anti-allergic, anti-inflammatory, anthelmintic, and blood pressure-lowering effects. Nowadays...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61K131/00
Inventor 杨卫民杜京旗高晓丽闫艳华赵君刘永青
Owner LULIANG UNIV