Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of cottonseed protein

A cottonseed protein and cottonseed meal technology, applied in the field of cottonseed processing, can solve the problems of less than 35% protein extraction rate, high production cost, difficult enzymatic hydrolysis process control, etc.

Inactive Publication Date: 2013-12-25
SHIHEZI TIANCHENG GREASE CO LTD
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although alkali-soluble acid precipitation method is an effective method for preparing cottonseed protein, a large amount of waste acid and waste alkaline water are produced during the preparation process; although the protein prepared by salt extraction method has high purity, the protein extraction rate is less than 35%; enzymatic method The quality of the extracted product is good, but the control of the enzymatic hydrolysis process is difficult and the production cost is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of cottonseed protein
  • Preparation method of cottonseed protein
  • Preparation method of cottonseed protein

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] The preparation method of cottonseed protein is as follows:

[0031] (1) Crushing the raw material of cottonseed meal;

[0032] (2) Extract the crushed cottonseed meal with a mixed solvent of ethanol and n-hexane to remove gossypol from the cottonseed meal;

[0033] (3) After the dephenolized cottonseed meal is separated from the mixed solvent, the dephenolized cottonseed meal is obtained;

[0034] (4) Add water to the dephenolized cottonseed meal, then add sodium hydroxide and sodium sulfite to extract cottonseed protein;

[0035] (5) After the dephenolized cottonseed meal is leached, the extract solution is separated, acid is added to adjust the pH of the extract solution to 4-6, acid precipitation, and the precipitation is cottonseed protein.

[0036] The method of the present invention has no special restrictions on the cottonseed meal, which can be the residue after the oil is extracted from the cottonseed by pressing, or the residue after the oil is extracted by...

Embodiment 1

[0072] (1) Pulverize the cottonseed meal;

[0073] (2) The pulverized cottonseed meal is leached with a mixed solvent of ethanol and n-hexane to remove gossypol in the cottonseed meal. The liquid ratio is 1:4 (w / v, g / ml), the extraction temperature is 50°C, and the extraction time is 1 hour, the removal rate of gossypol is 89.60%, and the free gossypol (FG) in dephenolized cottonseed meal is ≤400mg / kg;

[0074] (3) After the dephenolized cottonseed meal is separated from the mixed solvent, the dephenolized cottonseed meal is obtained;

[0075] (4) Add water to the dephenolized cottonseed meal, and then add sodium hydroxide and sodium sulfite to extract cottonseed protein. The ratio of solid to liquid between the dephenolized cottonseed meal and the infusion solution is 1:15, the temperature is 60°C, and the extraction time is 1 hour. The mass ratio of sodium hydroxide and sodium sulfite in the soaking liquid is 1:1, and the total concentration of sodium hydroxide and sodium...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of cottonseed protein, which comprises the steps that (1) cottonseed meal is dephenolized; and (2) the cottonseed protein is extracted from the dephenolized cottonseed meal, wherein dephenolization of the cottonseed meal is to leach the cottonseed meal with a mixed solvent of alcohol and n-hexane, and a volume ratio of alcohol to n-hexane is (1-5):5. The dephenolized cottonseed meal is leached by a mixed aqueous solution of sodium hydroxide and sodium sulfite, and the cottonseed protein is obtained by acid precipitation of a leaching solution. With the adoption of the method, a removed rate of gossypol can reach above 80%; free gossypol (FG) is less than or equal to 400mg / kg; a yield of the cottonseed protein reaches above 60%; and the purity is greater than 90%.

Description

technical field [0001] The invention belongs to the field of cottonseed processing, and in particular relates to a method for preparing cottonseed protein. Background technique [0002] my country is the world's largest cotton-producing country, with an annual cottonseed output of 900×10 4 More than t, its main producing areas are in the North China Plain and the Northwest Plain. Cottonseed is not only an important vegetable oil, but also contains about 20% protein. It is an important oil and protein crop next to soybean in the world today. Therefore, for countries all over the world, cottonseed meal, a by-product of cottonseed oil extraction, has become an important Plant protein source. Due to the existence of gossypol in cottonseed meal, the existing cottonseed meal processing method generally includes two steps: dephenolization (also known as detoxification) of cottonseed meal, and then extracting cottonseed protein from the dephenolized cottonseed meal. At present, t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23J1/14
Inventor 王春荣罗鹏陈静陈国刚
Owner SHIHEZI TIANCHENG GREASE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com