Shellfish sn RNA U6 gene, primer, and applications thereof

Abstract

The invention relates to the field of molecular biology, and especially relates to applications of a complete sequence of shellfish sn RNA U6 gene, and a primer thereof on expression analysis of shellfish micro RNA. The invention discloses a reference gene and a primer thereof in mi RNA relative quantification expression analysis based on the fluorescence quantification PCR. We obtain the complete sequence of crassostrea gigas U6 gene by cloning, whose homologous gene human sn RNA U6 has a constant expression and can be used as an expression analysis reference of mi RNA. The obtained expression result by taking the human sn RNA U6 as the reference is highly identical to the sequencing expression profile, and thus it is proved that the U6 gene is suitable for being taken as a reference of mi RNA expression analysis.

Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to the application of the full sequence of a shellfish snRNA U6 gene and its primers to the expression analysis of shellfish microRNA. Background technique

[0002] Shellfish, belonging to the clambranch (or bivalves) in the mollusk phylum, common species include oysters, mussels, clams, and razor clams. There are about 10,000 species in existence, 80% of which live in the ocean. Most species of shellfish are edible. Many shellfish are tender, delicious, nutritious and have high economic value. They are important aquaculture objects in the world.

[0003] MicroRNA (miRNA) is a kind of small molecule non-coding single-stranded RNA that widely exists in the biological world. It is about 21-24nt long. It is widely expressed in various developmental stages and different tissues and organs, and participates in various physiological activities of organisms. Because of its importance in ...

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