Fermentation production method for natural L-(+)-tartaric acid
A production method, tartaric acid technology, applied in the field of microbial fermentation, to achieve the effect of simple process and less pollution
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Embodiment 1
[0025] Embodiment 1: Utilize 5-ketogluconate to produce the screening of L-(+)-tartrate bacterial classification
[0026] 1 Collection of rhizosphere soil samples
[0027] When sampling, take 4-5 holes of normal plants at each point, dig out the root with soil, cut off the aboveground part, leave the root and the soil sample attached to the root, put it into a sample bag, seal the bag mouth, put it in a 4°C refrigerator, and Prepare for microbial isolation.
[0028] 2 Isolation and cultivation
[0029] (1) culture medium
[0030] Enrichment medium: peptone 20g / L, K 2 HPO 4 1.5g / L, glycerin 10g / L, MgSO 4 1.5g / L, agar 15g / L, pH7.2.
[0031] Separation medium: Same as enrichment medium.
[0032] (2) Separation and culture
[0033] Preparation of soil dilution: Weigh 1.0 g of rhizosphere soil, put 99 ml of sterile water into a 250 ml Erlenmeyer flask filled with glass beads, oscillate on a shaker for 5 minutes to disperse the soil evenly, and become a soil suspension (10 ...
Embodiment 2
[0038] The fermentation culture of embodiment 2L-(+)-tartaric acid
[0039] Pick a ring of TUST bacteria from the activated slope and inoculate it into a 250mL Erlenmeyer flask containing 50mL of seed medium for cultivation, and cultivate it on a shaker at 30°C and 170r / min for 24h to obtain a seed solution. The composition of the seed medium is: peptone 20g / L, K 2 HPO 4 1.5g / L, glycerol 10g / L, MgSO 4 1.5g / L, agar 15g / L, pH7.2, sterilized by high pressure steam at 121℃ for 20min.
[0040] The seed solution was inoculated into the fermentation medium with 10% inoculum amount, fermented in shake flasks at 30°C and 170r / min, and cultured for 5 days. The composition of the fermentation medium is: 5-keto-gluconate potassium 60g / L, MgSO 4 0.25g / L, KH 2 PO 4 0.6g / L, urea 2g / L, cornmeal 10g / L.
[0041] After 5 days of fermentation, the output of L-(+)-tartaric acid was 0.3g / L, reaching 2% of the theoretical value, and the fermentation intensity was 0.4% per day.
[0042] Detecti...
Embodiment 3
[0043] The fermentation culture of embodiment 3L-(+)-tartaric acid
[0044] Fermentation medium: 5-keto-gluconate potassium 60g / L, MgSO 4 0.25g / L, KH 2 PO 4 0.6g / L, urea 2g / L, corn steep liquor 10g / L, its fermentation and detection condition are all with embodiment 2, fermentation time is 4d, and L-(+)-tartaric acid output is 1.2g / L, reaches the theoretical value 10%, with a fermentation intensity of 2.5% per day.
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