Leaf in-vitro culturing method for miniature ornamental photinia fraseri

A technology of in vitro culture and red leaves, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long multiplication cycle, many explants, and low multiplication coefficient

Active Publication Date: 2013-11-20
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing reproduction technology, the method of tissue culture has been reported, but in the report, the buds of the shoots that were born in the same year are used as the buds of the explants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The basic medium formula used for the in vitro culture of miniature ornamental Photinia fragrans leaves is as table 3, table 4

[0050] Table 3 Contains substance species and quality in every liter of basic medium (induced callus culture)

[0051] Chemical Name

Chinese name

Concentrationmg / L

KNO 3

potassium nitrate

1520

NH 4 NO 3

ammonium nitrate

1320

MgSO 4 ·7H 2 o

Magnesium Sulfate Heptahydrate

248

K H 2 PO 4

Potassium dihydrogen phosphate

136

CaCl 2 2H 2 o

Calcium Chloride Dihydrate

440

MnSO 4 4H 2 o

Manganese Sulfate Tetrahydrate

22.3

ZnSO 4 ·7H 2 o

Zinc sulfate heptahydrate

8.6

h 3 BO 3

boric acid

6.2

CuSO 4 ·5H 2 o

Copper Sulfate Pentahydrate

0.025

CoCl 2 .6H 2 o

Cobalt chloride

0.025

Na 2 MoO 4 2H 2 o

Sodium molybdate dihydrate

0.25

...

Embodiment 2

[0066] This embodiment is operated according to the steps of Example 1, except that the weight ratio of the medium and the raw material components are different. The basic medium formula used in this example is shown in Table 5 and Table 6, and the propagation of proliferation and strong seedlings is cultivated for 35 days. The coefficient is 5.2, the height is 4.3cm, and the rooting rate is 98.3%.

[0067] Containing material type and quality (inducing callus culture) in every liter of basic medium in table 5

[0068] Chemical Name

Chinese name

Concentrationmg / L

KNO 3

potassium nitrate

1900

NH 4 NO 3

ammonium nitrate

1650

MgSO 4 ·7H 2 o

Magnesium Sulfate Heptahydrate

370

K H 2 PO 4

Potassium dihydrogen phosphate

170

CaCl 2 2H 2 o

Calcium Chloride Dihydrate

440

MnSO 4 4H 2 o

Manganese Sulfate Tetrahydrate

22.3

ZnSO 4 ·7H 2 o

Zinc sulf...

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PUM

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Abstract

A leaf in-vitro culturing method for miniature ornamental photinia fraseri comprises: employing an annual tender leaf of perennial miniature ornamental photinia fraseri as an explant, after disinfection and sterilization, inoculating into a callus induction culture medium, placing a dark place for 10-15 days, placing under a common fluorescent lamp, at 20-25 DEG C illuminating for 15 hours every day with humidity of 50%-60% for 48 days, to induct and form a callus; cutting the callus and transplanting to a disinfected differentiation propagation medium for propagation culture; after continuous propagation, cutting the propagated test-tube seedling and transplanting to a disinfected seedling-strengthening culture medium for seedling-strengthening culture; after 30 days removing the basal callus and parts of leaves, leaving 3-4 sheets of leaves, inoculating to a disinfected rooting culture medium for rooting culture for 8-10 days; and taking out and washing, transplanting to a substrate containing peat and yellow subsoil with a volume ratio of 3:2, wherein rooting surviving is realized after 15 days and the rooting rate reaches 96% or more.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant culture tissue, in particular to a tissue culture method in which miniature ornamental Photinia fragrans leaves are used as explants. Background technique [0002] Miniature ornamental Photinia lochengensis Yü is an evergreen shrub or small tree. It has advantages: 1) It has good cold resistance, can withstand the lowest temperature of -20°C, can grow normally in the south of Beijing, and has better cold resistance than other Photinia varieties; The new leaves are all bright red and bright red, and the autumn leaves germinate in late September and turn bright red again. Compared with other Photinia fruticosa varieties, the red leaf period is longer, the color is bright, the branches and leaves are dense and small, and the germination ability is strong. Strong branches, good ornamental effect; 3) Moderate growth rate, but slower than other varieties, can be widely used in color blocks and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 蒋泽平董筱昀吕运舟
Owner JIANGSU ACAD OF FORESTRY
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