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High performance liquid chromatography for detecting quinine sulfate content

A high-performance liquid chromatography, quinine sulfate technology, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of low detection result accuracy, obstacles to accurate detection of quinine sulfate content, and cumbersome operation methods.

Inactive Publication Date: 2013-10-16
ZHANG JIA GANG VINSCE BIO PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current method for determining the content of quinine sulfate, the accuracy of the detection results is low, and the operation method is cumbersome, which has great obstacles to the accurate detection of the content of quinine sulfate.

Method used

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  • High performance liquid chromatography for detecting quinine sulfate content
  • High performance liquid chromatography for detecting quinine sulfate content
  • High performance liquid chromatography for detecting quinine sulfate content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] According to the above-mentioned chromatographic conditions, the quinine sulfate purchased from Malaysia with a calibrated content of 98.0% was assayed.

[0030] Preparation of standard solution: Accurately weigh 25.0mg of standard, place it in a 10ml volumetric flask, add mobile phase and ultrasonic to dissolve the sample and set the volume to the mark.

[0031] Preparation of the sample to be tested: Accurately weigh 25.0 mg of Malaysian quinine sulfate sample, place it in a 10 ml volumetric flask, add mobile phase ultrasound to dissolve the sample and set the volume to the mark.

[0032] Three standard solutions and samples to be tested were prepared respectively, and 5 μl were respectively put into the chromatographic column, and the average value of the obtained peak areas was brought into the above formula for calculation. The calculation results are shown in Table 1.

[0033] Among them, chromatographic column: C18 (4.6 * 250mm5 μ m); mobile phase is methanol-1% ...

Embodiment 2

[0037] According to the above-mentioned chromatographic conditions, the quinine sulfate purchased from India with a calibrated content of 99.4% was assayed.

[0038] Preparation of standard solution: Accurately weigh 25.0mg of standard, place it in a 10ml volumetric flask, add mobile phase and ultrasonic to dissolve the sample and set the volume to the mark.

[0039] Sample preparation to be tested: Accurately weigh 25.0mg of quinine sulfate sample purchased from India, place it in a 10ml volumetric flask, add mobile phase ultrasound to dissolve the sample and set the volume to the mark.

[0040] Three standard solutions and samples to be tested were prepared respectively, and 5 μl were respectively taken into the chromatographic column, and the average value of the obtained peak areas was brought into the above formula for calculation. The calculation results are shown in Table 2.

[0041] Among them, chromatographic column: C18 (4.6 * 250mm5 μ m); mobile phase is methanol-1%...

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Abstract

The invention relates to the drug content detection field, particularly to a high performance liquid chromatography for detecting a quinine sulfate content. And an external standard method is employed to determine the quinine sulfate content. The invention adopts a chromatographic condition of C18(4.6*250mm5micrometers), the mobile phase is composed of methanol and a 1% monopotassium phosphate solution in a ratio of 40:60, and phosphoric acid is taken to adjust the PH of the mobile phase to 4. An ultraviolet detector is used, and the detection wavelength is 316nm. The detection method provided in the invention has the advantages of: good linear relation between a sample concentration and a peak area, good separation effect, high accuracy, and simple and rapid analysis. The method determines that the linear range of quinine sulfate is 0.5 microgram / ml-100 microgram / ml, the linear correlation coefficient r is 1, and RDS is 0.59%.

Description

technical field [0001] The invention relates to a method for determining the content of a medicine, in particular to a high performance liquid chromatography method for detecting the content of quinine sulfate. Background technique [0002] Quinine sulfate (see the attached figure 1 ) is a quinine alkaloid, also known as cinchona alkaloid. Quinine sulfate chemical name: (8S, 9R)-6'-methoxy-cinchona-9-olyl sulfate dihydrate, molecular formula: (C 20 h 24 N 2 o 2 ) 2 ·H 2 SO4·2H 2 O, pure white needle-like crystal, generally dull. Soluble in hot water (1:35) and ethanol (1:125), insoluble in chloroform and ether. Darkens brown on exposure to light. Odorless, with a persistent strong bitter taste. Quinine sulfate has the effects of treating malaria, arrhythmia and anti-inflammation. It can combine with the DNA of Plasmodium to form a complex to inhibit DNA replication and RNA transcription, thereby inhibiting the protein synthesis of the parasite. It is suitable for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张梅彭学东赵金召祁法娟
Owner ZHANG JIA GANG VINSCE BIO PHARM