Method for somatic embryogenesis and plant regeneration of macleaya cordata

A technology of embryogenesis and somatic cells, which is applied in the field of plant tissue culture, can solve problems such as research reports, and achieve the effects of large reproduction coefficient, pure germplasm, and efficient industrial seedling production

Active Publication Date: 2013-11-27
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant research report at home and abroad on the tissue culture technology of somatic embryogenesis in Boluohui

Method used

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  • Method for somatic embryogenesis and plant regeneration of macleaya cordata
  • Method for somatic embryogenesis and plant regeneration of macleaya cordata
  • Method for somatic embryogenesis and plant regeneration of macleaya cordata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) The preparation of the medium, including the basic medium and the components of the medium in each stage of tissue culture and the weight of each component in each liter of medium:

[0034] 1) Basic medium: Boluohui filament callus induction and proliferation, embryoid body induction, proliferation, and seedlings all adopt MS medium; among them, agar 7g / L, sucrose 30g / L, pH5.8;

[0035] 2) Embryogenic callus induction medium: MS+2,4-D0.5mg / L+6-BA0.5mg / L;

[0036] 3) Somatic embryo induction medium: MS+GA 3 0.5mg / L;

[0037] 4) Somatic embryo maturation induction medium: MS+GA 3 0.5mg / L;

[0038] 5) Somatic embryo germination and seedling growth medium: MS;

[0039] (2) Bo falls back to somatic embryogenesis:

[0040] 1) Extraction and disinfection of explants: Take the unopened Boluohui flower buds, wash them with distilled water, and rinse them with sterile water on an ultra-clean workbench; soak them in 70% alcohol for 10 seconds, and then use 0.1% mercuric c...

Embodiment 2

[0047] This example adopts embryogenic callus induction medium: 2, 4-D is respectively 0.8, 1.0, 1.5, 2.0, 2.5 and 3 mg / L; the rest of the implementation steps are the same as in Example 1, all of which can achieve the effect of Example 1 .

Embodiment 3

[0049] This example uses somatic embryo induction medium: GA 3 Take 0, 0.2, 0.8, 1 mg / L respectively; the rest of the implementation steps are the same as in Example 1; or IAA is 0, 0.1 and 0.25 mg / L respectively, and the rest of the implementation steps are the same as in Example 1; or KT is 0,0.1 And 0.25mg / L, all the other implementation step techniques are the same as embodiment 1, all can reach the effect of embodiment 1.

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Abstract

The invention discloses a method for somatic embryogenesis and plant regeneration of macleaya cordata, which belongs to the field of plant tissue culture techniques. The method comprises the following steps: (1) preparation of a medium; (2) somatic embryogenesis of macleaya cordata; (3) germination and seedling formation of a somatic embryo; and (4) transplantation, culture and the like of a regenerated seedling. According to the invention, the method is a tissue culture method established with filament of macleaya cordata as an explant for induction of embryoid and can realize rapid, high-efficiency and large-scale production of high-quality macleaya cordata seeds and seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for somatic cell embryogenesis and plant regeneration of Boluohui. Background technique [0002] Macleaya cordata (Willd) R. Br belongs to the genus Macleaya cordata (Willd) R. Br of the family Papaveraceae (Papaveraceae), also known as trumpet pole and mountain horn. It is a perennial erect large herb, about 1-4 meters high, with white powder all over, rhizome Thick, green stems, cylindrical hollow and hairless, mainly distributed in the Yangtze River Basin of my country and the provinces south of it. "Supplements to Materia Medica" records that Boluohui is warm, bitter, and poisonous in nature. , "Vitiligo", etc. Boluohui has a long history of application in the folks for the treatment of carbuncles, toxins, malignant sores, ulcers, scabies and other body surface diseases. Modern pharmacological studies have shown that Boluohui contains sanguinarine, cheler...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 曾建国李炎林柳亦松芦强宋锡帅
Owner HUNAN AGRICULTURAL UNIV
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