Method for extracting lentinus edodes total alkaloid

A technology of total alkaloids and extraction methods, applied in food extraction, food science, medical raw materials derived from fungi, etc., can solve the problems of shortened extraction time, low extraction efficiency, long extraction time, etc., and achieves less extraction residue and extraction. High efficiency and low solvent consumption

Active Publication Date: 2015-03-04
广东君道营养科技有限公司
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Problems solved by technology

[0005] The problem to be solved by the present invention is the long extraction time and low extraction efficiency of the traditional solvent extraction method. The rapid solvent extraction method (ASE) is used to study the extraction process conditions of the total alkaloids of Lentinus edodes, which improves the extraction efficiency, shortens the extraction time, and effectively reduces cost

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  • Method for extracting lentinus edodes total alkaloid

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Embodiment 1

[0024] Dried shiitake mushrooms were crushed and sieved to obtain 60-mesh shiitake mushroom powder. Take 4g of shiitake mushroom powder and 6g of diatomaceous earth and mix them in a mass ratio of 4:6. Grind them evenly to obtain a mixed sample; add filter paper to the bottom of the extraction pool in advance, and move the mixed sample to The extraction cell is fully covered, and the extraction cell is placed on the extraction cell fixture after tightening the top cell cap. With 80% ethanol as the extraction solvent, the parameters of the ASE extraction method were set: extraction temperature 140 °C, extraction pressure 1500 psi, extraction 2 times, extraction 15 min, purge volume 90%, and purge time 120 s. Start the accelerated solvent extractor and stop automatically after the extraction is complete. The extract was filtered, evaporated to dryness by a rotary evaporator, dissolved in 20 mL of hydrochloric acid with a mass fraction of 2%, and filtered by a vacuum pump. The fi...

Embodiment 2

[0026] Dried shiitake mushrooms were crushed and sieved to obtain 60-mesh shiitake mushroom powder. Take 4g of shiitake mushroom powder and 6g of diatomaceous earth and mix them in a mass ratio of 4:6. Grind them evenly to obtain a mixed sample; add filter paper to the bottom of the extraction pool in advance, and move the mixed sample to The extraction cell is fully covered, and the extraction cell is placed on the extraction cell fixture after tightening the top cell cap. With 95% ethanol as the extraction solvent, the parameters of the ASE extraction method were set: extraction temperature 100 °C, extraction pressure 1500 psi, extraction 2 times, extraction 10 min, purge volume 90%, and purge time 120 s. Start the accelerated solvent extractor and stop automatically after the extraction is complete. Filtrate the crude extract, evaporate it to dryness with a rotary evaporator, dissolve it with 20mL hydrochloric acid with a mass fraction of 2%, filter it with a vacuum pump, a...

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Abstract

The invention discloses a method for extracting a lentinus edodes total alkaloid. The method comprises the following steps: mixing and grinding uniformly lentinus edodes powder and kieselguhr so as to obtain a mixture; transferring the mixture to an extraction tank with filtering paper at the bottom and fully filling the extraction tank; carrying out accelerated solvent extraction; setting parameters of the accelerated solvent extraction, wherein an extraction solvent is ethanol with the volume percentage of 60%-95%, an extraction temperature is 60-180 DEG C, the extraction pressure of 1000psi-3000psi, the extraction is carried out 1-5 times, extraction time is 5min-30min, a blowing and washing volume is 10%-100% of the volume of the extraction tank, blowing and washing time is 80s-300s; filtering an obtained extracting solution and evaporating by a rotary evaporator; dissolving by using hydrochloric acid and filtering; adjusting the pH (Potential of Hydrogen) value of a filtering solution by using concentrated liquid ammonia; then transferring the solution into a separating funnel to be extracted by chloroform; taking a chloroform layer; finally, concentrating in vacuum and drying. In the method, a rapid solvent extraction method is adopted to extract the lentinus edodes total alkaloid, and the method disclosed by the invnetion has the advantages of short consumed time, small quantity of consumed solvents, high extraction efficiency, small quantity of extraction residues, simple and automatic operation process, various operation modes and the like.

Description

technical field [0001] The invention belongs to the field of food industry, in particular to a method for extracting total alkaloids of shiitake mushrooms. Background technique [0002] Lentinus edodes is a basidiomycete of Pleurotaceae. It is a kind of edible and medicinal fungus, which has the functions of reducing phlegm and regulating qi, invigorating the spleen and appetizing, treating wind and breaking blood, and protecting the liver. With the development of modern biochemistry and medicine, the development of various health foods, drinks, condiments and medicines using shiitake mushrooms as raw materials has attracted the interest and attention of many life science research institutes or bio-high-tech companies at home and abroad. But for a long time, the polysaccharide active substances in shiitake mushrooms have been studied more, but the research on alkaloids of shiitake mushrooms has not been reported in our country. [0003] Alkaloids are an important class of n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/28A61K36/06A23L31/00
CPCA23L31/00A23V2002/00A23V2300/14A23V2250/208A23V2300/10
Inventor 何晋浙冯婷婷孙培龙陈洪波
Owner 广东君道营养科技有限公司
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