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Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive

The technology of extract and cassia twig, applied in the field of cinnamon twig extract and preparation thereof, can solve the problems of no cassia twig inhibiting citrus postharvest pathogens and the like, and achieve the effects of shortening extraction cost, low extraction temperature and remarkable bacteriostatic effect.

Active Publication Date: 2013-11-27
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the antibacterial activity of cassia twig, only Han Aixia et al. studied the antibacterial effect of cassia twig decoction extract on Staphylococcus aureus, Staphylococcus albus, Pseudomonas aeruginosa, Proteus, Streptococcus A, and Streptococcus B. See the systematic research report on the inhibition of citrus postharvest pathogens by Guizhi

Method used

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  • Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive
  • Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive
  • Preparation method of cassia twig extractive, prepared cassia twig extractive and application of prepared cassia twig extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: a kind of cassia twig extract

[0050] Prepared by the following steps:

[0051] 1. 40 DEG C of drying 5kg cassia twigs, after crushing, pass through a 40-mesh sieve, and get the powder under the sieve;

[0052] II. the powder that step I obtains adds the ethanol that 10 times of weight percent concentration of described cassia twig weight is 95% to carry out ultrasonic-assisted extraction, and extraction temperature is 40 ℃, and extraction time is 2 hours, and ultrasonic frequency is 30 KHz; Filter, Obtain alcohol extract;

[0053] III. The alcohol extract obtained in step II, the ethanol was recovered under reduced pressure until there was no alcohol smell, and 462g of the target product (cinnamon radix alcohol extract) was obtained.

[0054]

Embodiment 2

[0055] Example 2 A kind of cassia twig extract

[0056] Prepared by the following steps:

[0057] 1. 5kg cassia twigs are dried at 50°C, crushed and passed through a 40-mesh sieve, and the powder under the sieve is taken;

[0058] II. the powder that step I obtains is added the ethanol that 20 times of weight percentage concentration of described cassia twig weight is 90% and carries out ultrasonic-assisted extraction, and extraction temperature is 50 ℃, and extraction time is 2 hours, and ultrasonic frequency is 30 KHz; Filter, Obtain alcohol extract;

[0059] III. The alcohol extract obtained in step II, the ethanol was recovered under reduced pressure until there was no alcohol smell, and 469g of the target product (cinnamon radix alcohol extract) was obtained.

[0060]

Embodiment 3

[0061] Example 3 A kind of cassia twig extract

[0062] Prepared by the following steps:

[0063] 1. 5kg cassia twigs are dried at 50°C, crushed and passed through a 40-mesh sieve, and the powder under the sieve is taken;

[0064] II. In the powder that step I obtains, add the ethanol that the weight percent concentration of 30 times of described cassia twig weight is 80% carries out ultrasonic-assisted extraction, and extraction temperature is 60 ℃, and extraction time is 3 hours, and ultrasonic frequency is 40 KHz; Filter, Obtain alcohol extract;

[0065] III. The alcohol extract obtained in step II, the ethanol was recovered under reduced pressure until there was no alcohol smell, and 475g of the target product (cinnamon radix alcohol extract) was obtained.

[0066]

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PUM

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Abstract

The invention provides a preparation method of a cassia twig extractive. According to the preparation method, ethanol is used to extract cassia twigs under the support of ultrasonic waves. The preparation method can further comprise ethyl acetate extraction and silica gel column purification. The invention further provides the cassia twig extractive prepared by the preparation method provided by the invention, and an application of the cassia twig extractive to preparation of a medical composition for inhibiting citrus postharvest pathogens. The cassia twig extractive has the stronger inhibition effect on the citrus postharvest pathogens, has no chemical residues after being used for keeping the freshness of citruses, has no damages to the health of a human body, has no pollution to the environment and hardly generates drug resistance.

Description

technical field [0001] The invention belongs to the field of fruit disease prevention and preservation, and in particular relates to a cassia twig extract capable of inhibiting postharvest pathogenic bacteria of citrus, a preparation method and application thereof. Background technique [0002] Citrus is the largest fruit in the world. At present, the planting area and output of citrus in my country ranks first in the world, and it occupies a very important position in my country's agricultural economy. [0003] The citrus fruit tissue is tender and juicy and rich in sugar. It is prone to mechanical damage in various links such as picking, packaging, storage, transportation and sales. After the peel is damaged, it is vulnerable to the attack of various pathogenic bacteria and postharvest diseases. These diseases are easy to cause fruit to rot and deteriorate. The rot rate is generally 10% to 30%, and can reach more than 40% to 50% in severe cases, causing huge economic losse...

Claims

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Application Information

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IPC IPC(8): A01N65/24A01P3/00A23B7/154
Inventor 陈金印万春鹏周梦娇
Owner JIANGXI AGRICULTURAL UNIVERSITY
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