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Preparation method of compound microbial fertilizer

A technology of compound microorganisms and fertilizers, applied in the biological field, can solve problems such as pollution, compaction, and soil acidification

Inactive Publication Date: 2014-03-26
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, a large amount of chemical fertilizers are used in agricultural production, so that nutrients such as nitrogen and phosphorus enter the water body in large quantities, causing eutrophication of the water body and causing non-point source pollution of surface water by chemical fertilizers.
Third, long-term use of chemical fertilizers will cause soil acidification and compaction, resulting in a decline in soil fertility

Method used

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  • Preparation method of compound microbial fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Add the ground peat soil powder into calcium carbonate at 9:1 (M:M) and then carry out high pressure (1.034×10 5 Pa) Sterilize twice (121°C, 30min), dry for later use;

[0030] (2) The ground vermicompost is subjected to high pressure (1.034×10 5 Pa) Sterilize twice (121°C, 30min), dry for later use;

[0031] (3) Preparation of liquid medium: peptone 20g, glycerol 10mL, K 2 HPO 4 1.5g, MgSO 4 ·7H 2 O1.5g, deionized water 1L, after mixing, sterilize at 121°C for 20min;

[0032] (4) Preparing protective agent solution: 0.5wt% sodium alginate, 15wt% glycerin and 0.1wt% sodium carboxymethyl cellulose; 1L deionized water, sterilized at 121°C for 20min and stored at room temperature;

[0033] (5) Pick a single colony of Pseudomonas citricum JD37 into 50mL liquid KB medium, culture at 28°C and 200rpm for 18 hours, then take 10mL of fermentation broth in 1000mL fresh liquid KB medium according to the inoculum size of 1%, and shake culture To the logarithmic phase (28...

Embodiment 2

[0036] (1) Add the ground peat soil powder into calcium carbonate at 9:1 (M:M) and then carry out high pressure (1.034×10 5 Pa) Sterilize twice (121°C, 30min), dry for later use;

[0037] (2) The ground vermicompost is subjected to high pressure (1.034×10 5 Pa) Sterilize twice (121°C, 30min), dry for later use;

[0038] (3) Preparation of liquid medium: peptone 20g, glycerin 10mL, K 2 HPO 4 1.5g, MgSO 4 ·7H 2 O1.5g, deionized water 1L, after mixing, sterilize at 121°C for 20min;

[0039] (4) Preparing protective agent solution: 0.5wt% sodium alginate, 15wt% glycerin and 0.1wt% sodium carboxymethyl cellulose; 1L deionized water, sterilized at 121°C for 20min and stored at room temperature;

[0040](5) Pick a single colony of Pseudomonas fluorescens P13 into 50 mL of liquid KB medium, culture it at 28°C and 200 rpm for 20 hours, then take 10 mL of fermentation liquid in 1000 mL of fresh liquid KB medium according to the inoculum size of 1%, and shake it until In the logar...

Embodiment 3

[0043] (1) Add the ground peat soil powder into calcium carbonate at 9:1 (M:M) and then carry out high pressure (1.034×10 5 Pa) Sterilize twice (121°C, 30min), dry for later use;

[0044] (2) The ground vermicompost is subjected to high pressure (1.034×10 5 Pa) Sterilize twice (121°C, 30min), dry for later use;

[0045] (3) Preparation of liquid medium: peptone 20g, glycerol 10mL, K 2 HPO 4 1.5g, MgSO 4 ·7H 2 O1.5g, deionized water 1L, after mixing, sterilize at 121°C for 20min;

[0046] (4) Preparing protective agent solution: 0.5wt% sodium alginate, 15wt% glycerin and 0.1wt% sodium carboxymethyl cellulose; 1L deionized water, sterilized at 121°C for 20min and stored at room temperature;

[0047] (5) Pick a single colony of Pseudomonas citrus JD37 into 50 mL of liquid KB medium, culture at 28°C and 200rpm for 18 hours, pick a single colony of Pseudomonas fluorescens P13 into 50mL of liquid KB medium, and culture at 28°C and 200rpm After culturing at 200rpm for 20h, tak...

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Abstract

The invention discloses a preparation method of a compound microbial fertilizer. The method comprises the following steps: inoculating pseudomonas aurantiaca JD37 or pseudomonas fluorescens P13 into a liquid culture medium; carrying out shake cultivation at 28 DEG C for 16-20 hours at the speed of 200r / min; taking thalli after centrifuging, suspending and evenly mixing with a sterilized protective agent solution, so as to obtain a suspended bacterium fluid; mixing the suspended bacterium fluid with a sterilized matrix, wherein the mass-to-volume ratio of the matrix to the suspended bacterium fluid is 1-2g / ml; and mixing the matrix mixed with the suspended bacterium fluid with the sterilized commercially available wormcast according to the mass ratio of 1:1. By adopting the preparation method, two plant growth-promoting bacteria are combined, so that growth of the crop can be facilitated, a few of phytopathogens also can be prevented and treated, a composite protective agent applicable to the preparation method is provided, the effect is more significant than that of a single protective agent, the matrix which is low in cost and easy to obtain is selected, no pollution or harm is generated to the environment, and the soil quality can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of compound microbial fertilizer. Background technique [0002] In order to pursue high yields, traditional agriculture uses chemical fertilizers as a source of fertility for crops, and the problems in the long-term use of chemical fertilizers have become more and more prominent. There are three main aspects: First, the mineral raw materials and chemical raw materials used to make fertilizers contain a variety of heavy metal radioactive substances and other harmful components. They enter the farmland soil with fertilization and cause heavy metal pollution. Second, a large amount of chemical fertilizers are used in agricultural production, so that nutrients such as nitrogen and phosphorus enter the water body in large quantities, causing eutrophication of the water body and causing non-point source pollution of surface water by chemical fertilizers. It is estimated...

Claims

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Application Information

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IPC IPC(8): C05F15/00
Inventor 肖明周晨昊蒋秋悦陈兵
Owner SHANGHAI NORMAL UNIVERSITY
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