A Fast and Efficient Method for Staining Arbuscular Mycorrhizal Fungi

An arbuscular mycorrhizal fungus, an efficient technology, applied in the field of applied microbiology, can solve the problems of high definition and high contrast, and achieve the effect of enhancing the hierarchy, shortening the digestion time, and improving the transparency effect.

Inactive Publication Date: 2017-02-08
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has high definition, high contrast, and clear arbuscular structure, but Chlorazole Black E is considered to be a suspected carcinogenic substance and has deficiencies

Method used

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  • A Fast and Efficient Method for Staining Arbuscular Mycorrhizal Fungi
  • A Fast and Efficient Method for Staining Arbuscular Mycorrhizal Fungi
  • A Fast and Efficient Method for Staining Arbuscular Mycorrhizal Fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Step 1. Collection and fixation of plant root samples

[0037]In mid-August 2012, 10 young root segments of 5-10 cm from four plants were collected on the campus of Jiangsu University, put into a triangular flask filled with FAA fixative solution, and brought back to the laboratory. According to the characteristics of mycorrhizal infection, avoid taking old roots with severe lignification.

[0038] Step 2. Root sample cleaning and digestion

[0039] The root samples in the FAA fixative solution were taken out and rinsed several times with distilled water to clean the soil on the root surface. Put the washed root samples into four conical flasks filled with 100 ml 10% (W / V) KOH solution, and mark them separately. The four triangular flasks were divided into two groups, among which the roots of Euonymus and Solidago canadensis were darker and classified into group A; the roots of sorrel and foxtail were lighter in color and were classified into group B.

[0040] The ro...

Embodiment 2

[0053] Step 1. Collection and fixation of plant root samples

[0054] In mid-August 2012, 10 young root segments of 5-10 cm from four plants were collected on the campus of Jiangsu University, put into a triangular flask filled with FAA fixative solution, and brought back to the laboratory. According to the characteristics of mycorrhizal infection, avoid taking old roots with severe lignification.

[0055] Step 2. Root sample cleaning and digestion

[0056] The root samples in the FAA fixative solution were taken out and rinsed several times with distilled water to clean the soil on the root surface. Put the washed root samples into four conical flasks filled with 100 ml 10% (W / V) KOH solution, and mark them separately. The four triangular flasks were divided into two groups, among which the roots of Euonymus and Solidago canadensis were darker and classified into group A; the roots of sorrel and foxtail were lighter in color and were classified into group B.

[0057] The r...

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Abstract

The invention relates to a method for rapidly and efficiently dyeing arbuscular mycorrhizal fungi, belonging to the field of application microbiology, and being capable of rapidly and efficiently dyeing and observing the arbuscular mycorrhizal fungi at the root of a plant. The method comprises the following steps of: firstly, carrying out transparency treatment on a root tissue by using a KOH (Potassium Hydroxide) solution with the concentration being 20% at high temperature, whitening the root tissue by using H2O2, subsequently dyeing by using trypan blue with the concentration being 0.1%, and observing the structure of the arbuscular mycorrhizal fungi after decoloration by using a decoloration liquid. The method has the characteristics of convenience, rapidness and efficiency, and has the characteristics that the decoloration is thorough, the dyeing effect is clear, the arbuscular structure and the vesicle structure are clear and discernable, and the coloring is long-lasting. The roots of most plants can be dyed by using the method, the arbuscule and the vesicle of the arbuscular mycorrhizal fungi are clearly observed, and the method has good application prospects as the mycorrhiza research is gradually taken seriously.

Description

technical field [0001] The invention relates to a method for quickly and efficiently staining and observing arbuscular mycorrhizal fungi at plant roots, and belongs to the field of applied microbiology. Background technique [0002] Mycorrhizae are symbionts of certain fungi and plant roots in the soil. Arbuscular mycorrhizal fungus (Arbuscular mycorrhizal fungus, AMF) is a kind of soil microorganism widely distributed in nature, it can form a mutualistic symbiosis with the roots of about 90% of terrestrial vascular plants - arbuscular mycorrhizal. The role of arbuscular mycorrhiza is mainly to expand the absorption area of ​​the root system and enhance the absorption of elements (mainly phosphorus) outside the scope of the original root hairs. Mycelium communicates with plant tissues. On the one hand, it absorbs sugar and other organic substances from the host plant as its own nutrition, and on the other hand, it absorbs nutrients and water from the soil to supply plants. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 司春灿林英刘雪艳肖翔杜道林
Owner JIANGSU UNIV
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