Automatic control method and system for pH/oxygen partial pressure/partial pressure of carbon dioxide of cell and tissue culture solution

Abstract

The invention provides an automatic control method and system for pH / oxygen partial pressure / partial pressure of carbon dioxide of a cell and tissue culture solution. The system comprises a culture room, a central control unit, a material exchanger, a nitrogen source, an oxygen source and a carbon dioxide source, wherein a circulating culture solution pipeline is arranged between the culture room and the material exchanger; a biochemical sensor is arranged on an upstream circulating culture solution pipeline between the upstream end of the culture room and the circulating culture solution outlet of the material exchanger, and a first liquid driving device is arranged on a downstream circulating culture solution pipeline between the downstream end of the culture room and the circulating culture solution inlet of the material exchanger; the nitrogen source, the oxygen source and the carbon dioxide source are respectively connected with the gas inlet of the material exchanger through a solenoid valve and gas pipelines; the biochemical sensor, the first liquid driving device and the solenoid valve are electrically connected with the central control unit respectively. The method and the system realize long-term culture of tissues under the totally-enclosed condition.

Description

technical field [0001] The invention relates to the field of biomedical engineering cell and tissue cultivation, in particular to an automatic control method and system for pH / oxygen partial pressure / carbon dioxide partial pressure of cell and tissue culture fluid. Background technique [0002] In the in vitro culture environment, cells and tissues continuously consume O during the growth process. 2 , producing CO 2 And accumulate in the culture solution, so that the pH of the culture solution continues to drop. [0003] The conventional method of maintaining a stable pH is to discard the old medium and add new medium, known as a medium change. The method of changing the liquid is carried out when the pH value drops to a relatively low level, resulting in large fluctuations in the biochemical environment, and frequent liquid changes will greatly increase the risk of contamination. [0004] Sufficient gas exchange is required to maintain the oxygen partial pressure / carbon ...

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Problems solved by technology

In addition to increasing the risk of contamination, this method also interrupts the continuous growth process of the tissue

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