Preparation method of red algae sterile explant

Abstract

The invention discloses a preparation method of red algae sterile explant. The preparation method is characterized by comprising the steps of cleaning red algae by utilizing sterile filtered seawater, immersing one of gracilaria verrucosa, grateloupia filicina, gelidium amansii, gelidiella acerosa, asparagus, carrageen and pelvetia silquosa in 1% to 5% potassium iodide solution for 1min to 4min, and then washing by utilizing the sterile filtered seawater; immersing the red algae in 1% to 5% povidone iodine for 2min to 8min, and then cleaning the red algae by utilizing sterile filtered seawater; finally immersing the algae in 1% to 3% sodium hypochlorite solution for 1min to 5min, and cleaning the red algae by utilizing the sterile filtered seawater to obtain the sterile red algae explant. The potassium iodide, povidone iodine and sodium hypochlorite are adopted as sterilizing agents, so that the bacteria and microorganisms on the surface of the red algae explant can be effectively killed, the growth of the plant is not injured, and 80% sterile feasible explant can be obtained.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for preparing a sterile explant of red algae. Background technique [0002] Plant tissue culture technology refers to the culture of isolated plant organs, tissues, cells, embryos and protoplasts on artificially prepared medium under sterile culture conditions, and induces the production of callus or Latent buds, or the technique of growing into a complete plant; since the object of cultivation is the part that is separated from the mother and is cultivated in a test tube, it can also be called in vitro culture. Plant tissue culture technology is not limited by seasons and geographical conditions. The propagation speed is fast, the source of plant materials is single, the genetic background is consistent, and it has good repeatability. The in vitro rapid propagation technology can rapidly expand the plant's growth rate in a short period of time. quantity. ...

AI Technical Summary

Problems solved by technology

Compared with higher terrestrial plant tissue culture technology, the research on marine plant tissue culture technology is relatively backward. The biggest problem facing seaweed tissue culture is that it is impossible to obtain sterile and viable seaweed explants, because marine plants are composed of single or multi-layer It is composed of cells, and its organizational structure is very different from that of terrestrial plants. It does not have the vascular structure of stems and leaves of higher plants, nor does it have hard epidermis protection; disinfectants such as alcohol and mercury chloride commonly used in terrestrial plants are very Easily invades the interior of algae cells and causes explant death

At present, the tissue culture technology of macroalgae is relatively backward. According to the known data, antibiotic soaking or detergent cleaning are commonly used to achieve the purpose of disinfection and sterilization. However, detergent disinfection and cleaning are very difficult problems, and antibiotics have many hazards. It is not known what kind of harm to the treated explants and regenerated plants. The feasibility of the above-mentioned treatment of red algae explants is very low, and only 60% of sterile explants can be obtained at most.