Autofluorescence bone repairing magnetic sustained-release microspheres

A slow-release microsphere and autofluorescence technology, which is applied in the field of bone filling material manufacturing, can solve the problems of high cytotoxicity of cross-linking agents and affect normal tissue growth, etc., and achieves superior biocompatibility, no toxic side effects, and simple preparation method. Effect

Inactive Publication Date: 2015-03-04
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the traditional cross-linking methods use chemically synthesized cross-linking agents. The cross-linking agents themselves have relatively high cytotoxicity, causing the obtained biomaterials to affect the growth of normal tissues after they are implanted into the receptors.

Method used

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  • Autofluorescence bone repairing magnetic sustained-release microspheres
  • Autofluorescence bone repairing magnetic sustained-release microspheres
  • Autofluorescence bone repairing magnetic sustained-release microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Add 1.35g FeCl 3 ·6H 2O, 40ml ethylene glycol, 3.6g sodium acetate, magnetically stirred evenly, placed in a reaction kettle, after 12 hours of reaction, the obtained product was washed several times with absolute ethanol, several times with deionized water, centrifuged, 40°C vacuum drying;

[0044] (2) Sodium alginate was dissolved in deionized water with a mass percentage of 1.2%, and magnetically stirred for 1 hour;

[0045] (3) Add the prepared nano-magnetic Fe to the above solution 3 o 4 Powder, the mass percentage is 0.008%, electric stirring for 1h;

[0046] (4) Add an ethanol solution of icariin, with a mass percentage of 0.02%, and magnetically stir for 1 hour;

[0047] (5) Using a microcapsule forming device with a voltage of 1.3kv, use a syringe to drop the mixture in step (4) into the gel bath to form primary microspheres;

[0048] (6) Submerge the primary microspheres in a chitosan acetic acid solution with a mass percentage of 0.6% for 8 hours, a...

Embodiment 2

[0051] (1) Add 1.35g FeCl 3 ·6H 2 O, 40ml ethylene glycol, 3.6g sodium acetate, magnetically stirred evenly, placed in a reaction kettle, after 12 hours of reaction, the obtained product was washed several times with absolute ethanol, several times with deionized water, centrifuged, 40°C vacuum drying;

[0052] (2) Sodium alginate was dissolved in deionized water with a mass percentage of 1.4%, and magnetically stirred for 1 hour;

[0053] (3) Add the prepared nano-magnetic Fe to the above solution 3 o 4 Powder, the mass percentage is 0.008%, electric stirring for 1h;

[0054] (4) Add an ethanol solution of icariin, with a mass percentage of 0.03%, and magnetically stir for 1 hour;

[0055] (5) Using a microcapsule forming device with a voltage of 1.3kv, use a syringe to drop the mixture in step (4) into the gel bath to form primary microspheres;

[0056] (6) Submerge the primary microspheres in a chitosan acetic acid solution with a mass percentage of 0.8% for 10 hours,...

Embodiment 3

[0059] (1) Add 1.35g FeCl 3 ·6H 2 O, 40ml ethylene glycol, 3.6g sodium acetate, magnetically stirred evenly, placed in a reaction kettle, after 12 hours of reaction, the obtained product was washed several times with absolute ethanol, several times with deionized water, centrifuged, 40°C vacuum drying;

[0060] (2) Sodium alginate was dissolved in deionized water with a mass percentage of 1.5%, and magnetically stirred for 1 hour;

[0061] (3) Add the prepared nano-magnetic Fe to the above solution 3 o 4 Powder, the mass percentage is 0.01%, electric stirring for 1h;

[0062] (4) Add an ethanol solution of icariin, with a mass percentage of 0.03%, and magnetically stir for 1 hour;

[0063] (5) Using a microcapsule forming device with a voltage of 1.3kv, use a syringe to drop the mixture in step (4) into the gel bath to form primary microspheres;

[0064] (6) Submerge the primary microspheres in a chitosan acetic acid solution with a mass percentage of 0.5% for 12 hours, ...

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Abstract

The invention discloses a self-fluorescent bone repair magnetic slow-release microsphere and a preparation method thereof. The nano-Fe3O4 is synthesized by a solvothermal method, and the nano-Fe3O4 is uniformly dispersed in a sodium alginate solution, and the composite has the functions of promoting proliferation and promoting osteoblasts. Differentiation of traditional Chinese medicine - icariin; the mixture is passed through a microcapsule forming device and dropped into a gel bath to form primary microspheres; then the primary microspheres are immersed in chitosan solution, and chitosan and alginic acid are used to The charge complementary properties of sodium form a polyelectrolyte semipermeable membrane, and the composite microspheres are cross-linked with genipin to prepare autofluorescent magnetic sustained-release microspheres for bone repair. The present invention fully considers the requirement of cytotoxicity as an implantable bone repair material, selects chitosan and sodium alginate with excellent biocompatibility and degradability as raw materials, and can be adjusted according to the needs of bone lesions. Different voltages are used to prepare microspheres with different particle sizes.

Description

technical field [0001] The invention belongs to the field of bone filling material manufacture, and in particular relates to autofluorescent bone repair magnetic slow-release microspheres and a preparation method thereof. Background technique [0002] Trauma, bone tissue inflammation, bone defects caused by congenital defects, and bone tissue diseases such as bone cancer are very common in orthopedic clinics. Relying solely on the self-repair ability of bone is relatively limited. Bone transplantation is often used clinically to treat bone tissue diseases. Therefore, the research and development of bone repair materials is of great significance. In recent years, more and more attention has been paid to the research of microspheres and microcapsules in the field of biomedicine. Microsphere is a kind of spherical or quasi-spherical particle made of suitable polymer material as the carrier. The particle size is generally 1-500 μm, the small one can be several nanometers, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/12A61L27/20A61L27/54A61L27/50B01J13/02
Inventor 陈景帝张惠张玉珏潘盼盼张其清
Owner FUZHOU UNIV
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