A new rice grain width gene qgs5dl and its molecular marker method
A molecular marker and rice technology, applied in the fields of rice high-yield breeding and molecular genetics, to achieve the effect of facilitating hybridization and speeding up the breeding process
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[0019] The present invention will be further explained below in conjunction with specific implementation examples. The methods used therein are conventional methods unless otherwise specified.
[0020] (1) Single-label localization of new grain width genes
[0021] 1. Test materials
[0022] A hybrid combination of Nongken 58, a popular japonica rice variety introduced from Japan and large-grain mutants from Nongken 58 screened by space mutagenesis, was used to construct a hybrid combination. The first hybrid seed was harvested to form 281 individuals of F 2 Generation segregated groups.
[0023] 2. DNA extraction, PCR amplification and gel electrophoresis
[0024] Refer to the DNA extraction method of Temnykh et al. (2000) to extract genomic DNA from each individual plant. Select 46 SSR markers with polymorphism between the parents to synthesize primers. The genomic DNA of each individual strain is used as a template to perform a polymerase chain (PCR) reaction. The products of the...
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