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Lactic acid bacterium used for fermenting cereal and applications thereof

A technology of lactic acid bacteria and Lactobacillus plantarum, applied in the directions of fermentation, microorganism-based methods, bacteria, etc., can solve the problem that the efficacy needs to be further improved, and achieve the effect of low production cost and easy quality control.

Active Publication Date: 2013-12-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current reports have confirmed that lactic acid bacteria have the function of preventing and treating tumors, but their efficacy needs to be further improved

Method used

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  • Lactic acid bacterium used for fermenting cereal and applications thereof
  • Lactic acid bacterium used for fermenting cereal and applications thereof
  • Lactic acid bacterium used for fermenting cereal and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Weigh 20.0g of crushed fresh wheat germ powder into a 500mL conical flask, add 100ml of distilled water to it, then add 0.4g of Lactobacillus plantarum Dy-1, and mix well; seal the conical flask with gauze , placed in a constant temperature incubator and fermented at 30°C for 24h, centrifuged at 12000rpm / min for 20min to collect the supernatant and freeze-dry to obtain fermented wheat germ extract (LFWGE)

[0052] Weigh 0.5g of LFWGE and dissolve it in 50ml of deionized water, pass through a 0.22um filter membrane, and filter the filtrate according to 0.125mg / mL, 0.25mg / mL, 0.5mg / mL, 1.0mg / mL, 2.0mg / ml, 4.0 mg / ml were added to HT-29 colon cancer cells, and the cells were cultured for 24h, 48h, and 72h, and the inhibition rate of LFWGE on colon cancer HT-29 cells was determined by MTT method ( figure 2 ). The results showed that the concentration of the extract was positively correlated with the inhibition rate of tumor cell proliferation, and the maximum inhibition ra...

Embodiment 2

[0055] The above-prepared LFWGE was dissolved in physiological saline to prepare aqueous solutions with a concentration of 0.2 g / ml and 0.1 g / ml, and treated through a sterile membrane for use. Human HT-29 colon cancer cells were inoculated subcutaneously into the abdomen of 4-6 week-old BALB / c nude mice, and 2 days later, 2 g / kg / d, 1 g / kg / d, 2 g / kg / d+5-FU (25 mg / kg / d, i.p, 7 days) dose of LFWGE was administered to tumor-bearing nude mice for 30 consecutive days. ) compared with the 5-FU group, and the antitumor effect of LFWGE was determined by measuring the change of tumor volume and the final tumor inhibition rate ( Figure 5 ,6). The results showed that the tumor inhibition rates of 2g / kg / d, 1g / kg / d, 2g / kg / d+5-FU LFWGE dose groups reached 49.37%, 40.7%, 47.8%, respectively.

Embodiment 3

[0057] Weigh 20.0g of barley flour crushed through a 60-mesh sieve into a 500mL conical flask, add 1:6 water to it and mix evenly, first use 0.015% β-glucanase to hydrolyze at 40°C for 30min, then use 4 % Lactobacillus plantarum (Lactobacillus plantarum Dy-1) was fermented at 30°C for 48h. After the fermentation was completed, the FBE was centrifuged at room temperature at 6000rpm / min for 20min, and the supernatant was collected and freeze-dried for 24h to obtain the fermented Barley Extract (FBE).

[0058] Weigh 0.5g of FBE and dissolve it in 50ml of deionized water, pass through a 0.22um filter membrane, and filter the filtrate according to 0.125mg / mL, 0.25mg / mL, 0.5mg / mL, 1.0mg / mL, 2.0mg / ml, 4.0 mg / ml were added to HT-29 tumor cells, and the cells were cultured for 24h, 48h, and 72h, and the inhibitory rate of FBE to colon cancer HT-29 cells was determined by MTT method. The results showed that the concentration of the extract was positively correlated with the inhibition ...

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Abstract

The invention discloses a lactic acid bacterium used for fermenting cereal and applications thereof, and belongs to the field of food biotechnology. Lactic acid bacterium Dy-1, suggested classification name is Lactobacillus plantarum, is delivered to China General Microbiological Culture Collection Center, Institute of Microbiology, China Academy of Science, in No.1 yard, No.3 building, Beichenxi road, Chaoyang district, Beijing, China, at April, 18th, 2012 for preservation, and the preservation number is CGMCC No.6016. Applications of the lactic acid bacterium comprise following steps: wheat germ or barley is fermented by freeze-dried powder of the lactic acid bacterium; a fermentation broth is subjected to centrifugation so as to obtain a supernatant; and the supernatant is subjected to freeze drying so as to obtain a fermentation extract. The fermentation extract possesses significant apoptosis promoting and propagation inhibiting effects on HT-29 colon cancer cells and SGC-7901 stomach cancer cells, and significant inhibiting effect on tumor growth of HT-29 colon cancer induced tumor-bearing nude mice models, and is capable of improving immunity of the HT-29 colon cancer induced tumor-bearing nude mice models.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to lactic acid bacteria used for fermenting wheat germ, barley and other grains, and realizes biotransformation of nutrients in grains through lactic acid bacteria fermentation to prepare fermented extracts capable of inhibiting tumor cell proliferation and tumor growth. Background technique [0002] At present, the number of deaths caused by malignant tumors in China accounts for 1 / 4 of the world's, and the number is still rising. The treatment of cancer mainly includes surgery, chemotherapy, radiotherapy and targeted therapy (immunotherapy, gene therapy, angiogenesis inhibitors and targeted therapy), among which drug therapy is the main form at present. However, the anti-tumor drugs currently widely used have high toxicity and side effects. The development of natural, safe, non-toxic and side-effect anti-tumor active substances and their functional products is the desire of cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/25
Inventor 董英程新崔恒林肖香伍静
Owner JIANGSU UNIV
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