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Purification method for ramoplanin fermentation liquor

A purification method and fermentation liquid technology, applied in the direction of peptides, etc., can solve the problems of cumbersome operation process, purity impact, unsuitability for industrial production, etc., and achieve the effect of simple operation process, high purification degree and good separation effect

Inactive Publication Date: 2013-10-23
成都开利生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Remoplanin is a biological fermentation product, and it is difficult to purify and separate. The separation and purification methods currently used include macroporous resin adsorption and HPLC separation. The operation of the above two methods is cumbersome and requires high operating skills. Otherwise, it will have a greater impact on the purity
For HPLC separation, the method is expensive and not suitable for industrial production

Method used

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  • Purification method for ramoplanin fermentation liquor
  • Purification method for ramoplanin fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Get the fermented liquid, adjust the pH of the remoplanin fermented liquid to 4, make the fermented liquid precipitate, centrifuge after the precipitation to obtain filter cake and filtrate, add 70% ethanol solution to extract in the filter cake, the quality of its ethanol solution is filter 10 times the mass of the pie. The extract and the filtrate are filtered with an ultrafiltration membrane, the molecular weight cut-off of the ultrafiltration membrane is 10000, and the filtration pressure is 0.1 MPa; the filtrate is collected to obtain liquid A.

[0018] Concentrate liquid A to one tenth of its original volume, adjust its pH to 4, separate by gel chromatography, collect the eluent, and obtain liquid B. The molecular sieve of the gel chromatography uses Sephadex LH-20 dextran gel, which is packed into a column, and the height of the column is 2m.

[0019] In the separation process of gel chromatography, first use a mixture of 50% ethanol solution and 2% sodium chlor...

Embodiment 2

[0023] Get the fermented liquid, adjust the pH of the remoplanin fermented liquid to 5, make the fermented liquid precipitate, centrifuge after the precipitation to obtain filter cake and filtrate, add 70% ethanol solution to extract in the filter cake, the quality of its ethanol solution is filter 10 times the mass of the pie. The extract and the filtrate are filtered with an ultrafiltration membrane, the molecular weight cut-off of the ultrafiltration membrane is 10000, and the filtration pressure is 0.2 MPa; the filtrate is collected to obtain liquid A.

[0024] Concentrate liquid A to one-twentieth of its original volume, adjust its pH to 5, separate by gel chromatography, collect the eluate, and obtain liquid B. The molecular sieve of the gel chromatography uses Sephadex LH-20 dextran gel, which is packed into a column, and the height of the column is 3m.

[0025] In the gel chromatography separation process, first use a mixture of 100% ethanol solution and 5% sodium chl...

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Abstract

The invention relates to the technical field of biological pharmacy, and specifically relates to a purification method for a ramoplanin fermentation liquor. Gel chromatography pre-processing is performed on the ramoplanin fermentation liquor by using a ultrafilter membrane, and macro-molecule substances in the fermentation liquor are removed; and the fermentation liquor is purified through gel chromatography for obtaining of a high-purity ramoplanin finished product. The purification method for the ramoplanin fermentation liquor not only helps to guarantee the total yield of the product, but also helps to substantially improve the purity.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for purifying remoplanin fermented liquid. Background technique [0002] A novel glycopeptide antibiotic, remoplanin, was isolated from the fermentation broth of Actinocystoides. Remoplanin is a novel glycopeptide antibiotic that mainly inhibits the growth of Gram-positive bacteria. The raw material drug is a mixture composed of three components A1, A2 and A3, of which A2 is the main component, accounting for more than 80%. Remoplanin can specifically and rapidly inhibit the biosynthesis of the cell wall of Gram-positive bacteria. This inhibitory effect is different from the antibacterial mechanism of vancomycin and teicoplanin, and it has a good antibacterial effect on a variety of drug-resistant pathogenic bacteria. At present, it is mainly developed for the treatment of gastrointestinal inflammatory diseases such as skin infections caused by Gram+ bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K9/00C07K1/36C07K1/34C07K1/16
Inventor 蔡泽贵安丰
Owner 成都开利生物技术有限公司
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