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Morganella morganii and application thereof to fermentation detoxification of barbadosnut cake

A technology of Morganella morganii and Jatropha curcas cake, which is applied in the field of fermentation, can solve the problems of cake nutrient destruction, high cost, and chemical residues, and achieve the effects of reducing toxic components, easy operation, and low cost

Inactive Publication Date: 2014-01-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, physical and chemical methods are mostly used at home and abroad to remove phorbol esters in Jatropha curcas oil meal, but the chemical detoxification operation is complex and cumbersome, which easily leads to chemical residues, and the detoxification range is small and the cost is high; while enzymatic hydrolysis can only degrade A certain type of toxin is highly targeted, and the passivation effect is inconsistent; the efficiency of physical detoxification is low, and it will cause certain damage to the nutrients in the cake

Method used

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  • Morganella morganii and application thereof to fermentation detoxification of barbadosnut cake
  • Morganella morganii and application thereof to fermentation detoxification of barbadosnut cake
  • Morganella morganii and application thereof to fermentation detoxification of barbadosnut cake

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Embodiment 1

[0035] Embodiment 1 The identification feature of Morganella morganella of the present invention

[0036] (1) Physiological and biochemical characteristics

[0037] After culturing the isolated strain of the present invention on NB and crystal violet plates for 24 hours, a single colony on the NB plate was taken for Gram staining observation, combined with the growth of the strain on the crystal violet plate to determine its Gram-negative, and oxidase was carried out. Experiment and trisaccharide iron experiment, the results are shown in Table 1:

[0038] Table 1 Physiological and biochemical characteristics

[0039]

[0040] It can be seen from the results of physiological and biochemical experiments that the zxy-12-4 isolate of the present invention is a Gram-negative bacteria and belongs to intestinal bacteria (GN-ENT). Corresponding GN identification plate.

[0041] (2) Biolog analysis

[0042] Using Biolog microbial automatic analysis system analysis, the results o...

Embodiment 2

[0050] Embodiment 2 adopts the method for detoxification of Jatropha curcas cake fermentation by using Morganella morganii of the present invention

[0051] 1 Materials and Instruments

[0052] 1.1 Materials

[0053] Strain: Morganella morganii zxy-12-4 (Morganella morganii zxy-12-4) of the present invention, referred to as strain zxy-12-4.

[0054] Jatropha curcas cake: crush the dried jatropha curcas cake through a 40-mesh sieve.

[0055] 1.2 Main instruments

[0056] AIRTECH ultra-clean workbench SW-CJ-2F, high performance liquid chromatography with diode array detector (USA, DIONEX company), vertical pressure steam sterilizer LDZX-50KBS, double-layer constant temperature oscillator IS-RSV1, Ultrasonic cleaning instrument SB-5200DTDN, FW135 plant pulverizer, electric heating constant temperature blast drying oven DHG-9070A, SI-234 electronic balance DENVER INSTRUMENT, Haier refrigerator BCD-215KS, ultraviolet spectrophotometer (UV1700), 10ml volumetric bottle, adjustable...

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Abstract

The invention discloses a Morganella morganii zxy-12-4, which is preserved in China Center for Type Culture Collection and has a preservation number of CCTCC NO:M2013185. The invention also discloses application of the Morganella morganii to fermentation detoxification of barbadosnut cake. The Enterobacter gergoviae zxy-12-4 with the preservation number of CCTCC NO:M2013185 can effectively reduce toxic components in barbadosnut cake and increase practical value of barbadosnut cake.

Description

technical field [0001] The invention relates to a strain of Morganella morganii and its application in the fermentation and detoxification of jatropha curcas cake, which belongs to the field of fermentation. Background technique [0002] Jatropha curcas (Jatropha curcas L.) is recognized worldwide as the most likely tree species with great development potential to replace fossil energy in the future, and the biodiesel produced by processing Jatropha curcas oil is close to or even surpasses the national " No. 0" diesel oil and national biodiesel standards, so the production of biodiesel from Jatropha curcas fruit has become an active direction of research and production of biodiesel. Jatropha curcas seed cake (JCSC) is a by-product of the biodiesel production process, which has high protein content and high nutritional value, but because of its high toxicity, it is mainly used as fertilizer or directly discharged In the natural environment, not only a large amount of high-qu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/015C12R1/01A23L5/20
Inventor 陈放张晓喻唐琳刘莹尹利
Owner SICHUAN UNIV
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