Compound biological herbicide and application method thereof

A herbicide and biological technology, applied in the directions of biocides, herbicides and algicides, botanical equipment and methods, etc., can solve the problems of complicated and long roads, achieve high crop safety, reduce dosage, overcome humidity the effect of dependence

Active Publication Date: 2013-11-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the main reasons is that it is easy to isolate a potentially useful patho

Method used

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  • Compound biological herbicide and application method thereof
  • Compound biological herbicide and application method thereof
  • Compound biological herbicide and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Compatibility testing

[0023] (1) Cultivation of conidia: inoculate QZ-2000 in a culture medium containing potatoes, glucose and agar at 25 degrees for 3-5 days. Wheat bran medium or rice bran culture is based on atmospheric pressure sterilization for 45-55 minutes. Transfer the well-grown colonies to sterilized wheat bran solid medium or wheat bran-rice bran medium, culture them in a 25-degree incubator for 5-8 days, cut the bacterial blocks into small pieces and spread them on white In the porcelain plate, spray an appropriate amount of sterile water and irradiate it under a black light for 2-3 days, air-dry the medium, place it in a spore separator and run it for 3-5 minutes, roughly separate the spores, and then sieve to obtain relatively pure spores .

[0024] (2) Organic acid solution configuration: take oxalic acid, acetic acid and formic acid respectively to configure 1×10 5 ppm of organic acid mother liquor.

[0025] (3) Accurately weigh 1g of the spores o...

Embodiment 2

[0028] Detached leaf detection

[0029] The chemical herbicide Yu Nong Le 1 / 10 times and spore +1 / 10 Yu Nong Le were used as controls. Take leaves of the same size (third leaf) in the same position (third leaf) of crabgrass at the 4-leaf stage, first sterilize the surface with 75% alcohol, then rinse with sterile water several times, cut into pieces about 5 cm long, and set aside. Treatment method: take 6 leaves for each treatment, gently shake and immerse in 10ml treatment solution for 30 seconds, take out the leaves, flatten them and place them in a petri dish covered with wet filter paper (keep the filter paper moist), and place them in a light culture at 25°C box (light / dark, 12 hours / 12 hours) for 2 days, and then take out and detect the incidence of crabgrass detached leaves, as shown in Table 1-3.

[0030]Table 1 Effects of different oxalic acid concentrations on the pathogenicity of Curvularia sp. QZ-2000 spores (2 days)

[0031]

[0032] Note: Different lowercase...

Embodiment 3

[0041] Compatibility testing of organic acids and Alternaria spores

[0042] Prepare a compound solution of oxalic acid, acetic acid, formic acid and spores with different concentration gradients, spread it on a 1% water agar plate, and observe the germination rate of spores after 5 hours. It was found that when the concentration of oxalic acid was below 40000ppm, the germination rate of spores was greater than 75%. When the concentration of acetic acid and formic acid is below 8000ppm, the spore germination rate is greater than 55%. The spore germination rate test shows that the spores have good compatibility with oxalic acid, acetic acid and formic acid, and can be used in combination.

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Abstract

The invention discloses a compound biological herbicide and an application method thereof, belonging to a compound application technology of a biological herbicide and an organic acid. The biological herbicide comprises used spore, mycelia and metabolite types; the organic acid is selected from an oxalic acid, an acetic acid or a formic acid; the mass ratio of the biological herbicide to the organic acid is (1:0.02)-(1:4). When the compound biological herbicide disclosed by the invention is used, the biological herbicide and the organic acid can be prepared into a water solution to be sprayed, and also can be sprayed separately. By using the compound ratio of the compound biological herbicide to the organic acid, the compatibility is good, the organic acid has a synergistic effect on the biological herbicide, and the environment-friendly effect is achieved.

Description

technical field [0001] The invention relates to a compound herbicide, in particular to a herbicide compounded with a biological herbicide and an organic acid and a use method thereof. Background technique [0002] Weeds are considered to be one of the main enemies that seriously damage crops. There are 30,000 kinds of weeds widely distributed in the world, and about 1,800 kinds of weeds cause different degrees of damage to crops every year, and the annual crop yield reduction due to weed damage is as high as 9.7%. According to statistics, the area affected by weeds in my country in 2002 exceeded 1.133 billion mu, including 317 million mu of rice, 283 million mu of wheat, 186 million mu of corn, 71.7 million mu of soybean, 149 million mu of cotton, sugar, oil, etc. 225 million mu of crops. In 2003, the planting area of ​​crops (excluding fruit tea) in my country was 2.087 billion mu, the area of ​​farmland weed damage was 1.114 billion mu, and the area of ​​serious damage was...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P13/00
Inventor 强胜卢玉霞陈世国魏冉王玲庄超匡静周兵戴伟民宋小玲
Owner NANJING AGRICULTURAL UNIVERSITY
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