Method for extracting and separating high-purity phlorizin from Cynomorium songaricum

A phloridzin and high-purity technology, which is applied in the field of extracting and separating high-purity phloridzin from Cynomorium syringae, can solve problems such as difficulty in separation, and achieve the effects of simple separation operation process, easy control and high purification efficiency.

Inactive Publication Date: 2014-03-12
ENSHI JINHUA BIOLOGICAL ENG
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of phlorizin from Rosaceae plants such as apples is now facing the problem of separation difficulties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0022] A method for extracting and separating high-purity phlorizin from Cynomorium cynomorium, comprising the following steps:

[0023] (1) Crush and dry 50 parts Cynomorium, set aside;

[0024] (2) Add water, extract twice, add 400 parts of solvent for the first time, heat to boiling, extraction time is 3 hours; add 300 parts of solvent for the second time, heat to boiling, extraction time is 2 hours; combine two extractions , filtered to obtain the filtrate;

[0025] (3) Put 100 parts of the obtained filtrate on the D101 macroporous adsorption resin to exchange resin, put it on the column for 30 minutes, and then use 100 parts of the upper column liquid to elute with 20% ethanol, collect the effluent and put it on the column again, and then use 200 parts of the upper column The column solution was eluted with 70% ethanol, and the eluate was collected and concentrated to obtain 15 parts;

[0026] (4) Repeatedly extract the concentrated eluate with petroleum ether until the...

Embodiment example 2

[0035] A method for extracting and separating high-purity phlorizin from Cynomorium cynomorium, comprising the following steps:

[0036] (1) Crush and dry 80 parts Cynomorium, set aside;

[0037] (2) Add water, extract twice, add 640 parts of solvent for the first time, heat to boiling, extraction time is 3 hours; add 420 parts of solvent for the second time, heat to boiling, extraction time is 2 hours; combine two extractions , filtered to obtain the filtrate;

[0038] (3) Put 150 parts of the obtained filtrate on the D101 macroporous adsorption resin to exchange the resin, put it on the column for 30 minutes, and then use 160 parts of the upper column solution to elute with 20% ethanol, collect the effluent and put it on the column again, and then use 320 parts of the upper column solution The column solution was eluted with 70% ethanol, and the eluate was collected and concentrated to obtain 25 parts;

[0039] (4) Repeatedly extract the concentrated eluate with petroleum ...

Embodiment example 3

[0048] A method for extracting and separating high-purity phlorizin from Cynomorium cynomorium, comprising the following steps:

[0049](1) Crush and dry Cynomorium 100 parts, set aside;

[0050] (2) Add water, extract twice, add 800 parts of solvent for the first time, heat to boiling, extraction time is 3 hours; add 600 parts of solvent for the second time, heat to boiling, extraction time is 2 hours; combine two extractions , filtered to obtain the filtrate;

[0051] (3) Put 200 parts of the obtained filtrate on the D101 macroporous adsorption resin to exchange the resin, put it on the column for 30 minutes, and then use 200 parts of the upper column liquid to elute with 20% ethanol, collect the effluent and put it on the column again, and then use 400 parts of the upper column The column liquid was eluted with 70% ethanol, and the eluate was collected and concentrated to obtain 35 parts;

[0052] (4) Repeatedly extract the concentrated eluate with petroleum ether until t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting and separating high-purity phlorizin from Cynomorium songaricum. The method comprises the following steps: (1) smashing dry Cynomorium songaricum for later use; (2) adding water, and filtering to obtain filtrate after extraction; (3) treating the obtained filtrate with a D101 macroporous adsorption resin exchange resin, eluting with an eluting liquid, collecting and concentrating the eluant; (4) repeatedly extracting the concentrated eluant, and then, concentrating supernatant extract liquor, thus obtaining a crude phlorizin product; (5) dissolving the crude phlorizin product with ethyl acetate, adding weakly acidic activated carbon, filtering while hot, cooling and crystallizing to obtain crude phlorizin crystals; (6) adding ethyl acetate into the crude phlorizin crystals, concentrating and cooling the crystals to obtain the phlorizin product. According to the method, requirements on environment condition are not high in the whole separation process, separation time is relatively short, and purity is very high. Separation materials are easy to obtain and cheap, and operation process of separation is simple and easy to control; the ethyl acetate is used for crystallization and recrystallization, so that purification efficiency is high; the reagents used are non-toxic.

Description

[0001] technical field [0002] The invention is specifically a method for extracting and separating high-purity phlorizin from Cynomorium cynomorium. Background technique [0003] Phlorizin belongs to plant polyphenolic compounds. There are many studies abroad on the physiological functions of phloridizin. Phlorizin can reduce fire, among which cellular protein kinase can inhibit disordered proliferation of cells, and is an anti-mutagenic factor, which can be used as adjuvant therapy for skin liver cancer and other tumors. Correlation studies on the content of phloridizin show that phloridizin can effectively slow down the proliferation and growth of colon cancer cell nuclei and liver tumor cells; it has strong antioxidant properties, and the antioxidant concentration of oil is between 10-30ppm; It has a significant antihypertensive and lipid-lowering effect, and is known as a "natural antihypertensive drug". It is especially suitable for patients with hypertension or hyp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
Inventor 彭炜石永进吴红丽
Owner ENSHI JINHUA BIOLOGICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap