Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for producing sophorolipids by fermenting waste molasses and gutter oil as two carbon sources

A technology of gutter oil and sophorolipids, which is applied in the field of biosurfactant preparation, to achieve the effects of ensuring health, facilitating development, and having high application value

Active Publication Date: 2016-01-13
QINGDAO UNIV OF SCI & TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, reports on the use of waste oil as a hydrophobic carbon source focus on the detection method on the one hand, and on the esterification and saponification of biodiesel and fatty acids on the other hand. There is no report on fermentation as a substrate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Torulopsis globosa ( Torulopsis bombicola ) ATCC22214 was inoculated on the YPD slant medium, cultured at 30°C for 2 days, and the bacteria were eluted with 0.85% normal saline, and the cell content was about 5.3×10 7 Individual / mL bacterial suspension;

[0029] (2) Preparation of seed liquid: composition of seed medium (g / L): glucose 60, yeast powder 5, urea 0.5, pH 6.0; 100 mL of the seed medium was placed in a 500 mL shaker flask, sterilized at 121° C. for 20 min, After cooling, add the above-mentioned bacterial suspension according to the inoculum amount of 3% by volume, place the shaker flask at 25°C, and cultivate it on a shaker with a rotation speed of 120rpm for 24h to obtain a seed solution;

[0030] (3) The above-mentioned seed solution is used to inoculate the fermentation medium in a sterile 5L fermenter with waste molasses and waste oil as substrates. Fermentation medium composition (g / L): waste oil 60, waste molasses 60, yeast powder 3, pH=6.0, tap ...

Embodiment 2

[0033] (1) Torulopsis globosa ( Torulopsis bombicola ) ATCC22214 was inoculated on the YPD slant medium, cultured at 30°C for 2 days, and the bacteria were eluted with 0.85% normal saline, and the cell content was about 6.2×10 7 Individual / mL bacterial suspension;

[0034] (2) Preparation of seed liquid: composition of seed medium (g / L): glucose 80, yeast powder 6, urea 0.8, pH 6.0; 100mL of the seed medium was placed in a 500mL shaker flask, sterilized at 121°C for 20min, After cooling, insert the above-mentioned bacterial suspension according to the inoculum amount of 4% by volume, place the shaker flask at 28°C, and cultivate it on a shaking table with a rotation speed of 150rpm for 24 hours to obtain a seed solution;

[0035] (3) The above seed liquid is used to inoculate a sterile 5L fermenter with waste molasses and waste oil as substrates in the fermentation medium. Fermentation medium composition (g / L): waste oil 80, waste molasses 100, yeast powder 4, pH=6.0, tap w...

Embodiment 3

[0038] (1) Torulopsis globosa ( Torulopsis bombicola ) ATCC22214 was inoculated on the YPD slant medium, cultured at 30°C for 2 days, and the bacteria were eluted with 0.85% normal saline, and the cell content was about 7.4×10 7 Individual / mL bacterial suspension;

[0039] (2) Preparation of seed liquid: composition of seed medium (g / L): glucose 100, yeast powder 7, urea 1, pH 6.0; 100mL of the seed medium was placed in a 500mL shake flask, sterilized at 121°C for 20min, After cooling, add the above-mentioned bacterial suspension according to the inoculation amount of 5% by volume, and place the shaker flask at 30°C with a rotating speed of 180rpm for 24 hours to obtain the seed solution;

[0040](3) The above seed liquid is used to inoculate a sterile 5L fermenter with waste molasses and waste oil as substrates in the fermentation medium. Fermentation medium composition (g / L): waste oil 100, waste molasses 160, yeast powder 4, pH=6.0, tap water to a volume of 3L, sterilize...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for fermenting and producing sophorolipids from waste molasses and waste oil as dual carbon sources. Bacterial suspension (2) Preparation of seed solution (3) Inoculation of seed solution in fermentation medium with waste molasses and gutter oil as substrates for fermentation (4) Extraction of sophorolipids with ethyl acetate. The invention realizes resource utilization of wastes and greatly reduces the production cost of sophorolipids. It solves the problem of heavy waste oil discharge pollution and great harm when re-entering the edible field, and realizes the rational utilization of waste oil resources. The process does not require complex equipment, and the fermentation process is simple and easy, which is beneficial to the development, application and popularization of the sophorolipid.

Description

technical field [0001] The invention belongs to the technical field of preparation of biosurfactants, and in particular relates to a method for fermenting and producing sophorolipids from waste molasses and waste oil as dual carbon sources. Background technique [0002] In addition to being used as detergents in daily life, surfactants can be used in almost all fine chemical fields. Sophorolipid is an environmentally friendly biosurfactant produced by microorganisms. Compared with surfactants produced by chemical synthesis or petroleum refining, it has its own advantages in addition to its common characteristics. Sophorolipids are glycolipid-based biosurfactants with low toxicity, good compatibility with the human body and the environment, and good emulsification, dispersion, and solubilization properties; they are used in food, medicine, cosmetics, metallurgy, environmental protection, waste Oil recovery and other fields have research and development value. Sophorolipids ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/12C12R1/645
Inventor 李俊峰梁生康李红芳段效辉
Owner QINGDAO UNIV OF SCI & TECH