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Cordyceps liquid culture medium and application thereof

A liquid culture medium, Cordyceps sinensis technology, applied in the application, fertilizer mixture, fertilization device and other directions, can solve the problems of easy infection of miscellaneous bacteria, long cultivation period, unreasonable nutrient composition structure, etc. , the effect of high conversion efficiency

Inactive Publication Date: 2015-06-24
东方中科生命科学有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, scientific research workers have published some related patents and papers, disclosing some Cordyceps sinensis liquid culture medium and culture methods, but most of them belong to the commonly used liquid culture medium, and the nutrient composition structure in the substrate is unreasonable, so it will produce in liquid culture. The conversion efficiency is not high, the culture cycle is long, and it is easy to be infected with bacteria, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1. Shake flask cultivation in Cordyceps sinensis fermentation factory

[0013] The medium components include 5.0g corn flour, 15.0g soybean flour, 1.0g peptone, 5.0g fish meal, 2.0g silkworm chrysalis powder, 0.1g magnesium sulfate, 0.02g potassium dihydrogen phosphate, 0.02g dipotassium hydrogen phosphate, 0.15g multivitamin powder.

[0014] The multivitamin powder is prepared by mixing 1.0-2.0 vitamin B1, 1.0-2.0 vitamin B2 and 0.05-0.15 riboflavin by weight.

[0015] In the Cordyceps fermentation factory, use 1000ml triangular flasks to carry out liquid culture of Cordyceps sinensis strains. The liquid volume in each triangular flask is 200ml. Add the above materials into 1000ml triangular flasks, dilute to 200ml with water, stir and mix well, and heat to 100 degrees Celsius , until all are uniformly dissolved in the solution.

[0016] Insert 1 piece of parent bacterial block with a diameter of 1cm into the triangular flask, and cultivate it for 8 days ...

Embodiment 2

[0017] Example 2. Seed tank cultivation and fermenter cultivation in the Cordyceps sinensis fermentation factory

[0018] In the Cordyceps sinensis fermentation factory, the first-level seed tank of 50L, the second-level seed tank of 500L, and the fermentation tank of 5000L are used to gradually enlarge and cultivate Cordyceps sinensis. Flour and soybean flour are replaced with the same amount of coarse grain compound raw materials (which contain millet bran, sorghum husk, buckwheat coarse flour, mixed in the same proportion), peptone and fish meal are replaced with the same amount of coarse protein powder (including corn bran, Coarse peptone, coarse soybean powder, mixed in the same proportion), under the premise of ensuring the nutritional structure of the substrate, the production cost is reduced, and the remaining ingredients and proportions are the same as in Example 1, except that the actual input amount of each ingredient is in equal proportions according to the ca...

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PUM

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Abstract

The invention discloses a Cordyceps sinensis liquid culture medium and an application thereof, which solves the problems of slow growth, long period and easy pollution of Cordyceps sinensis during liquid culture. The liquid culture medium provided by the invention is composed of corn flour, soybean flour, peptone, fish meal, silkworm chrysalis powder, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, compound vitamins and water. The culture medium for liquid culture of Cordyceps sinensis of the present invention enriches the nutrient structure of the substrate, can promote the growth of mycelium of liquid culture of Cordyceps sinensis, shorten the culture time, and reduce the chance of pollution. In actual production, it can provide high-quality liquid medium for the steps of shake flask culture, seed tank culture or fermenter culture in the Cordyceps sinensis fermentation factory.

Description

technical field [0001] The invention belongs to a liquid culture medium of Cordyceps sinensis and application thereof. Background technique [0002] Cordyceps sinensis is a medicinal fungus (the Latin scientific name is Cordyceps sinensis, also written as Ophiocordyceps sinensis), which gradually develops from an ascomycete invading the larvae of bat moths. The active ingredients contained in Cordyceps sinensis, such as cordycepic acid, cordycepin, and cordyceps polysaccharide, have many medicinal effects, and are effective in treating diseases such as anemia, weakness, impotence, spermatorrhea, chronic deficiency after illness, and persistent cough, especially for the kidneys. Health effects. Since wild Cordyceps sinensis is becoming rarer and rarer, the production of Cordyceps mycelium by liquid fermentation method is becoming more and more important. [0003] Cordyceps sinensis mycelium fermentation mostly adopts liquid fermentation method, which is shorter than solid f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G1/00
Inventor 李文新陈春森廖金永
Owner 东方中科生命科学有限责任公司