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Material for tissue regeneration, aqueous protein solution containing same, and method for gelling same

A protein aqueous solution and tissue regeneration technology, applied in the fields of peptide/protein components, chemical instruments and methods, animal/human proteins, etc., can solve the problems of bacterial infection, easy proliferation and deterioration of bacteria, and achieve inhibition of bacterial proliferation and granulation tissue formation. or excellent effect of promoting epithelialization

Inactive Publication Date: 2013-10-23
KYOTO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen sponge has the characteristics of good biocompatibility, but it has problems such as susceptibility to bacterial infection, easy proliferation of bacteria, deterioration due to exudate, and difficulty in obtaining materials.

Method used

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  • Material for tissue regeneration, aqueous protein solution containing same, and method for gelling same
  • Material for tissue regeneration, aqueous protein solution containing same, and method for gelling same
  • Material for tissue regeneration, aqueous protein solution containing same, and method for gelling same

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0127] ○Production of SELP8K polymer

[0128] Plasmid pPT0345 encoding SELP8K was prepared according to the method described in the Examples of Japanese Patent No. 4088341.

[0129] The produced plasmid was transformed into Escherichia coli to obtain a SELP8K production strain. A method for producing a SELP8K polymer using a SELP8K producer strain is shown below.

[0130] An overnight culture solution of a SELP8K producer strain grown at 30° C. was used to inoculate 50 ml of LB medium in a 250 ml flask. Kanamycin was added to a final concentration of 50 µg / ml, and the culture solution was cultured at 30°C with stirring (200 rpm). When OD600 of the culture solution was 0.8 (absorbance meter UV1700: manufactured by Shimadzu Corporation), 40 ml was transferred to a flask previously warmed to 42° C., and cultured at the same temperature for about 2 hours. The culture was cooled on ice, and the OD600 of the culture solution was measured. Escherichia coli was collected by centri...

manufacture example 2

[0141] In Production Example 1, except that "plasmid pPT0345 encoding SELP8K" was used instead of "plasmid pPT0345 encoding SELP0K", an artificial protein polymer (A-2) having a molecular weight of about 82 kDa sequence (12) was obtained in the same manner .

manufacture example 3

[0143] In Production Example 1, the artificial protein polymer (A -3).

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Abstract

The purpose of the invention is to provide a material for tissue regeneration, which suppresses bacterial growth and accelerates granulation tissue formation or epithelialization. The invention is a material for tissue regeneration comprising an artificial protein polymer (A), wherein (A) has a polypeptide chain (Y), in which 2 to 200 amino acid sequences (X) of any one type of a VPGVG sequence (2), a GVGVP sequence (3), a GPP sequence, a GAP sequence, and a GAHGPAGPK sequence (4) are continuous, and / or a polypeptide chain (Y') obtained by substituting 1 to 100 amino acids in (Y) with lysine and / or arginine, and the total number of (Y) chains and (Y') chains in (A) is 1 to 100, and the hydrophobicity of (A) is 0.2 to 1.2.

Description

technical field [0001] The present invention relates to a material for tissue regeneration, an aqueous protein solution containing the material for tissue regeneration, and a method for gelling the material for tissue regeneration. Background technique [0002] Gauze, absorbent cotton, and the like have conventionally been used as wound covering materials for protecting and temporarily applying to affected areas caused by diseases or traumas such as burns, peelings, skin peelings, and traumatic skin defects. They can quickly absorb exudate, but on the other hand, they are susceptible to bacterial infection, and if the wound is dry, there are problems such as pain and bleeding during removal. In addition, in order to keep the wound surface from drying out, a wound dressing material is used in combination with an ointment, etc., but this has the problem of insufficient absorption of the exudate and the wound surface becomes excessively wet. [0003] In addition, as a material...

Claims

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Application Information

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IPC IPC(8): A61L27/00C07K5/083C07K7/06C07K14/00C12N15/09
CPCA61L27/227C07K14/78A61K38/00A61L26/0028A61L26/008C07K5/0806
Inventor 铃木茂彦河合胜也川端慎吾
Owner KYOTO UNIV
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