Enzyme-responsive self-aggregation luminous molecule and applications thereof in monitoring enzyme activity

A self-aggregation luminescent, enzyme-responsive technology, applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, to achieve excellent biocompatibility, strong biocompatibility, and avoid aggregation-induced luminescence quenching and the effect of photoquenching
CN103529017AActive Publication Date: 2014-01-22THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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Patent Information

Authority / Receiving Office
CN Ā· China
Patent Type
Applications(China)
Current Assignee / Owner
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Publication Date
2014-01-22

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Abstract

The invention discloses an enzyme-responsive self-aggregation luminous molecule and applications thereof in monitoring enzyme activity. The enzyme-responsive self-aggregation luminous molecule is formed by connecting a hydrolase substrate to an aggregation-induced luminous molecule, and can produce aggregation and induced luminescence after hydrolyzation under the action of the hydrolase. When the enzyme-responsive self-aggregation luminous molecule is added into living cells or extracellular reaction systems containing the corresponding hydrolase, the activity level of the hydrolase can be reflected through monitoring fluorescence signals by a fluorescence microscopic technology. The enzyme-responsive self-aggregation luminous molecule can be used for long-time space-time monitoring of the activity level of the hydrolase in cells.
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Description

technical field

[0001] The invention relates to the technical field of enzyme activity monitoring, in particular to an enzyme-responsive self-aggregation luminescent molecule and its application in monitoring enzyme activity. Background technique

[0002] Any cell is composed of dynamic liposomes, proteins, nucleic acids, sugars and other macromolecules and small molecules with precisely adjustable structures and functions and ordered structures. Their complexity and variety are far beyond our current understanding. But as we all know, the state of cells directly reflects the state of organisms, so human beings must try various ways to know and understand the ubiquitous organisms, including ourselves. Understanding the interaction between molecules or molecular assemblies in cells is a way for us to recognize cells, which not only helps us understand life phenomena, but also helps us better diagnose and correct ourselves. With the rapid development of computer simulation t...

Claims

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