Screening of aptamers specially targeting gastric cancer cells and aptamers obtained after screening

Abstract

The invention discloses screening of aptamers specially targeting gastric cancer cells and aptamers obtained after screening, and belongs to the fields of bioanalysis and genetic engineering. The screening method provided by the invention is based on a cell-SELEX technology, SGC 7901 cells human gastric carcinoma cells are adopted as target cells, GES-1 cells of human gastric mucosa cells are adopted as negative-screening cells; during the negative-screening, the amount, elution time and volume of eluant are continuously increased; meanwhile, the incubating time of target cells is gradually shortened, and the incubating time of negative-screening cells is properly prolonged; through screening for several times, a sequence specially bound with the gastric cancer cells and high in binding force can be obtained, namely, the aptamers for special targeted gastric cancer cells can be obtained. The sequences for the aptamers are shown in SEQ ID NO.1-NO.6, and the aptaamers have good binding force and specificity, can be bound with and target the gastric cancer cells specially, and can be used for diagnosis of gastric cancer and preparation of gastric cancer diagnosis drugs.

Description

[0001] technical field [0002] The invention belongs to the fields of biological analysis and genetic engineering, and in particular relates to the screening of aptamers specifically targeting gastric cancer cells and the aptamers obtained from the screening. Background technique [0003] Nucleic acid aptamers are screened from random single-stranded oligonucleotide libraries using systematic evolution of ligands by exponential enrichment (SELEX) technology, which can bind to target substances with high specificity and high affinity. ligand. Cell SELEX (cell-SELEX) is a nucleotide sequence that can bind to target cells with high specificity and high affinity, and is screened from a synthetic oligonucleotide library based on intact cells. [0004] cell-SELEX is superior to traditional SELEX technology in target screening, it can better maintain the integrity and biological activity of target molecules on the cell surface, and is more conducive to the analysis and detecti...

AI Technical Summary

Problems solved by technology

[0005] Gastric cancer is one of the malignant tumors with a relatively high incidence in the world, and most of the gastric cancers that can be diagnosed clinically are in the middle and advanced stages, lacking suitable and effective treatment methods, so some tumor markers on gastric cancer cells have been found (It may also be a treatment gene) is of great significance to the early diagnosis of gastric cancer and the design of reasonable and effective treatment methods

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