Bacillus subtilis X3 and application thereof for reducing generation of hydrogen sulfide in buffalo dung

A Bacillus subtilis, hydrogen sulfide technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., to achieve the effect of reducing hydrogen sulfide gas generation, high-efficiency hydrogen sulfide gas generation, and broad application prospects

Inactive Publication Date: 2014-01-01
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And at present really use microorganism to directly remove in cow dung, especially

Method used

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  • Bacillus subtilis X3 and application thereof for reducing generation of hydrogen sulfide in buffalo dung
  • Bacillus subtilis X3 and application thereof for reducing generation of hydrogen sulfide in buffalo dung
  • Bacillus subtilis X3 and application thereof for reducing generation of hydrogen sulfide in buffalo dung

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Isolation of Bacillus subtilis X3 (Bacillus subtilis X3, No. CCTCC NO: M2013261).

[0046] The isolation of Bacillus subtilis X3 (Bacillus subtilis X3 number is CCTCC NO: M2013261) includes two parts: sample screening and strain isolation:

[0047] 1. Preliminary screening of sample sources containing highly efficient deodorizing microorganisms:

[0048] (1) Weigh 10g of the sewer sludge outside the cattle farm, fish pond water, sewer sludge inside the cattle farm, mud beside the fish pond, and dried cow manure on the grassland, and put them into the centrifuge tube (note: a small space should be left to not fill it up) , to avoid volume increase and expansion after freezing), store at -20°C, and store 3 tubes for each sample.

[0049] (2) Weigh 20g of fresh cow dung and add it to the petri dish.

[0050] (3) Weigh the sewer sludge outside the cattle farm, fish pond water, sewer sludge inside the cattle farm, mud beside the fish pond, dry cow dung on the grassland, an...

Embodiment 2

[0074] Deodorizing microorganism Bacillus subtilis X3 (Bacillus subtilis X3) to reduce hydrogen sulfide gas production in buffalo manure:

[0075] (1) Add 50g of cow dung into a 1000ml large plastic beaker, inoculate the microbial culture solution with deodorizing effect in the preliminary screening test according to 20% inoculation amount, and use the same amount of sterile water as a control;

[0076] (2) Put a small beaker containing 20ml zinc ammonium complex salt solution in a large beaker to absorb hydrogen sulfide (see Appendix 1 for the determination method). The large beaker was sealed with two layers of plastic film and one layer of plastic wrap, and two replicates were done, and incubated at room temperature.

[0077] (3) Take a small beaker to detect the release of ammonia and hydrogen sulfide every 2 days for 8 consecutive days.

[0078] The result is as follows:

[0079] The results in Table 4 show that the strain Bacillus subtilis X3 (Bacillus subtilis X3) can...

Embodiment 3

[0083] Identification of deodorizing microorganism Bacillus subtilis X3:

[0084] Inoculate the microorganism Bacillus subtilis X3 (Bacillus subtilis X3) with greatly reduced hydrogen sulfide release in the liquid medium, cultivate it in the incubator for 16 hours, and then take samples for observation under the microscope; in addition, take 2ml samples and add 2ml cryopreservation tube, stored at -80°C, used for DNA extraction, and then used primer 8f(5'-CACGGATCCAGAGTTTGAT(C / T)(A / C)TGGCTCAG-3') / 1510r(5'-GTGAAGCTTACGG(C / T ) TACCTTGTTACGACTT-3') amplifies the bacterial 16S rRNA sequence and sends it for detection.

[0085] Microscopic identification and sequencing results are as follows:

[0086] The morphology of the isolated and cultured microbial colonies was observed under a microscope. Colony morphology: milky white, round, with smooth edges, moist, thicker in the middle of the colony, and gradually thinner towards the surroundings. The bacterial DNA was extracted and ...

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Abstract

The invention discloses Bacillus subtilis X3 and application thereof for reducing generation of hydrogen sulfide in buffalo dung. The Bacillus subtilis X3 is preserved in China Center for Type Culture Collection on June 16, 2013, the serial number is CCTCC NO: M2013261, and the preservation address is Wuhan University, Wuhan, China. Soil and the like in a buffalo farm are used as strain separation sources, the Bacillus subtilis X3 is cultured at 37 DEG C for 24h and is directly inoculated into a sealed beaker filled with buffalo dung in a proportion of 20% (W/W), and the Bacillus subtilis X3 is cultured for 2-8 days at room temperature to reduce hydrogen sulfide. Compared with a control group added with an equal proportion of sterile water, the Bacillus subtilis X3 can reduce the generation of hydrogen sulfide in the buffalo dung by 19.59-52.74% and has the advantages of high efficiency, low nutrition requirements, simple operation and high safety.

Description

technical field [0001] The invention relates to a new bacterial strain Bacillus subtilis X3 (Bacillus subtilis X3) capable of reducing the generation of hydrogen sulfide gas in buffalo manure and its application in reducing the generation of hydrogen sulfide in manure. Background technique [0002] In recent years, my country's livestock and poultry breeding industry has developed rapidly, and the proportion of large-scale breeding has continued to increase. While meeting the increasing demand for livestock and poultry products and improving people's living standards, it has also brought a series of ecological problems. During the production, treatment, storage and discharge of livestock manure, a large amount of malodorous substances will be produced. These malodorous substances have complex components, mainly containing chemical substances such as ammonia, sulfide, amines and volatile fatty acids. Stench refers to all substances that stimulate the olfactory organs to cause...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F11/02C12R1/125
Inventor 杨承剑李婉梁辛梁贤威韦升菊李舒露杨炳壮邹彩霞李丽莉
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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