3results about How to "Efficient Dyeing" patented technology

The invention discloses a compound dye for fluorescent staining of cells in a complex matrix. The formula of the compound dye comprises a buffer system with the pH value of 6.0-8.0, a dye solution with the pH value of 6.0-8.0 and a dye solution with the total volume of 100 mL, the fluorescent dye is Sybr Gold 2 * (40 *) or 2 to 80 mg of propidium iodide; and 0.1 to 10 mL of triton X-100, and 0.1 to 10 mL of a 0 to 24 mL of glycerol; 0 to 800 mg of EDTA (Ethylene Diamine Tetraacetic Acid)-disodium; the invention relates to a polyamide-amine dendritic polymer. According to the two preferable kit compound fluorescent dye formulas, efficient dyeing of cells in a complex matrix and accurate counting of the number of the cells can be achieved, and the dye has excellent fluorescence developing capacity. Compared with other dyes, the compound dye has the advantages of high dyeing rate (dyeing can be carried out within 30 seconds), stable dyeing, long-time illumination and difficulty in fluorescence quenching.

The invention discloses a non-degreasing biological tissue dyeing method and device. The dyeing method of the non-degreasing biological tissue comprises the following steps: a sample fixing step: fixing a biological tissue sample by adopting paraformaldehyde and / or glutaraldehyde; and a dyeing step: carrying out electrophoresis dyeing on the fixed biological tissue sample which is not degreased by using a nucleic acid dye and a beta-amyloid protein small molecule dye. According to the non-degreasing biological tissue dyeing method disclosed by the invention, the non-degreasing biological tissue is directly dyed, so that the damage of degreasing operation to a sample is avoided; moreover, the nucleic acid dye is combined with the beta-amyloid protein small molecule dye, so that the whole brain sample can be quickly and efficiently dyed, and high-resolution fluorescence labeling and three-dimensional space distribution imaging of beta-amyloid protein plaques in the whole brain range can be realized; the method is of great significance in revealing the spatial distribution pattern of beta-amyloid protein and explaining the pathogenesis of Alzheimer's disease.

The present application relates to a kind of knitted fabric energy-saving dyeing machine and energy-saving dyeing process thereof, it is related to dyeing and finishing technical field, including cloth system, cloth system, dye liquor circulation system, dye vat, bubble removal system and spray head, dye vat includes intercommunication spray pipe, horizontal tank body and inclined tank body, bubble removal system includes bubble removal cylinder, bubble removal pressurizing device and relies on roller group, bubble removal cylinder is at the bottom of inclined tank body, relies on roller group is rotatably installed in bubble removal cylinder, bubble removal pressurizing device is installed in bubble removal cylinder, bubble removal pressurizing device and relies on roller group mutually cooperate, cloth system is at the top of inclined tank body, the top of inclined tank body is equipped with hatch, spray pipe is installed at the bottom of dye vat, the two ends of spray pipe are connected with horizontal tank body and the bottom of bubble removal cylinder respectively, spray head is installed at the end of spray pipe close to bubble removal cylinder, the two ends of dye liquor circulation system are connected with spray head and the bottom of horizontal tank body, cloth system is installed on inclined tank body, bubble removal pressurizing device extrudes to the direction of relying on roller group, so that dyeing is sufficient, reduce energy consumption.