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A kind of traditional Chinese medicine composition for treating cancer and preparation method thereof

A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition for treating cancer and preparation field thereof, can solve the problems of bone marrow suppression, toxic and side effects, tumor recurrence, etc., and achieve the effects of symptom improvement and significant curative effect

Inactive Publication Date: 2015-09-23
天津天中医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high degree of malignancy of the tumor, the current treatment methods are difficult to achieve good curative effect, leading to tumor recurrence and metastasis, and there are some shortcomings of the treatment methods, such as the side effects of chemotherapy often lead to bone marrow suppression

Method used

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  • A kind of traditional Chinese medicine composition for treating cancer and preparation method thereof
  • A kind of traditional Chinese medicine composition for treating cancer and preparation method thereof
  • A kind of traditional Chinese medicine composition for treating cancer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 Chinese medicine composition and its preparation

[0049] [1] Ingredients and preparation of traditional Chinese medicine composition

[0050] The traditional Chinese medicine composition is composed of the following raw materials in proportion by weight: 15 parts of sarcophagus, 20 parts of raw land, 15 parts of jiao corrugated, 30 parts of sea clam shells, 10 parts of ocher seeds, 10 parts of clematis, 12 parts of pulsatilla, 12 parts of figs, 20 parts of burnt malt, 10 parts of nepeta, 30 parts of raw barley, 60 parts of willow root, 15 parts of sunburnt gold, 12 parts of Prunella vulgaris, 40 parts of horsetail, 20 parts of cornus, 10 parts of parsnip copies, Wang Buliuxing 15 copies.

[0051] In this embodiment, the Chinese medicine composition is composed of 15 grams of Sargassum, 20 grams of Rehmannia, 15 grams of cockles, 30 grams of sea clam shells, 10 grams of ocher seeds, 10 grams of Clematis, 12 grams of Pulsatilla, 12 grams of Fig. gram, 20 g...

Embodiment 2

[0071] Embodiment 2: pharmacodynamic research

[0072] [1] Experimental materials and equipment

[0073] 100% ethanol (Tianjin Chemical Reagent Wholesale Company), fetal bovine serum (Invitrogen, USA), RPMI1640 (Invitrogen, USA), trypsin (Sangon Bioengineering (Shanghai) Co., Ltd.), trypan blue (Beijing Dingguo Changsheng Biotechnology Co., Ltd.), MTT (full name 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenyltetrazoliumromide, 3-(4,5-dimethylthiazolium-2)- 2,5-diphenyltetrazolium bromide salt, trade name thiazolium blue, Thermo, USA), DMSO (dimethyl sulfoxide, Beijing Dingguo Changsheng Biotechnology Co., Ltd.), propidium iodide (Sigma, USA) ; Hemocytometer (Shanghai Qiujing Biochemical Reagent Co., Ltd.), low-speed desktop centrifuge (Changsha Xiangyi Centrifuge Instrument Co., Ltd.), enzyme-linked immunoassay (BIO-RAD, USA), flow cytometer (BD FACSDiVa, USA), decolorization shaker (Beijing Detianyou Technology Development Co., Ltd.), CO2 incubator (sanyo, Japan), inverted m...

Embodiment 3

[0120] Example 3 Analysis of the role of traditional Chinese medicine composition on the gene expression profile of lung cancer cells

[0121] [1] The total RNA sample preparation method is as follows (adherent cell lung cancer A549 cell line):

[0122] 1. Culture the cultured lung cancer A549 cells to the logarithmic growth phase, add 10 μl of 100 mg / ml traditional Chinese medicine composition to the experimental group, and add 10 μl of PBS to the control group; put them into the incubator to continue culturing, and the drug will act for 48 hours;

[0123] 2. Discard the culture medium, wash with PBS for 2-3 times; add 1.5ml TRLzol reagent to the culture flask respectively, beat repeatedly with a 1ml pipette tip, so that TRLzol contacts the surface of the culture flask with all cells for full digestion; transfer to RNase-free Repeatedly beat the cells with a disposable syringe in the test tube until no clumps of cells can be seen, and the whole solution should be cool and not...

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Abstract

The invention relates to a traditional Chinese medicine composition used for treating cancer. The traditional Chinese medicine composition comprises following raw materials, by weight, 15 portions of herba sarcandrae, 20 portions of radix rehmanniae, 15 portions of parched concha arcae, 30 portions of concha meretricis seu cyclinae, 10 portions of haematitum, 10 portions of radix clematidis, 12 portions of the root of Chinese pulsatilla, 12 portions of ficus carica, 20 portions of dark malt, 10 portions of herba schizonepetae, 30 portions of raw coix seed, 60 portions of willow root, 15 portions of roasted endothelium corneum gigeriae galli, 12 portions of spica prunellae, 40 portions of scouring rush, 20 portions of common macrocarpium fruit pulp, 10 portions of radix saposhnikoviae and 15 portions of semen vaccariae. It is shown by the results of in vivo and in vitro experiments that the traditional Chinese medicine composition possesses obvious inhibition effects on growth of human lung cancer A549 cell line and human lung cancer NCI-H460 cells. The traditional Chinese medicine composition can be used for treating cancer, and especially for treating of lung cancer.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for treating cancer and a preparation method thereof. Background technique [0002] Malignant tumor, also known as cancer, refers to a serious disease in which cells lose control, proliferate abnormally, continue to grow and then invade adjacent normal tissues, and can be transferred to other parts of the body through blood vessels, lymphatic vessels and body cavities, eventually threatening human life. In my country, malignant tumors rank second among various causes of death, and rank first in some cities; and in the past 20 years, the morbidity and mortality of tumors have shown an obvious upward trend, and the incidence of lung cancer ranks first. At present, the clinical treatment of tumors mainly uses methods such as surgical resection, radiotherapy, chemotherapy, biological therapy and traditional Chinese medicine treatm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8998A61P35/00A61K35/57A61K35/618
Inventor 刘鸿生张晓东叶丽虹
Owner 天津天中医药科技有限公司