A kind of suspending agent compounded by Bacillus thuringiensis and I. fumigatus and its preparation method

A technology of I. fumoscens and Bacillus thuringiensis, which is applied to botany equipment and methods, insecticides, biocides, etc., can solve the problems of reducing the effect, affecting the persistence of effect, and inactivating spores, so as to improve the Effect of prevention, improvement of quick-acting performance, and effect of prolonging the service life

Inactive Publication Date: 2016-04-20
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unreasonable preparations not only cause the death of some bacteria or the inactivation of spores, but also greatly reduce its effect, thus affecting the persistence of effect

Method used

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  • A kind of suspending agent compounded by Bacillus thuringiensis and I. fumigatus and its preparation method
  • A kind of suspending agent compounded by Bacillus thuringiensis and I. fumigatus and its preparation method
  • A kind of suspending agent compounded by Bacillus thuringiensis and I. fumigatus and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Weigh 10g of Bacillus thuringiensis, 10.0g of rose smoke-colored rod pollen 1 billion per gram, 5.0g of peanut oil, 3.0g of castor oil polyoxyethylene ether, 2.0g of fatty alcohol polyoxyethylene ether, and alkylphenol formaldehyde Resin polyoxyethylene ether sodium sulfate 1.0g, epoxy linseed oil 2.0g, sodium methylene bisturphine sulfonate and ethylene glycol 4.0g, white mineral oil to make up to 100g, 4000 rpm, shear for 15 minutes, then Transfer to the grinder, cool with water during the grinding process, the temperature should not exceed 40°C, and grind for 30 minutes at 3000 rpm to prepare a compound oil suspension concentrate of Bacillus thuringiensis and Isora fumigatus with a concentration of 20% by mass .

[0023] 2. Indoor activity determination experiment of 20% I. fumigatus oil suspension concentrate

[0024] Test object: Pieris rapae, a sensitive strain raised indoors;

[0025] Reagent for testing: 20% Bacillus thuringiensis and I. thuringiensis compo...

Embodiment 2

[0032] 1. Weigh 20.0g of Bacillus thuringiensis, 20.0g of 1 billion / g rose smoke-colored rod pollen, 15.0g of peanut oil, 2.0g of polyoxyethylene sorbitan fatty acid ketone, and 1.0g of alkylphenol polyoxyethylene ether , fatty alcohol polyoxyethylene ether 1.0g, alkylphenol polyoxyethylene ether formaldehyde condensate sodium sulfate 1.0g, epoxidized soybean oil 3.0g, glycerin 4.0g, and make up the balance to 100g with methyl oleate, 5000 rpm, cut for 15 minutes, and then transferred to the grinder, cooled with water during the grinding process, the temperature should not exceed 40°C, and ground for 30 minutes at 3000 rpm to obtain Bacillus thuringiensis and Rosa thuringiensis with a mass percentage concentration of 40%. Tobacillus fumigatus complex oil suspension concentrate.

[0033] 2. The experimental method for indoor activity determination is the same as in Example 1.

[0034] The described Bacillus thuringiensis and I. thuringiensis oil suspending agent are diluted 15...

Embodiment 3

[0038] 1. Weigh 20.0g of Bacillus thuringiensis, 1.0g of 1 billion / gram rose smoke-colored rod pollen, 10.0g of peanut oil, 1.0g of alkylarylphenol polyoxyethylene ether phosphate, 1.5g of castor oil polyoxyethylene ether, Sodium alkylbenzenesulfonate 1.0g, epoxy linseed oil 2.0g, sodium lignosulfonate 1.0g, ethylene glycol 3.0g, make up the rest to 100g with methyl oleate, 5000 rpm, cut for 15 minutes , and then transferred to the grinder, cooled with water during the grinding process, the temperature should not exceed 40 ° C, and 3000 rpm for 30 minutes to grind to obtain a compound oil of Bacillus thuringiensis and Isora fumosa with a concentration of 21% by mass suspending agent.

[0039] 2. The experimental method for indoor activity determination is the same as in Example 1.

[0040] The described Bacillus thuringiensis and I. thuringiensis oil suspending agent are diluted 1200 times to spray aphids, and the indoor control effect is 90% in 96 hours after the medicine. S...

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Abstract

The invention relates to a bacillus thuringiensis and isaria fumosorosea compounded suspending agent. The suspending agent is prepared from the following raw materials in percentage by mass: 1-20% of bacillus thuringiensis, 1-20% of isaria fumosorosea, 1-20% of vegetable oil, 3-8% of emulsifying agent, 0.1-5% of dispersing agent, 1-5% of stabilizer, 1-5% of antifreezer and the balance of mineral oil or methyl oleate. The preparation method of the suspending agent comprises the following steps: (1) proportionally mixing bacillus thuringiensis, isaria fumosorosea, vegetable oil, the emulsifying agent, the dispersing agent, the stabilizer, the antifreezer and inert mineral oil or methyl oleate, so as to prepare a mixture; (2) adding the mixture into a shearer, and shearing for 15 minutes at the revolution rate of 3,000-10,000rpm; (3) transferring the sheared mixture into a sand mill for milling. The bacillus thuringiensis and isaria fumosorosea compounded suspending agent has the advantages of high activity, stable effect, long duration, little environmental impact and convenience in use.

Description

technical field [0001] The invention belongs to the technical field of pollution-free pesticide preparations, and in particular relates to a suspending agent compounded by Bacillus thuringiensis and I. fumigatus and a preparation method thereof. Background technique [0002] Bacillus thuringiensis English name bacillusthuringiensis, referred to as Bt, is a class of crystal-producing bacillus including many variants, is currently the largest yield, the most widely used biological insecticide. The main active ingredient of Bacillus thuringiensis is one or several insecticidal crystal proteins (ICPs), also known as δ-endotoxin, which is effective against Lepidoptera, Coleoptera, Diptera, Hymenoptera, Homoptera, etc. Insects, animal and plant nematodes, acarids and other arthropods have specific poisonous activity, but are safe for non-target organisms. Therefore, Bt pesticides have the advantages of specificity, high efficiency and safety to humans and animals. However, comme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01N25/04A01P7/04
Inventor 章玉苹朱天圣王龙江黄华罗振亚
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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