Water-soluble quantum dot fluorescent nanosphere and preparation method thereof

A fluorescent nanometer and quantum dot technology, applied in the field of detection, can solve the problems of easy fluorescence quenching, influence of detection results, and low fluorescence efficiency, and achieve the effects of good fluorescence and colloidal stability, easy operation, and good dispersion

Inactive Publication Date: 2013-10-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two modification methods have their own advantages, but the obtained fluorescent nanospheres contain only one quantum dot, which has the following disadvantages in application: (1) The small particle size (10-30nm) cannot meet all the needs of in vitro and in vivo diagnostics ; (2) Fluorescence quenching is prone to occur after modification and functionalization, resulting in low fluorescence efficiency; (3) A single quantum dot is prone to fluorescence blinking, which has a huge impact on detection results, especially fluorescence imaging and tracing
The encoded microspheres obtained by doping quantum dots in polypropylene microspheres can wrap multiple quantum dots, but the particle size of polypropylene microspheres is in the micron range, which has limitations in the application of fluorescent probes at the molecular scale

Method used

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  • Water-soluble quantum dot fluorescent nanosphere and preparation method thereof
  • Water-soluble quantum dot fluorescent nanosphere and preparation method thereof
  • Water-soluble quantum dot fluorescent nanosphere and preparation method thereof

Examples

Experimental program
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Embodiment example 1

[0031] 1. Preparation process of amphiphilic polymer. The acrylamide polymer used is: polymethacrylic acid, the alkylamine used is: octylamine, and the coupling agent used is EDC. The molar ratio of raw materials is:

[0032] Polymethacrylic acid:octylamine=1:20

[0033] Octylamine: EDC=1:3

[0034] (1) Dissolve 1 g of polymethacrylic acid in 20 mL of DMSO, and stir at 35° C. for 12 to 24 hours.

[0035] (2) Add 26 uL of octylamine into the solution obtained in step (1), continue to stir for 4 to 6 hours, turn off the heating, and cool down to room temperature.

[0036] (3) Weigh 0.12g of EDC, dissolve it in 10mL of DMSO and add it to the mixture in step (2), and react for 12-16 hours. The obtained mixture was purified by dialysis with ethanol and water respectively, and then taken out and freeze-dried to obtain the amphiphilic polymer.

[0037] 2. The preparation process of water-soluble quantum dot fluorescent nanospheres. The quantum dots used are CdZnSe quantum dots,...

Embodiment example 2

[0044] 1. Preparation process of amphiphilic polymer. The acrylamide polymer used is: polymethacrylic acid; the alkylamine used is: octylamine; the coupling agent used is EDC. The molar ratio of raw materials is:

[0045] Polymethacrylic acid:octylamine=1:40

[0046] Octylamine: EDC=1:3

[0047] (1) Dissolve 1 g of polymethacrylic acid in 20 mL of DMSO, and stir at 35° C. for 12 to 24 hours.

[0048] (2) Add 51 uL of octylamine into the solution obtained in step (1), continue to stir for 4 to 6 hours, turn off the heating, and cool down to room temperature.

[0049] (3) Weigh 0.23g of EDC, dissolve it in 10mL of DMSO and add it to the mixture in step (2), and react for 12-16 hours. The obtained mixture was purified by dialysis with ethanol and water respectively, and then taken out and freeze-dried to obtain the amphiphilic polymer.

[0050] 2. The preparation process of water-soluble quantum dot fluorescent nanospheres. The quantum dots used are CdZnSe quantum dots, the...

Embodiment example 3

[0057] 1. Preparation process of amphiphilic polymer. The acrylamide polymer used is: poly(2-ethylacrylic acid); the alkylamine used is: dodecylamine; the coupling agent used is EDC. The molar ratio of raw materials is:

[0058] Poly(2-ethylacrylic acid): dodecylamine = 1:60

[0059] Octylamine: EDC=1:3

[0060] (1) Dissolve 1 g of poly(2-ethylacrylic acid) in 20 mL of DMSO, and stir at 35°C for 12 to 24 hours.

[0061] (2) Weigh 0.12 g of dodecylamine and add it to the solution obtained in step (1), continue to stir for 4 to 6 hours, turn off the heating, and cool down to room temperature.

[0062] (3) Weigh 0.35g of EDC, dissolve it in 10mL of DMSO and add it to the mixture in step (2), and carry out the reaction for 12-16 hours. The obtained mixture was purified by dialysis with ethanol and water respectively, and then taken out and freeze-dried to obtain the amphiphilic polymer.

[0063] 2. The preparation process of water-soluble quantum dot fluorescent nanospheres. ...

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Abstract

The invention relates to a water-soluble quantum dot fluorescent nanosphere and a preparation method thereof. The preparation method comprises the following steps of: grafting alkylamine on a side chain of a hydrophilic polymer of polyacrylic acids to prepare an amphipathic polymer; and coating the amphipathic polymer on the surface of a quantum dot by using a reaction system of water/an emulsifying agent/the amphipathic polymer/oil through a phase transfer method to obtain a fluorescent nanosphere with the quantum dot coating quantity of 10-80, the uniform grain size of 30-300nm and favorable dispersibility. The water-soluble quantum dot fluorescent nanosphere is good in fluorescence and colloid stability, capable of being stored for a long term, rich in carboxyl on the surface, capable of being coupled with an antibody and other amino-contained biomolecules such as antibodies, nucleic acids, enzymes, amino acids and even amino-contained inorganic molecules, easy in operation of coupling reaction of carboxyl and amino, high in connection efficiency, simple in preparation process and suitable for large-scale production.

Description

technical field [0001] The invention relates to a water-soluble quantum dot fluorescent nanosphere and a preparation method thereof, belonging to the technical field of detection. Background technique [0002] Quantum dots (QDs), which are semiconductor nanocrystals, have received widespread attention as a new type of fluorescent marker due to their unique optical properties. Good color and adjustable color, fluorescence intensity is 20 to 50 times that of traditional organic fluorescein, and has long-lasting photochemical stability. Quantum dots with good photochemical properties are usually synthesized in the oil phase with the existing technology, which cannot be directly applied in the biological environment. Therefore, obtaining quantum dot fluorescent nanospheres with good biocompatibility, easy functionalization, uniform particle size and good stability is of great significance to the development of DNA hybridization, molecular imaging, biosensing, and especially cli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/02C08F120/06C08F8/32G01N33/533
Inventor 常津李雪杨秋花武玉东
Owner TIANJIN UNIV
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