A new rice grain width gene qgs5dl and its molecular marker method

Abstract

The invention relates to a novel gene qGS5dl capable of increasing rice grain width, grain thickness and grain weight at the same time and a molecular marking method of the novel gene qGS5dl, and belongs to the field of rice high yield and high quality breeding and molecular genetics. The essence of the molecular marking method is that according to the chain separation law, a 'large grain mutant', an F2 population of original parent agricultural reclamation 58 and an F3 family derivative from the F2 population are utilized for positioning the grain length novel gene qGS5dl on a fifth chromosome through single mark analysis so as to obtain a practical and economical mark MGW5 which is closely linked with the grain length novel gene qGS5dl and based on PCR amplification. When the novel gene qGS5dl and the molecular marking method are applied to assistant selective breeding and pyramiding breeding of exterior quality and output of rice, the shortcomings of previous natural variation genes can be effectively made up for, a low-generation breeding population can be subjected to genotype selection in a seedling stage to obtain large grains, timely hybridization and transformation are facilitated, the process of grain authentication in an adult-plant stage is eliminated, breeding efficiency is improved, and the breeding process is accelerated.

Description

technical field [0001] The invention relates to a new rice grain width gene qGS5 dl And its molecular marker, the gene increases rice grain width, increases grain thickness and grain weight at the same time, belongs to the field of rice high-yield breeding and molecular genetics, and is suitable for introducing a new grain shape gene qGS5 in rice high-yield breeding dl , and use molecular markers for assisted selection breeding of the gene. Background technique [0002] Rice grain shape mainly depends on grain length, grain width and grain thickness, and is directly positively correlated with grain weight, one of the yield components. Molecular genetic studies have shown that rice grain shape and grain weight and their components are quantitative traits controlled by multiple genes. So far, more than 110 major QTLs affecting rice grain shape, grain weight and their component traits have been identified, mainly distributed on chromosomes 1, 2, 3 and 5, among which the ones ...

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Problems solved by technology

After comparison and mapping, it was found that there were no reports of genes controlling grain width, grain thickness and grain weight at this locus, so qGS5 dl Possibly a new gene controlling grain width, grain thickness and grain weight

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