Diabetes-inducing high-fat feed and application thereof to preparation of diabetic foot ulcer rat experimental model

A technology for high-fat feed and diabetes, applied in applications, animal feed, animal feed, etc., can solve problems such as large performance gaps and large model differences

Active Publication Date: 2013-12-25
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Claims
  • Application Information

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Problems solved by technology

The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate the arterial disease, neuropathy or infection of diabetic foot. Due to the different research purposes, the methods of establishing the models are various, and the differences between the models are quite large. There is a large gap with the actual clinical performance

Method used

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  • Diabetes-inducing high-fat feed and application thereof to preparation of diabetic foot ulcer rat experimental model

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Effect test

Embodiment 1

[0016] Rat type 2 diabetes was induced by high-fat diet combined with streptozotocin, and the rat model of diabetic foot ulcer was made by using a scalding instrument to make deep second-degree scald-induced skin ulcers.

[0017] 1 Materials and methods

[0018] 1.1 Animals: SD male rats, SPF grade, body weight (180±10) g, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0019] 1.2 Main reagents and drugs: Streptozotocin (STZ), purchased from Alexis; high-fat feed (ingredients: basal feed 73%, lard 20%, milk powder 2%, cholesterol 1%, sucrose 4%; Among them, the basic feed formula: barley 15%, wheat 31%, corn 15%, bran 15%, fish meal 6%, soybean meal, oil and salt 18%, the basic feed was purchased from the Chinese People's Liberation Army Naval Medical Research Institute), high The preparation method of the fat feed is as follows: first, mix 15% barley, 31% wheat, 15% corn, 15% bran, and 6% fish meal in proportion, and mix t...

Embodiment 2

[0029] Example 2 Western medicine-external application of recombinant bovine basic fibroblast growth factor (trade name: Beifuji) intervention test results

[0030] Methods: The diabetic control group (DM) was untreated; the normal ulcer group (N), the diabetic ulcer control group (DU), the normal saline dressing was changed every day, the diabetic ulcer treatment group (DU-t), the wound of Beifuji 800iu was changed every day medicine. The wound area after healing was measured at 0d, 3d, and 10d after dressing change. After healing, 3% sodium pentobarbital was 2ml·Kg. -1 The dose was intraperitoneal injection anesthesia, blood was collected from inferior vena cava, serum was separated by centrifugation, and skin tissue was collected for examination.

[0031] Data processing: SPSS16.0 was used for statistical analysis, and measurement data were Indicates that the data conforming to normality and homogeneity of variance were analyzed by one-way ANOVA, the components were test...

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Abstract

The invention discloses a diabetes-inducing high-fat feed. The high-fat feed is prepared from the following components according to weight percentage: 73 percent of basal feed, 20 percent of lard oil, 2 percent of milk powder, 1 percent of cholesterol and 4 percent of saccharose, wherein the basal feed is prepared from the following components according to weight percentage: 15 percent of barley, 31 percent of wheat, 15 percent of corns, 15 percent of bran, 6 percent of fish meal and 18 percent of bean pulp, oil and salt. In addition, the invention also discloses application of the high-fat feed to preparation of a diabetic foot ulcer rat experimental model. The high-fat feed is used for preparing the diabetic foot ulcer rat experimental model. The diabetic ulcer animal experimental model detects the change of biochemical criteria after drug intervention, and a result shows that the model has the characteristic of diabetic foot ulcer, the ulcer wound healing time is long, the model is sensitive to drugs, and the like.

Description

technical field [0001] The invention belongs to the field of experimental animal models, in particular to a high-fat feed for inducing diabetes and its application in preparing an experimental model of diabetic foot ulcer rats. Background technique [0002] With the sharp increase in the incidence of diabetes in my country, the number of patients with diabetic ulcers has also increased. The establishment of a disease model that truly simulates diabetic ulcers at the overall animal level is a medical study to explore the pathogenesis of diabetic ulcers and preclinical pharmacodynamic evaluation. It is of great scientific and clinical significance. [0003] At present, the animal models of diabetic foot ulcers are mostly induced animal models. The results of literature studies show that the existing animal models of diabetic foot ulcers mostly simulate the arterial disease, neuropathy or infection of diabetic foot disease. Due to the different research purposes, the model esta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/10A23K1/16A01K67/02
Inventor 柳国斌韩强
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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