Screening method of fusarium antagonistic bacteria

A screening method and technology for Fusarium, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of cumbersome screening process and low efficiency of antagonistic bacteria, saving manpower, ensuring correctness, and saving culture medium Effect

Inactive Publication Date: 2013-11-27
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a fast, simple and efficient screening method for Fusarium antagonistic bacteria aiming at the cumbersome and inefficient screening process of antagonistic bacteria.

Method used

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  • Screening method of fusarium antagonistic bacteria
  • Screening method of fusarium antagonistic bacteria
  • Screening method of fusarium antagonistic bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Culture medium of the present invention:

[0023] LB liquid medium: peptone 10.0 g / L, yeast extract 5.0 g / L, NaCl 2.0 g / L (pH 7.0~7.5), then add agar 15~20 g / L;

[0024] PDA liquid medium: potato 200 g / L, glucose 20 g / L, then add agar 15~20 g / L;

[0025] Mung bean soup medium: 30 g of mung beans, boiled with distilled water until about 50% of the mung beans flowered, filtered with sterile gauze, and the filtered supernatant was adjusted to 1L.

Embodiment 2

[0027] Collect the soil of the corn root system, put it into sterile water and mix it evenly, and prepare 10 -5 ,10 -7 ,10 -9 Spread the dilutions of different dilutions on the LB and PDA plates respectively, repeat each dilution twice, place the coated culture dishes at 28°C for more than 48 hours, pick and coat Plates for cultivating bacteria are ready for use.

[0028] After activation of Fusarium graminearum GZ3639 (the strain was donated by the Mycotoxin Research Center of the United States Department of Agriculture) on PDA medium, it was inserted into the mung bean soup medium, cultivated at 25°C for one week, and then filtered through sterile gauze to form the sickle Bacteria spore liquid; put the Fusarium spore liquid into a sterile watering can, quickly spray inoculate against the plate with bacteria, place it upright for 1 hour, then place it upside down in a 28°C incubator for 48 hours, and observe the plate. If there is a zone of inhibition around the bacteria, ...

Embodiment 3

[0036] Collect wheat ears, grind them in a mortar, shake and mix them in sterile water, and prepare 10 -3 ,10 -5 ,10 -7 Spread the dilutions of different dilutions on the LB and PDA plates respectively, repeat each dilution twice, place the coated Petri dishes at 28°C for 48 hours, pick and culture The flat plate with bacteria is ready for use;

[0037] After activation of Fusarium graminearum GZ3639 (the strain was donated by the Mycotoxin Research Center of the United States Department of Agriculture) on PDA medium, it was inserted into mung bean soup medium, cultured at 25°C for one week, and then filtered through sterile gauze to form the sickle Bacteria spore liquid; put the Fusarium spore liquid into a sterile watering can, quickly spray inoculate against the plate with bacteria, place it upright for 1 hour, then place it upside down in a 28°C incubator for 48 hours, and observe the plate. If there is a zone of inhibition around the bacteria, it indicates that the bac...

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Abstract

The invention relates to a fast and efficient screening method of fusarium antagonistic bacteria. The screening method adopts a flat plate-based two-step culture technology and comprises the following steps of separating bacteria in a nutrient medium, spraying a fusarium spore liquid on a flat plate with the bacteria, carrying out culture at a temperature of 28 DEG C, and observing a bacterium inhibition zone around the bacteria, wherein if the bacterium inhibition zone exists around the bacteria, it is proved that the bacteria have a fusarium antagonism capacity. Through the one-flat plate two-step culture technology, nutrient medium-based purification of the bacteria separated from a sample is avoided before culture determination in another medium so that the mediums needed in the experiment are greatly reduced, labor, material resources and a cost are reduced and antagonistic bacterium screening efficiency is improved.

Description

technical field [0001] The invention relates to a fast and efficient screening method for biocontrol bacteria, in particular to a fast and efficient screening method for fusarium antagonistic bacteria. Background technique [0002] Plant fungal diseases are an important disease affecting plant growth and development, many of which are caused by Fusarium fungi. Fusarium is a large genus of fungi, widely distributed in nature, and lives parasitic or saprophytic. It can infect the vascular system of a variety of plants (economic crops, medicinal plants and ornamental plants), and produce a variety of mycotoxins in the process of growth and metabolism to endanger crop growth, causing plant root rot, stem rot, flower rot and Ear rot and other diseases seriously affect the yield and quality of crops. For example, Fusarium graminearum causes wheat head blight, not only can lead to a sharp drop in grain production, but also the toxin it produces is a serious hazard to human and an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 徐剑宏史建荣胡晓丹祭芳
Owner JIANGSU ACAD OF AGRI SCI
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