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Black barley seed husk flavonoids and its preparation method and application

A technology of flavonoids and highland barley seeds, which is applied to the extraction of flavonoids, the main active components in the bark of black barley seeds, and the application field of preparing medicines for preventing and treating hypoxic injury, can solve the problem that the method for extracting flavonoids from barley barley seed bark has not yet been obtained. and its application, unsuitable for industrial production, and difficult to separate extracts, etc., to achieve the effect of cutting off brain hypoxia protection, good extraction effect, and reduced brain energy consumption.

Active Publication Date: 2016-04-13
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this research needs to crush the black highland barley grains, the process is complicated and affects the extraction rate, and it only stays at the level of anthocyanin extraction, which is only suitable for rough extraction, and the extract is difficult to separate, so it is not suitable for industrial production
So far, there is no relevant report on the extraction method and application of flavonoids from black highland barley seed husk

Method used

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  • Black barley seed husk flavonoids and its preparation method and application
  • Black barley seed husk flavonoids and its preparation method and application
  • Black barley seed husk flavonoids and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0092] Embodiment, obtain black highland barley seed bark extract

[0093] The method for obtaining the black barley seed bark extract comprises the following steps:

[0094] 1) Add granular un-crushed black highland barley skin (directly use the skin of original black highland barley) to leaching in ethanol with a concentration of 20%-60% (volume percentage concentration), the mass / The volume ratio is 1:6-10 (according to the traditional extraction experience, less than 1:6 is insufficient for extraction, and greater than 1:10 will increase the production cost), adjust the pH value to 2.0-5.0, and bathe at 40-60°C for 2-5h.

[0095] The preferred extraction conditions are ethanol concentration 40%, pH 2.0, water bath temperature 50°C, water bath time 3h, to obtain the alcohol extract of flavonoid crude extract;

[0096] 2) Use 101 flocculant (purchased from Beijing Waterly Source Environmental Protection Technology Co., Ltd.) to flocculate and purify the alcohol extract of ...

experiment example 1

[0105] Experimental example 1, black highland barley seed skin extract (flavonoids) prolongs the survival time of mice resistant to hypoxia under normal pressure

[0106] Kunming mice, all male, 30, 18-22g, 10 in each group, the control group was given normal saline by intragastric administration for 3 days, the volume was 0.2mL, the middle dose group was 1.75g ​​black highland barley seed bark extract / kg( body weight), a high dose of 3.5g black barley seed bark extract / kg (body weight), and the black barley seed bark extract is made into a final concentration of 35g / mL. Atmospheric hypoxia tolerance test was carried out 1 hour after the last gavage.

[0107] Specific experimental method: put the mouse into a wide-mouth grinding bottle and seal it, put 10g of soda lime in the bottle, absorb carbon dioxide and water, pad filter paper to absorb urine, record the survival time of the mouse, and take the respiratory arrest as the index.

[0108] The experimental results are shown...

experiment example 2

[0112] Experimental Example 2, Black Highland Barley Seed Bark Extract (Flavonoids) Prolongs the Survival Time of Acute Cerebral Ischemia in Mice

[0113] Kunming mice, weighing 18-22g, were randomly divided into 2 groups, 10 in each group. The control group was intraperitoneally injected with normal saline 0.02mL / g, and the experimental group was injected with 2.0g black highland barley seed bark extract / kg (body weight), the final concentration of the black barley seed bark extract is 35g / mL. After 10 minutes, no anesthesia was needed, the head was quickly decapitated under the ear with large scissors, the gasping time was recorded and t-test was performed.

[0114] The experimental results are shown in Table 6 and Figure 6A , Figure 6B As shown, in the decapitation experiment, the blood supply to the brain is interrupted, but the original blood and nutrients in the brain can still maintain the brain function for a period of time. No effect, indicating that the black b...

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Abstract

The invention discloses highland barley seed peel flavone, and a preparation method and application thereof and belongs to the field of extraction of effective components of plants. By adopting the preparation method, the uncrushed highland barley seed peel is processed by the steps of ethanol crude extraction, flocculant purification, ethyl acetate extraction and enrichment, so as to obtain the highland barley seed peel flavone. Experiments prove that the highland barley seed peel flavone has the effects of preventing and treating hypoxia injury, especially heart and brain hypoxia injuries, can replace rhodiola rosea which is in shortage to prepare a drug for preventing and treating the hypoxia injury, and has a broad industrial application prospect.

Description

technical field [0001] The invention belongs to the extraction process of plant active ingredients, in particular to a method for extracting flavonoids, the main active ingredient in black highland barley seed skin, and its use in the preparation of medicines for preventing and treating hypoxic damage (especially heart and brain hypoxic damage) Applications. Background technique [0002] Highland barley (Hordeum vulgaresp.vulgare), also known as naked barley, is a crop of the genus Barley in the family Poaceae, and has been used as a major food crop in many countries and regions in the world for thousands of years. In Tibet, the resources of highland barley varieties are very rich. There are as many as a dozen varieties of different colors and shapes. Sometimes 7-8 highland barley varieties can be found in the same area. Highland barley is divided into white highland barley, black highland barley, and dark green highland barley. Among them, black highland barley is a unique...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/899A61P39/00A61P9/00A61P9/04A61P11/00A61P9/10A61K131/00
Inventor 于群王璇琳贺敏李伟静任素萍苏丽华丁雪洁乔志新
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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