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Alginic acid-hyaluronic acid in situ tissue engineering cell scaffold and its preparation method

A technology of hyaluronic acid and tissue engineering, applied in medical science, prosthesis, etc., can solve the problems of insufficient tissue support of natural materials, poor histocompatibility of polymer materials and inorganic materials, and rapid degradation, etc., to achieve Avoid toxicity risk, good safety and efficacy, effect of maintaining cell viability

Inactive Publication Date: 2014-02-05
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been many research reports on materials used in in situ tissue engineering scaffolds in vivo, such as hyaluronic acid, collagen, fibrin glue, chitosan, alginic acid, PLGA, hydroxyapatite, calcium sulfate, etc. Significantly improve the situ and survival rate of transplanted cells, but there are still deficiencies that need to be improved, such as insufficient tissue support and rapid degradation of natural materials, poor histocompatibility of polymer materials and inorganic materials, etc.

Method used

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  • Alginic acid-hyaluronic acid in situ tissue engineering cell scaffold and its preparation method
  • Alginic acid-hyaluronic acid in situ tissue engineering cell scaffold and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1. Preparation of a kind of alginic acid-hyaluronic acid in situ tissue engineering cell scaffold

[0064] Using sodium alginate and oxidized formylated hyaluronic acid as raw materials, the preparation steps are as follows:

[0065] 1) Preparation of solution

[0066] Preparation of solution 1: Weigh an appropriate amount of sodium alginate powder and prepare a 4% (w / v) solution with physiological saline; the weight-average molecular weight of the sodium alginate is about 150,000 Da, and the M / G ratio is about 0.65 .

[0067] Preparation of solution 2: Weigh an appropriate amount of oxidized formylated hyaluronic acid powder, and prepare a 3.5% (w / v) solution with physiological saline; the weight average molecular weight of the oxidized formylated hyaluronic acid is about 2 million Da , the degree of formylation is about 0.5.

[0068] 2) Preparation before use

[0069] A total of 10 cells to be loaded 6 Mix one with 0.5ml of the above solution 1, and fill ...

Embodiment 2

[0071] Example 2. Preparation of an alginic acid-hyaluronic acid in situ tissue engineering cell scaffold

[0072] Using oxidative alginate sodium alginate and sodium hyaluronate as raw materials, the preparation steps are as follows:

[0073] 1) Preparation of solution

[0074] Preparation of solution 1: Weigh an appropriate amount of oxidative alginate sodium alginate powder, and prepare a 4% (w / v) solution with physiological saline; the weight average molecular weight of the sodium alginate is about 150,000 Da, M / G The ratio is about 0.65 and the degree of aldylation is about 0.5.

[0075] Preparation of solution 2: Weigh an appropriate amount of sodium hyaluronate powder and prepare a 3.5% (w / v) solution with physiological saline; the weight average molecular weight of the sodium hyaluronate is about 2 million Da.

[0076] 2) Preparation before use

[0077] Mix the cells to be loaded with the above solution 1, and fill it into a sterile syringe; fill the above solution ...

Embodiment 3

[0079] Example 3. Preparation of an alginic acid-hyaluronic acid in situ tissue engineering cell scaffold

[0080] Using oxidative alginate sodium alginate and sodium hyaluronate as raw materials (the weight average molecular weight of the raw materials is the same as in Example 2), the preparation steps are the same as in Example 2, except that:

[0081] The concentration of solution 1 is 3.0% (w / v); the concentration of solution 2 is 2.5% (w / v);

[0082] Mix solution 1 and solution 2 with a volume ratio of 1:2 through a three-way syringe, and equilibrate at room temperature for 4 minutes to form the desired mixture of alginic acid-hyaluronic acid in situ tissue engineering cell scaffolds and cells to be loaded.

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Abstract

The invention provides an alginic acid-hyaluronic acid in situ tissue engineering cell scaffold and its preparation method. The preparation method comprises the following steps: alginic acid or its derivative and hyaluronic acid or its derivative are respectively made into solutions by the use of normal saline or physiological buffer; any one of the above solutions is mixed with cells which need to be loaded and then is mixed with the other remaining solution; and balancing lasts for a period of time at room temperature so as to obtain the alginic acid-hyaluronic acid in situ tissue engineering cell scaffold. The prepared alginic acid-hyaluronic acid in situ tissue engineering cell scaffold effectively prolongs degradation time of alginic acid and hyaluronic acid in vivo. By appropriate intramolecular crosslinking, mechanical properties and tissue filling / supporting performance of the scaffold are raised greatly. The scaffold has good security and validity. The preparation method of the alginic acid-hyaluronic acid in situ tissue engineering cell scaffold does not involve any organic reagent; the reaction system is simple and mild; and clinic application security is good.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to an in-situ tissue engineering cell scaffold and a preparation method thereof. Background technique [0002] With the development of tissue engineering and regenerative medicine, cell transplantation or cell therapy has gradually become a research hotspot in clinical treatment. Implanting autologous or allogeneic cells into diseased tissues can replace or partially restore body functions, but without any side effects on normal cells or tissues, which is the advantage of cell transplantation over conventional treatments. Many studies have extensively discussed cell transplantation, and pancreatic islet cell transplantation has achieved good clinical results in the treatment of diabetes. It has also been reported that stem cell transplantation has made breakthroughs in clinical problems that are difficult to solve in traditional medicine such as tumors and neurologica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/42A61L27/38A61L27/26A61L27/54
Inventor 位晓娟奚廷斐顾其胜郑玉峰
Owner PEKING UNIV
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