Method for preparing citric acid by fermentation

A technology of citric acid and fermentation medium, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of low fermentation cost, low citric acid yield and fermentation conversion rate, high fermentation cost, etc., and achieve improvement The effect of fermentation level, reduction of stirring power and reduction of production cost

Active Publication Date: 2015-04-22
COFCO BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the production of citric acid using the prior art, the yield and fermentation conversion rate of citric acid are low, and the defects of high fermentation costs, to provide a citric acid yield and fermentation conversion rate are higher, Method for preparing citric acid by fermentation with low fermentation cost

Method used

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  • Method for preparing citric acid by fermentation
  • Method for preparing citric acid by fermentation

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0067] Preparation of fermentation medium for fermentation

[0068] 1) Grinding of raw materials: Soak the harvested corn in a hot water tank until the water content of the corn is 15% by weight, and then grind it through a grinder (Jiangsu Muyang Group Co., Ltd., 968-3 type) to obtain a starchy raw material grinding product (50% by weight of the crushed particles have a particle diameter less than 0.1 cm);

[0069] 2) Sizing: mix water at 50°C with the crushed product of the starchy raw material obtained in 1) to make a starch slurry. The weight ratio of the crushed product of the starchy raw material to water is 1:3, and the pH of the starchy slurry is Value is 5.5;

[0070] 3) Enzymolysis: mix the starch slurry obtained in step 2) with 1 / 2 enzyme (adding starch The total weight of the enzyme is mixed with 15U / g corn flour pulverized product), and the first injection liquefaction is carried out at 95°C and the pH is 5.5 for 5 minutes, and then the second injection liquefac...

preparation example 2

[0074] Preparation of fermented strain seed solution

[0075] Dilute part of the corn liquefaction solution in step 3) of Preparation Example 1 with water until the total sugar content is 11% by weight, put the culture solution into the seed tank, heat it to 121°C for disinfection, keep it for 20 minutes and then quickly cool it down to 36°C, then Enter Aspergillus niger bacterial classification (Aspergillus niger T01, Tianjin Institute of Industrial Microbiology, be this Aspergillus niger bacterial classification in the embodiment of the present invention, inoculum size is: every milliliter of nutrient solution is a benchmark, inoculates 1 * 10 5 spores), the temperature is 37°C, the ventilation rate is 0.4V / V·min, the air pressure is 0.05-0.06Mpa, the stirring speed is 750rpm, and the strains are carried out under the condition of maintaining the tank pressure during this stage. Cultivate; observe the growth of Aspergillus niger through sampling microscope inspection, acidit...

Embodiment 1

[0077] This example is used to illustrate the method for preparing citric acid by fermentation provided by the present invention.

[0078] The Aspergillus niger seed liquid cultivated in Preparation Example 2 is added to the fermentation medium of Preparation Example 1 to start fermentation, wherein, in terms of per milliliter of fermentation medium, the inoculum size of Aspergillus niger is 3.0 × 10 4 spores, the fermentation conditions include a temperature of 37°C, a culture pressure of 0.04Mpa, an aeration rate of 0.8 volume: (volume·min), and a stirring speed of 850rpm. During the fermentation process, samples were continuously taken to determine the bacterial content in the culture medium.

[0079] When the content of Aspergillus niger in the fermentation medium reached 1.4% by weight, the continuous addition of Tween-60 solution with a concentration of 0.6g / L fermentation broth began in the fermentation medium. Wherein, in terms of per liter of fermentation medium, the...

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Abstract

The invention discloses a method for preparing citric acid by fermentation. The method comprises the following steps: under conditions that the citric acid can be produced, inoculating Aspergillus niger in a fermentation medium for fermentation, and adding an inhibitor in the fermentation medium when the content of the Aspergillus niger in the fermentation medium achieves 1-1.7wt%, wherein the appropriate addition of the inhibitor can increase the content of the Aspergillus niger, but the increment of the Aspergillus niger does not exceed 0.2wt% per hour. In the fermentation of the citric acid by the technical scheme, the excessively fast growth of fermentative strains is effective inhibited, and fermentative bacterium and nutrient substances are all developed to a trend beneficial to production of the citric acid, so that the fermentation level is enhanced. Meanwhile, the balls of the fermentative strains are small and compact, and the mycelium is short, so that on the premise of guaranteeing the required amount of dissolved oxygen for fermentation, the stirring power is lowered, and consequently, the method is easy to operate and the production cost is lowered.

Description

technical field [0001] The invention relates to a method for fermenting and preparing citric acid. Background technique [0002] Citric acid is an important organic acid that can be produced by microbial metabolism at present. It has a wide range of uses, mainly used in the food industry, followed by medicine, textiles, construction and other industrial sectors; it is used in cleaning, rust removal and daily chemicals for various important equipment. There are also important uses in other aspects. [0003] At present, the fermentation technology adopted by the citric acid fermentation industry is usually an intermittent liquid submerged secondary fermentation technology that feeds once and discharges once. The fermentation process is as follows: enzymatic hydrolysis of starchy raw materials to prepare fermentation medium, and then inoculate Aspergillus niger species into the fermentation medium in the fermenter for fermentation to produce citric acid. After the fermentation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 罗虎熊结青卢宗梅王梅吴晓艳
Owner COFCO BIOTECHNOLOGY CO LTD
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