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Application of biosensing test paper based on N, N'-bis (trimethoxy silicyl propyl)-glutarimide

A trimethoxysilylpropyl, biosensing technology, applied in the field of biosensors, to achieve the effects of high sensitivity, obvious color, good response and linear range

Inactive Publication Date: 2013-12-11
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection of hydrogen peroxide, glucose or prostate-specific antigen based on color-changing biosensing test strips has not been reported

Method used

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  • Application of biosensing test paper based on N, N'-bis (trimethoxy silicyl propyl)-glutarimide
  • Application of biosensing test paper based on N, N'-bis (trimethoxy silicyl propyl)-glutarimide
  • Application of biosensing test paper based on N, N'-bis (trimethoxy silicyl propyl)-glutarimide

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The manufacturing steps of the novel N,N'-bis(trimethoxysilylpropyl)-pentamethylenediimide biosensing test paper are as follows: the filter paper is made into a plurality of small discs with a diameter of 6mm by a puncher, and the Soak in 5vol% APTS ethanol solution for 2 minutes, take it out and let it dry naturally at room temperature, then drip 15μL of 0.125vol% GA aqueous solution on each small disc, appear brick red, dry under 500W infrared lamp for 12 minutes, and Get the brick red biosensing strips.

[0027]Extract the brick red compound on the test paper with a solvent, and pass the infrared test such as figure 1 shown, compared figure 2 The APTS raw material spectrogram, it is obvious from the figure that 1654cm -1 It is an obvious characteristic absorption peak of carbon-nitrogen double bond, figure 1 middle relative figure 2 3349cm -1 and 3282cm -1 The double peak of the amino group disappeared obviously, indicating that APTS and GA generated N,N'-bis...

Embodiment 2

[0029] The biosensing test paper based on N,N'-bis(trimethoxysilylpropyl)-pentamethylenediimine of the present invention is to H 2 o 2 Detection method: Equipped with different concentrations of H 2 o 2 (0, 2.5, 5, 10, 30, 50, 70, 100, 300, 500, 1000mM), take 10μL drops on each biosensing test paper, after 5 minutes, put them in an oven at 130℃, After 10 minutes, the color of each biosensing test paper was photographed respectively, and the color of the biosensing test paper was analyzed by ImageJ2x, and colorimetric analysis was carried out. The standard curve obtained was as follows: image 3 mentioned.

Embodiment 3

[0031] The method for detecting glucose by the biosensing test paper based on N,N'-bis(trimethoxysilylpropyl)-pentamethylenediimide of the present invention: First, drop 10 μL of 15 mg / mL glucose oxidase solution, 10 μL 0.5vol% chitosan solution, equipped with different concentrations of glucose solution (0.25, 0.5, 0.75, 1.0, 2.5, 5.0, 7.5, 10, 15, 20, 30, 60, 80, 100 , 500, 1000mM) were added dropwise to each biosensing test paper, and then reacted in a water bath at 37°C for 10 minutes, and then in an oven at 130°C. After 10 minutes at constant temperature, the color of each biosensing test paper was photographed , with the help of ImageJ2x to analyze the gray value of the biosensing color, and perform colorimetric analysis, the prepared standard curve is as follows Figure 4 shown.

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Abstract

The invention discloses the application of biosensing test paper based on N, N'-bis (trimethoxy silicyl propyl)-glutarimide. The biosensing test paper taking N, N'-bis (trimethoxy silicyl propyl)-glutarimide as a color probe is applied to the detection of hydrogen peroxide, glucose or prostate-specific antigen, has special metachromatism to hydrogen peroxide, and has the advantages of high stability and sensitivity, good selectivity; the detection method is fast, simple and economic, and is wide in application range..

Description

technical field [0001] The invention discloses the application of a biosensing test paper based on N,N'-bis(trimethoxysilylpropyl)-pentamethylenediimide, belonging to the field of biosensors. Background technique [0002] Schiff base mainly refers to a class of organic compounds containing imine or methylimine characteristic groups (-RC=N-), usually Schiff bases are formed by condensation of amines and active carbonyl groups. It has a wide range of applications and can be used in medicine, catalysis, analytical chemistry, corrosion and other fields. In the field of medicine, Schiff bases have biological activities of antibacterial, bactericidal, antitumor, and antiviral; in the field of catalysis, cobalt and nickel complexes of Schiff bases have been used as catalysts; in the field of analysis, Schiff bases are used as good Ligands can be used to identify and identify metal ions and quantitatively analyze the content of metal ions; in the field of corrosion, some aromatic S...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/78
Inventor 阳明辉周苗周飞艨
Owner CENT SOUTH UNIV
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