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Casein phosphopeptide and zinc chelate compound

A technology of casein phosphopeptide and phosphopeptide zinc, which is applied in the field of preparing casein phosphopeptide zinc chelate, and achieves good stability

Inactive Publication Date: 2014-01-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technology of preparing casein phosphopeptide zinc by chelating casein phosphopeptide with zinc has not yet been patented and reported in the literature.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of casein phosphopeptide zinc chelate mainly includes the following steps

[0025] Step 1: Take 17.5g of casein and dissolve it in 50ml of 0.25mol / L sodium hydroxide solution at 60℃~70℃. After dissolving completely, dilute the volume to 250g with deionized water and make it to a concentration of 7% Casein solution

[0026] Step 2: Add 0.07g of alkaline protease with a specific activity unit greater than or equal to 200 U / mg to the casein solution, place it in a 55℃~60℃ water bath, adjust the pH value to 10.0~10.5 with sodium hydroxide solution. Incubate the enzymatic hydrolysis at 55℃~60℃ for 3.0h, and after the reaction is finished, treat the enzyme in a boiling water bath at 100℃ for 8min~10min to inactivate the enzyme to obtain the enzymatic hydrolysis solution;

[0027] Step 3: Adjust the pH value of the enzymatic hydrolysate to 4.6 with hydrochloric acid, centrifuge at 3500r / min for 15 minutes, and take the supernatant, which is the casein hydrolys...

Embodiment 2

[0032] Step 5: Adjust the pH to 6.5, the mass ratio of casein phosphopeptide to zinc ion is 3:1, and the chelating temperature of the water bath is 55°C;

[0033] Other operation steps are the same as in Example 1.

Embodiment 3

[0035] Step 5: Adjust the pH to 6.5, the mass ratio of casein phosphopeptide to zinc ion 4:1, and the chelating temperature of the water bath at 65°C;

[0036] Other operation steps are the same as in Example 1.

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Abstract

The invention relates to a casein phosphopeptide and zinc chelate compound. The casein phosphopeptide and zinc chelate compound is white solid powder, is tasteless, is slightly dissolved into water, is easily dissolved into faintly acid water and is not dissolved into organic solvents such as alcohols and the like; the chelation rate of casein phosphopeptide and zinc in the casein phosphopeptide and zinc chelate compound is more than 90%. A method for preparing the casein phosphopeptide and zinc chelate compound comprises the step of subjecting casein phosphopeptide and inorganic zinc to chelation reaction to obtain the casein phosphopeptide and zinc chelate compound, wherein the casein phosphopeptide is obtained through carrying out enzymolysis on casein. According to the invention, a preparation process / technology for casein phosphopeptide and zinc chelation is firstly established, and a novel bioactive peptide zinc nutrition enhancer is obtained. The casein phosphopeptide and zinc chelate compound obtained by the invention can be used as a novel zinc supplement nutrition enhancer to be applied to foods.

Description

Technical field [0001] The invention belongs to the technical field of the preparation process of a functional nutrition fortifier, and specifically relates to a process method for preparing a zinc casein phosphopeptide chelate. Background technique [0002] As one of the essential trace elements for the human body, zinc is a nutrient element with important physiological functions. It plays an important role in the body's metabolism and is closely related to physiological functions such as growth and development, reproduction, digestion, immunity, vision, and aging. The results of the survey on the nutritional status of children show that about 26%-27% of children in my country are zinc-deficient, and adults and the elderly in some areas also have symptoms of zinc deficiency. Zinc deficiency in children affects their intellectual development and growth, while zinc deficiency in adults and the elderly affects normal metabolism, leading to reduced immunity and leading to many dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A23K1/16A23C9/152A23C11/10A21D2/24A23L11/65
CPCA21D2/24A23C9/152A23C11/10A23K20/00A23L33/00
Inventor 王志耕张胥卿梅林薛秀恒
Owner ANHUI AGRICULTURAL UNIVERSITY
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